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UniProtKB/Swiss-Prot entry P37225

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAON_SOLTU
Primary accession number P37225
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1994
Sequence was last modified on October 1, 1994 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 57)
Name and origin of the protein
Protein name NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Precursor]
Synonyms NAD-ME
EC 1.1.1.39
Gene name None
From
Solanum tuberosum (Potato) [TaxID: 4113] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. Desiree;
TISSUE=Leaf;
PubMed=8106447 [NCBI, ExPASy, EBI, Israel, Japan]
Winning B.M., Bourguignon J., Leaver C.J.;
"Plant mitochondrial NAD+-dependent malic enzyme. cDNA cloning, deduced primary structure of the 59- and 62-kDa subunits, import, gene complexity and expression analysis.";
J. Biol. Chem. 269:4780-4786(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z23002; CAA80547.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A53318; A53318.
3D structure databases
HSSP P27443; 1LLQ. [HSSP ENTRY / PDB]
ModBase P37225.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P37225.
Other
ProtoNet P37225.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    18  18     Mitochondrion. 
CHAIN   19   601  583     NAD-dependent malic enzyme 59 kDa isoform, mitochondrial. PRO_0000018544
ACT_SITE   129   129        Proton donor (By similarity). 
ACT_SITE   200   200        Proton acceptor (By similarity). 
METAL   271   271        Divalent metal cation (By similarity). 
METAL   272   272        Divalent metal cation (By similarity). 
METAL   295   295        Divalent metal cation (By similarity). 
BINDING   182   182        NAD (By similarity). 
BINDING   295   295        NAD (By similarity). 
BINDING   444   444        NAD (By similarity). 
SITE   295   295  1     Important for activity (By similarity). 
Sequence information
Length: 601 AA [This is the length of the unprocessed precursor] Molecular weight: 66272 Da [This is the MW of the unprocessed precursor] CRC64: D6C2A128CC9E971C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MWRVARSAAS TFRRTRRLST AISAPCIVHK RGADILHDPW FNKDTGFPMT ERDRLGLRGL 

        70         80         90        100        110        120 
LPPRVISFEQ QYDRFMESFR SLEKNTEGQP DSVVSLAKWR ILNRLHDRNE TLYYRVLIDN 

       130        140        150        160        170        180 
IKDFAPIIYT PTVGLVCQNY SGLFRRPRGM YFSAKDKGEM MSMIFNWPST QVDMIVLTDG 

       190        200        210        220        230        240 
SRILGLGDLG VQGIGIPIGK LDMYVAAAGI NPQRVLPVML DVGTNNQKLL EDPLYLGLRQ 

       250        260        270        280        290        300 
PRLEGEEYLS IVDEFVEAVH ARWPKAVVQF EDFQAKWAFE TLDRYRKKFC MFNDDIQGTA 

       310        320        330        340        350        360 
GVALAGLLGT VRAQGRPLTD FANQKIVVVG AGSAGLGVLK MALQAVSRMT GPSADPHFFL 

       370        380        390        400        410        420 
LDKNGLITKD RKDIDPAALP FAKAHHEIEG LGLQEGAGLA EVVKKVKPHV LLGLSGVGGI 

       430        440        450        460        470        480 
FHEEVLRAMK ESDSVRPAIF AMSNPTNNAE CCPVDAFKLA GEDIVFASGS PFANVDLGNG 

       490        500        510        520        530        540 
KIGHVNQANN MYLFPGIGLG ALLSGARNIS DTMLEAAAEC LASYMSDDEI NRGILYPSID 

       550        560        570        580        590        600 
DIRDITAEVG AAVLRAAVAE DLAEGHGDVG VKELQHMSKE ETIEHVRQNM WYPVYGPLVH 


E
P37225 in FASTA format

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