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UniProtKB/Swiss-Prot entry P35688


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LRG1_YEAST
Primary accession number P35688
Secondary accession number Q07735
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    April 29, 2008 (Entry version 81)
Name and origin of the protein
Protein name Rho-GTPase-activating protein LRG1
Synonym LIM-RhoGAP protein 1
Gene name
Name: LRG1
OrderedLocusNames: YDL240W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DBY874;
DOI=10.1093/nar/22.15.3151; PubMed=8065929 [NCBI, ExPASy, EBI, Israel, Japan]
Mueller A., Xu G., Wells R., Hollenberg C.P., Piepersberg W.;
"LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains.";
Nucleic Acids Res. 22:3151-3154(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867 [NCBI, ExPASy, EBI, Israel, Japan]
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
[3]
FUNCTION, AND INTERACTION WITH CDC42; RHO1 AND RHO2.
DOI=10.1016/S0014-5793(01)02906-4; PubMed=11591390 [NCBI, ExPASy, EBI, Israel, Japan]
Roumanie O., Weinachter C., Larrieu I., Crouzet M., Doignon F.;
"Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae.";
FEBS Lett. 506:149-156(2001).
[4]
FUNCTION, INTERACTION WITH RHO1, AND SUBCELLULAR LOCATION.
DOI=10.1002/yea.742; PubMed=11447600 [NCBI, ExPASy, EBI, Israel, Japan]
Watanabe D., Abe M., Ohya Y.;
"Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis.";
Yeast 18:943-951(2001).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X78453; CAA55210.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z74288; CAA98820.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S67804; S67804.
RefSeq NP_010041.1; -.
3D structure databases
HSSP Q07960; 1RGP. [HSSP ENTRY / PDB]
ModBase P35688.
Protein-protein interaction databases
DIP DIP:2588N; -.
Organism-specific databases
CYGD YDL240w; -.
SGD S000002399; LRG1.
Yeast-GFP YDL240W.
Gene expression databases
ArrayExpress P35688; -.
GermOnline YDL240W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005935; Cellular component: cellular bud neck (inferred from direct assay from SGD).
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0005100; Molecular function: Rho GTPase activator activity (inferred from direct assay from SGD).
GO:0009272; Biological process: fungal-type cell wall biogenesis (inferred from genetic interaction from SGD).
GO:0007264; Biological process: small GTPase mediated signal transduction (inferred from physical interaction from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000198; RhoGAP.
IPR001781; Znf_LIM.
Graphical view of domain structure.
Gene3D G3DSA:1.10.555.10; RhoGAP; 1.
G3DSA:2.10.110.10; Znf_LIM; 2.
Pfam PF00412; LIM; 3.
PF00620; RhoGAP; 1.
Pfam graphical view of domain structure.
ProDom PD000094; LIM; 3.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00132; LIM; 3.
SM00324; RhoGAP; 1.
SMART graphical view of domain structure.
PROSITE PS00478; LIM_DOMAIN_1; 2.
PS50023; LIM_DOMAIN_2; 3.
PS50238; RHOGAP; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P35688.
Genome annotation databases
Ensembl YDL240W; Saccharomyces cerevisiae. [Contig view]
GeneID 851358; -.
GenomeReviews Z71256_GR; YDL240W.
KEGG sce:YDL240W; -.
NMPDR fig|4932.3.peg.774; -.
Other
LinkHub P35688; -.
ProtoNet P35688.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; GTPase activation; LIM domain; Metal-binding; Repeat; Sporulation; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   1017  1017     Rho-GTPase-activating protein LRG1. PRO_0000075838
DOMAIN   28     88  61     LIM zinc-binding 1. 
DOMAIN   98    148  51     LIM zinc-binding 2. 
DOMAIN   155    184  30     LIM zinc-binding 3; truncated. 
DOMAIN   419    474  56     LIM zinc-binding 4. 
DOMAIN   730    953  224     Rho-GAP. 
CONFLICT   531    531        H -> Q (in Ref. 1; CAA55210). 
CONFLICT   766    766        R -> S (in Ref. 1; CAA55210). 
CONFLICT   791    791        N -> T (in Ref. 1; CAA55210). 
CONFLICT   821    821        L -> Q (in Ref. 1; CAA55210). 
CONFLICT   838    838        A -> S (in Ref. 1; CAA55210). 
CONFLICT   849    849        V -> L (in Ref. 1; CAA55210). 
CONFLICT   895    895        S -> F (in Ref. 1; CAA55210). 
CONFLICT   928    928        A -> T (in Ref. 1; CAA55210). 
CONFLICT   935    935        Y -> S (in Ref. 1; CAA55210). 
CONFLICT   977   1017        INHFISTVMQSKTIDYSECDIKTPVTVKDSTTTVIQ GEINK -> TILFPPLCKVKQSIIPNVT (in Ref. 1; CAA55210). 
Sequence information
Length: 1017 AA [This is the length of the unprocessed precursor] Molecular weight: 116660 Da [This is the MW of the unprocessed precursor] CRC64: DDEB79B4BBEBBB09 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIQNSAGYRS LNTASPMTVQ VKNQKKICAR CNKLVIPDSQ RTKTTLKALG KYYHESCFTC 

        70         80         90        100        110        120 
QDCQKPLKPK YFPYQVDKTS ESILLCQYDY FRRHNLLCHV CDTPLRGLYY TAFGYRYDEE 

       130        140        150        160        170        180 
HFSCTICATP CGVKKCFMYG NQLYCKYHFL KYFSKRCKGC EFPISDQYIE FPKGEEIHCW 

       190        200        210        220        230        240 
HPECYGIHKY WHVNLAAETV GLQYLPKLEY NPNSGDKDIN PTAYELDKQM QAFNFILSKT 

       250        260        270        280        290        300 
WSVLYRFEEE AASCISDMFQ YLTSNDQLKG IESTGLLVLK IDCLFRGLDT LNLSTNKSMP 

       310        320        330        340        350        360 
VNSDQECIEN NAMAASKYSK FPKNLSTKIM IYLQLLRKLG TENKNETITI SSFMSVITGL 

       370        380        390        400        410        420 
AHFLKLLTRF GLYTALENNK LTHSVNPLLR FLREVEKNEL FENNPFQYIK TPVNATDSCA 

       430        440        450        460        470        480 
GCNKYIQEEC IQFYEHRWHI ACFTCSSCHK NINPRSLTDP TFNKEKKKIL CSHCSIDDPA 

       490        500        510        520        530        540 
SVPGFKFVTK LAQLIFLLKI ALVKSRTVML KSKASNKVGR NSLQSTMLKE HTYIRTLNDI 

       550        560        570        580        590        600 
KRLRSRRESV RVTHNKQQAR KSVILETAET DLNDPTKQGD SKNLVIQTDD PSSSQQVSTR 

       610        620        630        640        650        660 
ENVFSNTKTL TLDDISRIVA AEQARELRPN AFAHFKKLKE TDDETSNVVP KKSGVYYSEL 

       670        680        690        700        710        720 
STMELSMIRA ISLSLLAGKQ LISKTDPNYT SLVSMVFSNE KQVTGSFWNR MKIMMSMEPK 

       730        740        750        760        770        780 
KPITKTVFGA PLDVLCEKWG VDSDLGVGPV KIRIPIIIDE LISSLRQMDM SVEGIFRKNG 

       790        800        810        820        830        840 
NIRRLRELTA NIDSNPTEAP DFSKENAIQL SALLKKFIRE LPQPILSTDL YELWIKAAKI 

       850        860        870        880        890        900 
DLEDEKQRVI LLIYSLLPTY NRNLLEALLS FLHWTSSFSY IENEMGSKMD IHNLSTVITP 

       910        920        930        940        950        960 
NILYLRHKEI SNDNVPDEPE SGLVDSFAQN KGENYFLAIE IVDYLITHNE EMAMVPKFLM 

       970        980        990       1000       1010 
NLLKDVQLQK LDNYESINHF ISTVMQSKTI DYSECDIKTP VTVKDSTTTV IQGEINK 

P35688 in FASTA format

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