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UniProtKB/Swiss-Prot entry P32816

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GLDA_BACST
Primary accession number P32816
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 55)
Name and origin of the protein
Protein name Glycerol dehydrogenase
Synonyms GLDH
EC 1.1.1.6
Gene name
Name: gldA
Synonyms: gld
From
Bacillus stearothermophilus (Geobacillus stearothermophilus) [TaxID: 1422] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 2334 / Var. Non-diastaticus;
DOI=10.1016/0378-1119(92)90438-U; PubMed=1339360 [NCBI, ExPASy, EBI, Israel, Japan]
Mallinder P.R., Pritchard A., Moir A.;
"Cloning and characterization of a gene from Bacillus stearothermophilus var. non-diastaticus encoding a glycerol dehydrogenase.";
Gene 110:9-16(1992).
[2]
 PROTEIN SEQUENCE OF 84-103, AND MUTAGENESIS OF LYS-97.
DOI=10.1016/0167-4838(93)90010-O; PubMed=8399385 [NCBI, ExPASy, EBI, Israel, Japan]
Paine L.J., Perry N., Popplewell A.G., Gore M.G., Atkinson T.;
"The identification of a lysine residue reactive to pyridoxal-5-phosphate in the glycerol dehydrogenase from the thermophile Bacillus stearothermophilus.";
Biochim. Biophys. Acta 1202:235-243(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M65289; AAA22477.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JQ1474; JQ1474.
3D structure databases
PDB
1JPU; X-ray; 1.80 A; A=1-370.[ExPASy / RCSB / EBI]
1JQ5; X-ray; 1.70 A; A=1-370.[ExPASy / RCSB / EBI]
1JQA; X-ray; 2.05 A; A=1-370.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1JPU; -.
1JQ5; -.
1JQA; -.
ModBase P32816.
Ontologies
GO
GO:0008888; Molecular function: glycerol dehydrogenase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR001670; Fe_AlcDHase.
IPR016205; Glycerol_DH.
Graphical view of domain structure.
Pfam PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000112; Glycerol_dehydrogenase; 1.
PROSITE PS00913; ADH_IRON_1; 1.
PS00060; ADH_IRON_2; 1.
BLOCKS P32816.
Other
ProtoNet P32816.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; Glycerol metabolism; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   370  370     Glycerol dehydrogenase. PRO_0000087826
MUTAGEN   97    97        K->H: Loss of activity. 
STRAND   5     8  4      
STRAND   11    16  6      
HELIX   19    22  4      
HELIX   23    27  5      
TURN   28    30  3      
STRAND   32    38  7      
HELIX   40    45  6      
HELIX   47    56  10      
STRAND   60    65  6      
HELIX   72    85  14      
STRAND   88    95  8      
HELIX   96   109  14      
STRAND   112   119  8      
STRAND   128   130  3      
STRAND   150   155  6      
HELIX   156   160  5      
HELIX   164   187  24      
STRAND   194   196  3      
HELIX   199   224  26      
HELIX   230   250  21      
HELIX   254   261  8      
HELIX   262   264  3      
HELIX   268   271  4      
HELIX   274   288  15      
HELIX   293   305  13      
TURN   312   316  5      
HELIX   322   332  11      
HELIX   338   341  4      
HELIX   347   365  19      
Sequence information
Length: 370 AA [This is the length of the unprocessed precursor] Molecular weight: 39501 Da [This is the MW of the unprocessed precursor] CRC64: E5D93264B1670F25 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAAERVFISP AKYVQGKNVI TKIANYLEGI GNKTVVIADE IVWKIAGHTI VNELKKGNIA 

        70         80         90        100        110        120 
AEEVVFSGEA SRNEVERIAN IARKAEAAIV IGVGGGKTLD TAKAVADELD AYIVIVPTAA 

       130        140        150        160        170        180 
STDAPTSALS VIYSDDGVFE SYRFYKKNPD LVLVDTKIIA NAPPRLLASG IADALATWVE 

       190        200        210        220        230        240 
ARSVIKSGGK TMAGGIPTIA AEAIAEKCEQ TLFKYGKLAY ESVKAKVVTP ALEAVVEANT 

       250        260        270        280        290        300 
LLSGLGFESG GLAAAHAIHN GFTALEGEIH HLTHGEKVAF GTLVQLALEE HSQQEIERYI 

       310        320        330        340        350        360 
ELYLSLDLPV TLEDIKLKDA SREDILKVAK AATAEGETIH NAFNVTADDV ADAIFAADQY 

       370 
AKAYKEKHRK
P32816 in FASTA format

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