EC 3.1.1.32
EC 3.1.1.4
Detergent-resistant phospholipase A
DR-phospholipase A
Phosphatidylcholine 1-acylhydrolase
Outer membrane phospholipase A
OM PLA

Gene name

Name:

pldA

From

Proteus vulgaris

[TaxID: 585]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Proteus.

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8300539 [NCBI, ExPASy, EBI, Israel, Japan]
Brok R.G.P.M., Brinkman E., van Boxtel R., Bekkers A.C.A.P., Verheij H.M., Tommassen J.;
"Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A.";
J. Bacteriol. 176:861-870(1994).

Comments

FUNCTION: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
CATALYTIC ACTIVITY: Phosphatidylcholine + H₂O = 2-acylglycerophosphocholine + a carboxylate.
CATALYTIC ACTIVITY: Phosphatidylcholine + H₂O = 1-acylglycerophosphocholine + a carboxylate.
COFACTOR: Calcium.
SUBCELLULAR LOCATION: Cell outer membrane. Note=One of the very few enzymes located there.
SIMILARITY: Belongs to the phospholipase A1 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X76902; CAA54224.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

C36971; C36971.

3D structure databases

HSSP

P00631; 1QD6. [HSSP ENTRY / PDB]

SMR

P37447; 28-289.

ModBase

P37447.

Ontologies

GO:0008970;	Molecular function: phospholipase A1 activity (inferred from electronic annotation from EC).
GO:0004623;	Molecular function: phospholipase A2 activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR003187; PLA1.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.230.10; PLA1; 1.

Pfam

PF02253; PLA1; 1.
Pfam graphical view of domain structure.

PRINTS

PR01486; PHPHLIPASEA1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Cell membrane; Cell outer membrane; Hydrolase; Lipid degradation; Membrane; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 20`	`20`	`By similarity.`
`CHAIN`	`21 289`	`269`	`Phospholipase A1.`	`PRO_0000021987`
`ACT_SITE`	`164 164`		`By similarity.`

Sequence information

Length: 289 AA [This is the length of the unprocessed precursor]

Molecular weight: 32945 Da [This is the MW of the unprocessed precursor]

CRC64: D75516CFFB406997 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRTGPGWLLA AAALPFFACA QEATIDKVHD TPAVRGSIIA NMLQEHDNPF TLYPYESNYL 

        70         80         90        100        110        120 
LYTYTSDLNK KAIESYNWSD NANKDEVKFQ LSLAFPLWRG ILGDNSLLGA SYTQRSWWQL 

       130        140        150        160        170        180 
SNTGESAPFR ETNYEPQLFL GFATDYSVGD WTLRDAEFGY NHQSNGRSDP TSRSWNRLYS 

       190        200        210        220        230        240 
RLMAQNGNWL VEVKPWYVIG DTSDNKNITK YMGYYQLKIG YQLGEAVLSA KGQYNWNTGY 

       250        260        270        280 
GGAELGVSYP ITKHVRFYTQ VYSGYGESLI DYDFNQTRVG MGVMLNDLF

P37447 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools