[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Rutgers; Finger F.L., Knee M., Lagrimini L.M.A.; "Isolation of a cDNA clone for tomato fruit NADP+ malic enzyme."; Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO(2) released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO(2) that occurs in most plants.
CATALYTIC ACTIVITY: (S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH.
COFACTOR: Divalent metal cations. Prefers magnesium or manganese (By similarity).
PATHWAY: Photosynthesis; C4 acid pathway .
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast.
SIMILARITY: Belongs to the malic enzymes family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L27509; AAA34174.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T07088; T07088.

3D structure databases

HSSP

P40927; 1GQ2. [HSSP ENTRY / PDB]

ModBase

P37222.

Family and domain databases

InterPro

IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.

PRINTS

PR00072; MALOXRDTASE.

PROSITE

PS00331; MALIC_ENZYMES; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chloroplast; Metal-binding; NAD; NADP; Oxidoreductase; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 573`	`>573`	`NADP-dependent malic enzyme, chloroplastic.`	`PRO_0000160199`
`NP_BIND`	`318 334`	`17`	`NADP (By similarity).`
`ACT_SITE`	`123 123`		`Proton donor (By similarity).`
`ACT_SITE`	`194 194`		`Proton acceptor (By similarity).`
`METAL`	`265 265`		`Divalent metal cation (By similarity).`
`METAL`	`266 266`		`Divalent metal cation (By similarity).`
`METAL`	`289 289`		`Divalent metal cation (By similarity).`
`BINDING`	`176 176`		`NAD (By similarity).`
`BINDING`	`289 289`		`NAD (By similarity).`
`BINDING`	`430 430`		`NAD (By similarity).`
`SITE`	`289 289`	`1`	`Important for activity (By similarity).`
`NON_TER`	`1 1`

Sequence information

Length: 573 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 63561 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 13846E61206F8E75 [This is a checksum on the sequence]

        10         20         30         40         50         60 
IRHESTVTGG VQDVYGEDSA TEDQSITPWT LSVASGFSLL RNPHYNKGLA FSERERDTHY 

        70         80         90        100        110        120 
LRGLLPPVVI SHDLQVKKMM NSIRKYDVPL QRYMAMMDLQ EMNERLFYKL LIDNVEELLP 

       130        140        150        160        170        180 
IVYTPTVGEA CQKYGWIFKR PQGLFFSLKE KGKIHEVLKN WPEKKIQVIV VTDGERILGL 

       190        200        210        220        230        240 
GDLGCQGMGI PVGKLSLYSA LGGIRPSACL PVTIDVGQTM KFVDDEFYIG LRQRRATGQE 

       250        260        270        280        290        300 
YSELLDEFMY AVKQNYGEKV LIQFEDFANH NAFNLLAKYG TSHLVFNDDI QGTASVVLAG 

       310        320        330        340        350        360 
LMAALNLVGG SLSEHTFLFL GAGEAGTGIA ELIALEMSKQ TGIPLEETRK KIWMVDSKGL 

       370        380        390        400        410        420 
IVKSRMEMLQ HFKRPWAHDH EPVQELVNAV KSIKPTVLIG SSGAGRTFTK EVVQAMATFN 

       430        440        450        460        470        480 
EKPIIFALSN PTSQSECTAE EAYSWSEGRA IFASGSPFAP VEYNGKVYAS GQANNAYIFP 

       490        500        510        520        530        540 
GFGLGLIISG AIRVHDDMLL VASEALADEV SQENFEKGTH IPPFSNIRKI SAHIAKVAAK 

       550        560        570 
AYELGLATRL PQPKDLVAYA ESCMYSPAYR SYR

P37222 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools