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UniProtKB/Swiss-Prot entry P37221

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOM_SOLTU
Primary accession number P37221
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1994
Sequence was last modified on October 1, 1994 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 56)
Name and origin of the protein
Protein name NAD-dependent malic enzyme 62 kDa isoform, mitochondrial [Precursor]
Synonyms NAD-ME
EC 1.1.1.39
Gene name None
From
Solanum tuberosum (Potato) [TaxID: 4113] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. Desiree;
TISSUE=Leaf;
PubMed=8106447 [NCBI, ExPASy, EBI, Israel, Japan]
Winning B.M., Bourguignon J., Leaver C.J.;
"Plant mitochondrial NAD+-dependent malic enzyme. cDNA cloning, deduced primary structure of the 59- and 62-kDa subunits, import, gene complexity and expression analysis.";
J. Biol. Chem. 269:4780-4786(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z23023; CAA80559.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B53318; B53318.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase P37221.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P37221.
Other
ProtoNet P37221.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    34  34     Mitochondrion. 
CHAIN   35   626  592     NAD-dependent malic enzyme 62 kDa isoform, mitochondrial. PRO_0000018543
ACT_SITE   146   146        Proton donor (By similarity). 
ACT_SITE   217   217        Proton acceptor (By similarity). 
METAL   288   288        Divalent metal cation (By similarity). 
METAL   289   289        Divalent metal cation (By similarity). 
METAL   312   312        Divalent metal cation (By similarity). 
BINDING   199   199        NAD (By similarity). 
BINDING   312   312        NAD (By similarity). 
BINDING   467   467        NAD (By similarity). 
SITE   312   312  1     Important for activity (By similarity). 
Sequence information
Length: 626 AA [This is the length of the unprocessed precursor] Molecular weight: 69953 Da [This is the MW of the unprocessed precursor] CRC64: 8548B532B70A917C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAIFSNQMRL SSTLLKRLHQ RVAAAVNSSS SRNFTTTEGH RPTIVHKRSL DILHDPWFNK 

        70         80         90        100        110        120 
GTAFSFTERD RLHIRGLLPP NVMSFEQQIA RFMADLKRLE VQARDGPSDP YVLAKWRILN 

       130        140        150        160        170        180 
RLHDRNETLY YKVLMENIEE YAPIVYTPTV GLVCQKYSGL FRRPRGMYFS AEDRGEMMSM 

       190        200        210        220        230        240 
VYNWPADQVD MIVVTDGSRI LGLGDLGIQG IGIAIGKLDL YVAAAGINPQ RVLPVMIDVG 

       250        260        270        280        290        300 
TDNENLLKDP LYLGLQDHRL DGEEYIEVID EFMEAVFTRW PHVIVQFEDF QSKWAFKLLQ 

       310        320        330        340        350        360 
RYRNNYRMFN DDIQGTAGVA IAGLLGAVRA QGRPMIDFPK MKIVVAGAGS AGIGVLNAAR 

       370        380        390        400        410        420 
KTMARMLGNT EIAFESARSQ FWVVDAKGLI TEARENVDPD ARPFARKIKE IERQGLSEGA 

       430        440        450        460        470        480 
TLAEVVREVK PDVLLGLSAC GGLFSKEVLE ALKHSTSTRP AIFPMSNPTR NAECTPEEAF 

       490        500        510        520        530        540 
SILGENIIFA SGSPFKDVDL GNGHVGHCNQ ANNMFLFPGI GLGTLLSGSR IVSDGMLQAA 

       550        560        570        580        590        600 
AECLAAYITE EEVLKGIIYP SISRIRDITK EVAAAVVKEA IEEDLAEGYR EMDSRELRKL 

       610        620 
DEAQISEFVE NNMWSPDYPT LVYKKD
P37221 in FASTA format

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