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UniProtKB/Swiss-Prot entry P36013


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOM_YEAST
Primary accession number P36013
Secondary accession numbers None
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on June 1, 1994 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 66)
Name and origin of the protein
Protein name NAD-dependent malic enzyme, mitochondrial [Precursor]
Synonyms NAD-ME
EC 1.1.1.38
Gene name
Name: MAE1
OrderedLocusNames: YKL029C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
DOI=10.1038/369371a0; PubMed=8196765 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
"Complete DNA sequence of yeast chromosome XI.";
Nature 369:371-378(1994).
[2]
IDENTIFICATION, AND SUBCELLULAR LOCATION.
PubMed=9603875 [NCBI, ExPASy, EBI, Israel, Japan]
Boles E., de Jong-Gubbels P., Pronk J.T.;
"Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme.";
J. Bacteriol. 180:2875-2882(1998).
[3]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[4]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
DOI=10.1073/pnas.2135385100; PubMed=14576278 [NCBI, ExPASy, EBI, Israel, Japan]
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.;
"The proteome of Saccharomyces cerevisiae mitochondria.";
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z28029; CAA81865.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S37846; S37846.
RefSeq NP_012896.1; -.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase P36013.
Protein-protein interaction databases
DIP DIP:4444N; -.
IntAct P36013; -.
Organism-specific databases
CYGD YKL029c; -.
SGD S000001512; MAE1.
Yeast-GFP YKL029C.
Gene expression databases
ArrayExpress P36013; -.
GermOnline YKL029C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0004470; Molecular function: malic enzyme activity (inferred from direct assay from SGD).
GO:0006520; Biological process: amino acid metabolic process (inferred from mutant phenotype from SGD).
GO:0006090; Biological process: pyruvate metabolic process (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P36013.
Proteomic databases
PeptideAtlas P36013; -.
Genome annotation databases
Ensembl YKL029C; Saccharomyces cerevisiae. [Contig view]
GeneID 853839; -.
GenomeReviews Y13137_GR; YKL029C.
KEGG sce:YKL029C; -.
NMPDR fig|4932.3.peg.3881; -.
Phylogenomic databases
HOGENOM P36013; -.
Other
LinkHub P36013; -.
ProtoNet P36013.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion. 
CHAIN   ?   669        NAD-dependent malic enzyme, mitochondrial. PRO_0000160206
ACT_SITE   187   187        Proton donor (By similarity). 
ACT_SITE   259   259        Proton acceptor (By similarity). 
METAL   330   330        Divalent metal cation (By similarity). 
METAL   331   331        Divalent metal cation (By similarity). 
METAL   354   354        Divalent metal cation (By similarity). 
BINDING   354   354        NAD (By similarity). 
BINDING   499   499        NAD (By similarity). 
SITE   354   354  1     Important for activity (By similarity). 
Sequence information
Length: 669 AA [This is the length of the unprocessed precursor] Molecular weight: 74376 Da [This is the MW of the unprocessed precursor] CRC64: 0122B52E846B748F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLRTRLSVSV AARSQLTRSL TASRTAPLRR WPIQQSRLYS SNTRSHKATT TRENTFQKPY 

        70         80         90        100        110        120 
SDEEVTKTPV GSRARKIFEA PHPHATRLTV EGAIECPLES FQLLNSPLFN KGSAFTQEER 

       130        140        150        160        170        180 
EAFNLEALLP PQVNTLDEQL ERSYKQLCYL KTPLAKNDFM TSLRVQNKVL YFALIRRHIK 

       190        200        210        220        230        240 
ELVPIIYTPT EGDAIAAYSH RFRKPEGVFL DITEPDSIEC RLATYGGDKD VDYIVVSDSE 

       250        260        270        280        290        300 
GILGIGDQGI GGVRIAISKL ALMTLCGGIH PGRVLPVCLD VGTNNKKLAR DELYMGNKFS 

       310        320        330        340        350        360 
RIRGKQYDDF LEKFIKAVKK VYPSAVLHFE DFGVKNARRL LEKYRYELPS FNDDIQGTGA 

       370        380        390        400        410        420 
VVMASLIAAL KHTNRDLKDT RVLIYGAGSA GLGIADQIVN HMVTHGVDKE EARKKIFLMD 

       430        440        450        460        470        480 
RRGLILQSYE ANSTPAQHVY AKSDAEWAGI NTRSLHDVVE NVKPTCLVGC STQAGAFTQD 

       490        500        510        520        530        540 
VVEEMHKHNP RPIIFPLSNP TRLHEAVPAD LMKWTNNNAL VATGSPFPPV DGYRISENNN 

       550        560        570        580        590        600 
CYSFPGIGLG AVLSRATTIT DKMISAAVDQ LAELSPLREG DSRPGLLPGL DTITNTSARL 

       610        620        630        640        650        660 
ATAVILQALE EGTARIEQEQ VPGGAPGETV KVPRDFDECL QWVKAQMWEP VYRPMIKVQH 


DPSVHTNQL 

P36013 in FASTA format

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