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UniProtKB/Swiss-Prot entry P35244


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RFA3_HUMAN
Primary accession number P35244
Secondary accession number Q549U6
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on February 1, 1994 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 82)
Name and origin of the protein
Protein name Replication protein A 14 kDa subunit
Synonyms RP-A p14
Replication factor A protein 3
RF-A protein 3
Gene name
Name: RPA3
Synonyms: REPA3, RPA14
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=8454588 [NCBI, ExPASy, EBI, Israel, Japan]
Umbricht C.B., Kelly T.J.;
"Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A.";
J. Biol. Chem. 268:6131-6138(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
NIEHS SNPs program;
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature01782; PubMed=12853948 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.;
"The DNA sequence of human chromosome 7.";
Nature 424:157-164(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Kidney, and Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
INTERACTION WITH RPA4.
PubMed=7760808 [NCBI, ExPASy, EBI, Israel, Japan]
Keshav K.F., Chen C., Dutta A.;
"Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex.";
Mol. Cell. Biol. 15:3119-3128(1995).
[7]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
[8]
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RPA1 AND RPA2.
DOI=10.1093/emboj/21.7.1855; PubMed=11927569 [NCBI, ExPASy, EBI, Israel, Japan]
Bochkareva E., Korolev S., Lees-Miller S.P., Bochkarev A.;
"Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA.";
EMBO J. 21:1855-1863(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L07493; AAA58350.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007320; AAP35984.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ003136; AAX84517.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC004948; AAQ96878.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC005264; AAH05264.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC009868; AAH09868.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00017373; -.
PIR A46008; A46008.
RefSeq NP_002938.1; -.
UniGene Hs.487540
3D structure databases
PDB
1L1O; X-ray; 2.80 A; A/D=1-121.[ExPASy / RCSB / EBI]
1QUQ; X-ray; 2.50 A; B/D=1-121.[ExPASy / RCSB / EBI]
2PI2; X-ray; 2.00 A; E/F/G/H=1-121.[ExPASy / RCSB / EBI]
2PQA; X-ray; 2.50 A; B/D=1-121.[ExPASy / RCSB / EBI]
2Z6K; X-ray; 3.00 A; C/D=1-121.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1L1O; -.
1QUQ; -.
2PI2; -.
2PQA; -.
2Z6K; -.
ModBase P35244.
Protein-protein interaction databases
DIP DIP:24190N; -.
IntAct P35244; 3.
Enzyme and pathway databases
Reactome REACT_152; Cell Cycle, Mitotic.
REACT_1538; Cell Cycle Checkpoints.
REACT_216; DNA Repair.
REACT_383; DNA Replication.
REACT_7970; Telomere Maintenance.
Organism-specific databases
GeneCards GC07M007643; -.
H-InvDB HIX0017394; -.
HIX0057108; -.
HGNC HGNC:10291; RPA3.
GenAtlas RPA3.
HPA HPA005708; -.
MIM 179837; gene. [NCBI / EBI]
PharmGKB PA29357; -.
Gene expression databases
ArrayExpress P35244; -.
Bgee P35244; -.
CleanEx HS_RPA3; -.
GermOnline ENSG00000106399; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0005662; Cellular component: DNA replication factor A complex (traceable author statement from ProtInc).
GO:0005654; Cellular component: nucleoplasm (inferred from experiment from Reactome).
GO:0003697; Molecular function: single-stranded DNA binding (traceable author statement from ProtInc).
GO:0006260; Biological process: DNA replication (inferred from experiment from Reactome).
GO:0000718; Biological process: nucleotide-excision repair, DNA damage removal (inferred from experiment from Reactome).
GO:0006297; Biological process: nucleotide-excision repair, DNA gap filling (inferred from experiment from Reactome).
QuickGo view.
Family and domain databases
InterPro IPR012340; NA-bd_OB-fold.
IPR013970; Rep_factor-A_3.
Graphical view of domain structure.
Gene3D G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
Pfam PF08661; Rep_fac-A_3; 1.
Pfam graphical view of domain structure.
Proteomic databases
PeptideAtlas P35244; -.
PRIDE P35244; -.
Genome annotation databases
Ensembl ENSG00000106399; Homo sapiens. [Contig view]
GeneID 6119; -.
KEGG hsa:6119; -.
Phylogenomic databases
HOGENOM P35244; -.
HOVERGEN P35244; -.
OMA P35244; TIMCASY.
Other
NextBio 23763; -.
SOURCE RPA3; Homo sapiens.
ProtoNet P35244.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; DNA replication; Nucleus.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   121  121     Replication protein A 14 kDa subunit. PRO_0000097276
HELIX   4     6  3      
STRAND   10    12  3      
HELIX   14    19  6      
STRAND   24    34  11      
STRAND   38    44  7      
STRAND   50    54  5      
STRAND   65    73  9      
STRAND   79    86  8      
STRAND   90    92  3      
HELIX   96   108  13      
HELIX   110   112  3      
Sequence information
Length: 121 AA [This is the length of the unprocessed precursor] Molecular weight: 13569 Da [This is the MW of the unprocessed precursor] CRC64: 3FD99851959FB498 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVDMMDLPRS RINAGMLAQF IDKPVCFVGR LEKIHPTGKM FILSDGEGKN GTIELMEPLD 

        70         80         90        100        110        120 
EEISGIVEVV GRVTAKATIL CTSYVQFKED SHPFDLGLYN EAVKIIHDFP QFYPLGIVQH 


D 

P35244 in FASTA format

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