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UniProtKB/Swiss-Prot entry P35262

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_MABVP
Primary accession number P35262
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on February 1, 1994 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 52)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)) [TaxID: 33728] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae; Marburgvirus.
Virus hosts Cercopithecus aethiops (Green monkey) (Grivet) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1007/BF01322532; PubMed=7487490 [NCBI, ExPASy, EBI, Israel, Japan]
Bukreyev A.A., Volchkov V.E., Blinov V.M., Dryga S.A., Netesov S.V.;
"The complete nucleotide sequence of the Popp (1967) strain of Marburg virus: a comparison with the Musoke (1980) strain.";
Arch. Virol. 140:1589-1600(1995).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBCELLULAR LOCATION: Cytoplasm. Virion (By similarity).
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X68494; CAA48508.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z29337; CAA82542.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S44054; S44054.
3D structure databases
ModBase P35262.
Family and domain databases
InterPro IPR017235; RNA-dir_RNA_pol_filovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037548; RNA_pol_Filoviridae; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P35262.
Other
ProtoNet P35262.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   2331  2331     Large structural protein. PRO_0000222170
DOMAIN   628    812  185     RdRp catalytic. 
Sequence information
Length: 2331 AA [This is the length of the unprocessed precursor] Molecular weight: 266638 Da [This is the MW of the unprocessed precursor] CRC64: 7ED1D61D0EEF9B8B [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MQHPTQYPDA RLSSPIILDQ CDLLARSLGL YSHYSHNPKL RNCRIPHHIY RLRNSTALKT 

        70         80         90        100        110        120 
FLQNCSILTV PFHSIWDHIL TSIQYDAINH VDDFKYLLPS ELVKYANWDN EFLKAYLNKI 

       130        140        150        160        170        180 
LGLDHVFPAS ARSQWEDFSP KENPYYWGML LLVHLSQLAR RIKGQRGSLR SNWKFIGTDL 

       190        200        210        220        230        240 
ELFGIADFII FKVPVKTIIR NAVSLQASKP GLRVWYRDQN LTPYLCDDEF IVSVASYECF 

       250        260        270        280        290        300 
IMIKDVFIER YNTWEICARA WLEDSDGADY LPLDVLGELY NQGDQIIAMY LEDGFKLIKH 

       310        320        330        340        350        360 
LEPLCVSCIQ THGIFTPGKY WFQSQRIESY YEELCSLNWK FKISGNKAEC AQNFIKTIIQ 

       370        380        390        400        410        420 
GKLTPQQYCE LFSLQKHWGH PVLYIDVALD KVKKHAQSVK ILKPKVMFET FCVFKFIVAK 

       430        440        450        460        470        480 
NHYHSQGSWY KTTMDLHLTP YLRQHIVSNS FPSQAEIYQH LWEWYFVEHE PLFSTKIISD 

       490        500        510        520        530        540 
LSIFIKDRAT AVNQECWDSV FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILDFAEK 

       550        560        570        580        590        600 
LEYLAPSYRN FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT 

       610        620        630        640        650        660 
EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH FIDYCNRCYG 

       670        680        690        700        710        720 
VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT EDNRNNPPDC ANAYHYHLGG IEGLQQKLWT 

       730        740        750        760        770        780 
CISCAQITLV ELKTKLKLKS SVMGDNQCIT TLSLFPIDAP DDYQENEAEL NAARVAVELA 

       790        800        810        820        830        840 
ITTGYDGIFL KPEETFVHSG FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL 

       850        860        870        880        890        900 
ASIGTSFERG TSETRHIFPS RWIASFHSML AINLLNQNHL GFPLGFSIDI SCFKKPLTFS 

       910        920        930        940        950        960 
EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLK NALEFLEKEE LFYILIAKKP 

       970        980        990       1000       1010       1020 
GLADASDFVM NPLGLNVPGS REIITFLRQT VRENITITSQ NRIINSLFHI GSDLEDQRVC 

      1030       1040       1050       1060       1070       1080 
EWLLSSNPVM SRFAADIFSR TPSGKRLQVL GYLEGTRTLL ASRTISLTTE GTMLMKLREL 

      1090       1100       1110       1120       1130       1140 
TRNRWKSWFS YIDALDDDLS ESLEKFTCTV DIANFLRAYS WLDVLKGKRL IGATLPCLLE 

      1150       1160       1170       1180       1190       1200 
QFKVKWINLS EDLREQFNMS SESESTINLL PYDCKELRLG RSNDTELNYV SCALDRKVVQ 

      1210       1220       1230       1240       1250       1260 
KHPSVNRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR LLWVTQGTAD 

      1270       1280       1290       1300       1310       1320 
REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY GQHSFMANRM SNTSTRAIIS 

      1330       1340       1350       1360       1370       1380 
TNTLGKYAGG GQAAVDSNII FQNTINLGVA VLDIALSLAK LSSASNVTFR LMLNKCCTRH 

      1390       1400       1410       1420       1430       1440 
VPSEYLFFDK PLDVDLNKYM DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD 

      1450       1460       1470       1480       1490       1500 
FPTIAAWTLG ETIVGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE 

      1510       1520       1530       1540       1550       1560 
SLSLSRIKSI KNLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH HIPLISLMLG 

      1570       1580       1590       1600       1610       1620 
GSAGEKSSSD AVRLFLTASY QNFINNFSCL MKKGQSSLPV WLYFPSEGQQ LKPILKILQR 

      1630       1640       1650       1660       1670       1680 
LSDLLSPDKV QKHQILADTC CPIDSFWVYP SKSTRTNHYY ASLNYWRDKA NKVKNTPFSH 

      1690       1700       1710       1720       1730       1740 
LINCSFLELS SHTSSVSSNQ QVTNSKYIVH PENIPEINAR TKLIDYGSTA LQGMDIKMPL 

      1750       1760       1770       1780       1790       1800 
SEQNLVGNCR PSKGIRFKDN PKTTKHDQGF VGKDSSPRPM SPEDNMQTPA YIHSSPPYQT 

      1810       1820       1830       1840       1850       1860 
LTKSPDVHED FDASKVILNS EINNLNLTDC TLNTKSLTTP TGTEILGISP FRSSRYSSTS 

      1870       1880       1890       1900       1910       1920 
RERSRLSREQ ASYLYVDCSN IPSISLDPGF QNMSDQNQVQ MLINTYKRDL HACFDSNQFC 

      1930       1940       1950       1960       1970       1980 
RFTGVVSSMH YKLYDLLPPG ELRKAICLAE GEGSGARLLL KWKKTDYLFF NTLATDSQQE 

      1990       2000       2010       2020       2030       2040 
AEILSGRVIP RMLYNIDRLN ALLESRRLIL NNLTIQITDI TSPLWLDSVI QYLPEDSDIL 

      2050       2060       2070       2080       2090       2100 
TMDAETTKDE TREQLYKTIV NIWTRTSPNI PKISIIKVFL LDYEGTLFLM RNAIQYYGQV 

      2110       2120       2130       2140       2150       2160 
QLKKPYSSNA KNSEWYLCCG KRRIQRLKID FSDQVGIFLI CKAMSRQRQA IPYWLKHIEK 

      2170       2180       2190       2200       2210       2220 
NYPASLHKFF LTLGFPSLES SFCHRYTIPF SEGKALFHKV QSYVRQGKQH LHSLMLDYEN 

      2230       2240       2250       2260       2270       2280 
NSPLLDLRNH FICSLRGKIT KYYNDILKLN LVIKAVEKGK NWSQLVETLP NMHSVCIVHV 

      2290       2300       2310       2320       2330 
DHECFGCEKR LLLKLDFIRN TKIAEQKLLN RVIGYILFFP FGLFKSESLT A
P35262 in FASTA format

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