[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=8441382 [NCBI, ExPASy, EBI, Israel, Japan] Zhen M., Heinlein R., Jones D., Jentsch S., Candido E.P.M.; "The ubc-2 gene of Caenorhabditis elegans encodes a ubiquitin-conjugating enzyme involved in selective protein degradation."; Mol. Cell. Biol. 13:1371-1377(1993).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION AS LET-70. PubMed=8670823 [NCBI, ExPASy, EBI, Israel, Japan] Zhen M., Scheni J.E., Baillie D.L., Candido E.P.M.; "An essential ubiquitin-conjugating enzyme with tissue and developmental specificity in the nematode Caenorhabditis elegans."; EMBO J. 15:3229-3237(1996).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Bristol N2; DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan] The C. elegans sequencing consortium; "Genome sequence of the nematode C. elegans: a platform for investigating biology."; Science 282:2012-2018(1998).

Comments

FUNCTION: Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
PATHWAY: Protein modification; protein ubiquitination .
INTERACTION:
Q9XVR6:-; NbExp=1; IntAct=EBI-316050, EBI-316044;
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S56051; AAB25489.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z68337; CAA92745.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A48145; A48145.
T23820; T23820.

RefSeq

NP_502065.1; -.

UniGene

Cel.18192

3D structure databases

PDB

1Z2U; X-ray; 1.10 A; A=1-147.

[ExPASy / RCSB / EBI]

PDBsum

1Z2U; -.

ModBase

P35129.

Protein-protein interaction databases

DIP

DIP:24306N; -.

IntAct

P35129; -.

Organism-specific databases

WormBase

WBGene00002344; let-70.

WormPep

M7.1; CE03482. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

P35129; -.

Ontologies

GO:0031625;	Molecular function: ubiquitin protein ligase binding (inferred from physical interaction from WormBase).
GO:0008340;	Biological process: determination of adult life span (inferred from mutant phenotype from WormBase).
GO:0009792;	Biological process: embryonic development ending in birth or egg hatching (inferred from mutant phenotype from WormBase).
GO:0040007;	Biological process: growth (inferred from mutant phenotype from WormBase).
GO:0040035;	Biological process: hermaphrodite genitalia development (inferred from mutant phenotype from WormBase).
GO:0040011;	Biological process: locomotion (inferred from mutant phenotype from WormBase).
GO:0002119;	Biological process: nematode larval development (inferred from mutant phenotype from WormBase).
QuickGo view.

Family and domain databases

InterPro

IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.

PANTHER

PTHR11621; UBQ-conjugat_E2; 1.

Pfam

PF00179; UQ_con; 1.
Pfam graphical view of domain structure.

ProDom

PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00212; UBCc; 1.
SMART graphical view of domain structure.

PROSITE

PS00183; UBIQUITIN_CONJUGAT_1; 1.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P35129.

Genome annotation databases

Ensembl

M7.1; Caenorhabditis elegans. [Contig view]

GeneID

178006; -.

KEGG

cel:M7.1; -.

NMPDR

fig|6239.3.peg.15092; -.

Other

ProtoNet

P35129.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Complete proteome; Ligase; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 147`	`147`	`Ubiquitin-conjugating enzyme E2 2.`	`PRO_0000082515`
`ACT_SITE`	`85 85`		`Glycyl thioester intermediate (By similarity).`
`VARIANT`	`75 75`	`1`	`H -> Y (in let-70(S1132); which shows growth arrest in larval stage).`

Sequence information

Length: 147 AA [This is the length of the unprocessed precursor]

Molecular weight: 16705 Da [This is the MW of the unprocessed precursor]

CRC64: 9E91884D307F2120 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALKRIQKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PESPYQGGVF FLTIHFPTDY 

        70         80         90        100        110        120 
PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV 

       130        140 
PEIARIYKTD RERYNQLARE WTQKYAM

P35129 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools