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UniProtKB/Swiss-Prot entry P35401


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CRAC_DICDI
Primary accession number P35401
Secondary accession number Q54NJ7
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on December 4, 2007 (Sequence version 4)
Annotations were last modified on    September 23, 2008 (Entry version 40)
Name and origin of the protein
Protein name Protein CRAC
Synonyms None
Gene name
Name: dagA
ORFNames: DDB_0191434
From
Dictyostelium discoideum (Slime mold) [TaxID: 44689] 
Taxonomy Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=AX4;
DOI=10.1083/jcb.126.6.1537; PubMed=8089184 [NCBI, ExPASy, EBI, Israel, Japan]
Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F., Devreotes P.N.;
"CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium.";
J. Cell Biol. 126:1537-1545(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=AX4;
DOI=10.1038/nature03481; PubMed=15875012 [NCBI, ExPASy, EBI, Israel, Japan]
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
"The genome of the social amoeba Dictyostelium discoideum.";
Nature 435:43-57(2005).
[3]
PROTEIN SEQUENCE OF 2-9.
PubMed=8188693 [NCBI, ExPASy, EBI, Israel, Japan]
Lilly P.J., Devreotes P.N.;
"Identification of CRAC, a cytosolic regulator required for guanine nucleotide stimulation of adenylyl cyclase in Dictyostelium.";
J. Biol. Chem. 269:14123-14129(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U06228; AAA61782.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AAFI02000075; EAL64837.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A54796; A54796.
RefSeq XP_638365.1; -.
3D structure databases
ModBase P35401.
Organism-specific databases
dictyBase DDB0191434; dagA.
Family and domain databases
InterPro IPR001849; PH.
IPR011993; PH_type.
Graphical view of domain structure.
Gene3D G3DSA:2.30.29.30; PH_type; 1.
Pfam PF00169; PH; 1.
Pfam graphical view of domain structure.
SMART SM00233; PH; 1.
SMART graphical view of domain structure.
PROSITE PS50003; PH_DOMAIN; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P35401.
Genome annotation databases
GeneID 3389921; -.
KEGG ddi:DDB_0191434; -.
Other
ProtoNet P35401.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Direct protein sequencing.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   698  697     Protein CRAC. PRO_0000079325
DOMAIN   22   122  101     PH. 
COMPBIAS   350   363  14     Poly-Thr. 
COMPBIAS   468   480  13     Poly-Gly. 
COMPBIAS   613   624  12     Poly-Ser. 
CONFLICT   653   653        R -> G (in Ref. 1; AAA61782). 
Sequence information
Length: 698 AA [This is the length of the unprocessed precursor] Molecular weight: 78630 Da [This is the MW of the unprocessed precursor] CRC64: 3CC3D8FF6D9D849B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY ILYYKLGKSS 

        70         80         90        100        110        120 
LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY IVKAKDESSM KQFLTLLIAR 

       130        140        150        160        170        180 
IRSLEKIDID KLGCTVVVLT KVKKFREVLT NPLILPDRVS PEMAEEWVKQ MKNYNASFNL 

       190        200        210        220        230        240 
ADPFIKQVEQ ISEFCRGEVK EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT 

       250        260        270        280        290        300 
YQDNRFFRED FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK 

       310        320        330        340        350        360 
FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT STTTTTTTTT 

       370        380        390        400        410        420 
TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS PHHQHLHHQI VSNSKDFNIS 

       430        440        450        460        470        480 
VSSNNFNDGN SEFPNLDINC DFDLTSASNL SSPILSSEVP SNVVDPIGSG QGGGGSGGGG 

       490        500        510        520        530        540 
VTAVTEEAIN EKWHFDCNTS MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY 

       550        560        570        580        590        600 
QCGYFKSKNM GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP 

       610        620        630        640        650        660 
SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII EGRHQPSLTL 

       670        680        690 
IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK 

P35401 in FASTA format

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