[1]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=cv. Excalibur; TISSUE=Cotyledon; DOI=10.1104/pp.102.3.1049; PubMed=8278526 [NCBI, ExPASy, EBI, Israel, Japan] Pramanik S.K., Bewley J.D.; "A ubiquitin carrier protein cDNA from developing alfalfa embryos."; Plant Physiol. 102:1049-1050(1993).

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity).
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L06967; AAA18528.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P25865; 2AAK. [HSSP ENTRY / PDB]

SMR

P35130; 1-150.

ModBase

P35130.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Ligase; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 152`	`152`	`Ubiquitin-conjugating enzyme E2 2.`	`PRO_0000082574`
`ACT_SITE`	`88 88`		`Glycyl thioester intermediate (By similarity).`

Sequence information

Length: 152 AA [This is the length of the unprocessed precursor]

Molecular weight: 17315 Da [This is the MW of the unprocessed precursor]

CRC64: 3951D40CF5CE8E24 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTPARKRLM RDFKRLQHDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG GTFKLSLQFS 

        70         80         90        100        110        120 
EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAILTSIQ SLLCDPNPNS 

       130        140        150 
PANSEAARMF SENKREYNRR VREVVEQSWT AD

P35130 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools