ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P32477


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name GSH1_YEAST
Primary accession number P32477
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 67)
Name and origin of the protein
Protein name Glutamate--cysteine ligase
Synonyms EC 6.3.2.2
Gamma-glutamylcysteine synthetase
Gamma-ECS
GCS
Gene name
Name: GSH1
OrderedLocusNames: YJL101C
ORFNames: J0832
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1687097 [NCBI, ExPASy, EBI, Israel, Japan]
Ohtake Y., Yabuuchi S.;
"Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae.";
Yeast 7:953-961(1991).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/BF00312627; PubMed=8100487 [NCBI, ExPASy, EBI, Israel, Japan]
Lisowsky T.;
"A high copy number of yeast gamma-glutamylcysteine synthetase suppresses a nuclear mutation affecting mitochondrial translation.";
Curr. Genet. 23:408-413(1993).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=7483851 [NCBI, ExPASy, EBI, Israel, Japan]
Rasmussen S.W.;
"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg).";
Yeast 11:873-883(1995).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=8641269 [NCBI, ExPASy, EBI, Israel, Japan]
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
EMBO J. 15:2031-2049(1996).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D90220; BAA14251.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z17312; CAA78960.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X85021; CAA59393.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z49376; CAA89396.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S28648; S28648.
RefSeq NP_012434.1; -.
3D structure databases
ModBase P32477.
Organism-specific databases
CYGD YJL101c; -.
SGD S000003637; GSH1.
Yeast-GFP YJL101C.
Gene expression databases
ArrayExpress P32477; -.
GermOnline YJL101C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005622; Cellular component: intracellular (inferred from direct assay from SGD).
GO:0004357; Molecular function: glutamate-cysteine ligase activity (inferred from electronic annotation from InterPro).
GO:0006750; Biological process: glutathione biosynthetic process (inferred from electronic annotation from InterPro).
GO:0046686; Biological process: response to cadmium ion (inferred from direct assay from SGD).
GO:0009409; Biological process: response to cold (inferred from expression pattern from SGD).
GO:0042493; Biological process: response to drug (inferred from mutant phenotype from SGD).
GO:0009408; Biological process: response to heat (inferred from expression pattern from SGD).
GO:0042542; Biological process: response to hydrogen peroxide (inferred from mutant phenotype from SGD).
GO:0046689; Biological process: response to mercury ion (inferred from expression pattern from SGD).
QuickGo view.
Family and domain databases
InterPro IPR004308; GCS.
Graphical view of domain structure.
PANTHER PTHR11164; GCS; 1.
Pfam PF03074; GCS; 1.
Pfam graphical view of domain structure.
BLOCKS P32477.
ProtoNet P32477.
Proteomic databases
PeptideAtlas P32477; -.
Genome annotation databases
Ensembl YJL101C; Saccharomyces cerevisiae. [Contig view]
GeneID 853344; -.
GenomeReviews Y13136_GR; YJL101C.
KEGG sce:YJL101C; -.
NMPDR fig|4932.3.peg.3403; -.
Phylogenomic databases
HOGENOM P32477; -.
Other
LinkHub P32477; -.
NextBio 973735; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glutathione biosynthesis; Ligase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   678  678     Glutamate--cysteine ligase. PRO_0000192572
MOD_RES   116   116        Phosphoserine. 
CONFLICT   299   299        A -> R (in Ref. 2; CAA78960). 
CONFLICT   492   492        L -> V (in Ref. 2; CAA78960). 
CONFLICT   526   527        IL -> MV (in Ref. 2; CAA78960). 
CONFLICT   542   542        D -> V (in Ref. 2; CAA78960). 
CONFLICT   550   550        I -> M (in Ref. 2; CAA78960). 
Sequence information
Length: 678 AA [This is the length of the unprocessed precursor] Molecular weight: 78254 Da [This is the MW of the unprocessed precursor] CRC64: 65A345988A0C5AAC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGLLALGTPL QWFESRTYNE HIRDEGIEQL LYIFQAAGKR DNDPLFWGDE LEYMVVDFDD 

        70         80         90        100        110        120 
KERNSMLDVC HDKILTELNM EDSSLCEAND VSFHPEYGRY MLEATPASPY LNYVGSYVEV 

       130        140        150        160        170        180 
NMQKRRAIAE YKLSEYARQD SKNNLHVGSR SVPLTLTVFP RMGCPDFINI KDPWNHKNAA 

       190        200        210        220        230        240 
SRSLFLPDEV INRHVRFPNL TASIRTRRGE KVCMNVPMYK DIATPETDDS IYDRDWFLPE 

       250        260        270        280        290        300 
DKEAKLASKP GFIYMDSMGF GMGCSCLQVT FQAPNINKAR YLYDALVNFA PIMLAFSAAA 

       310        320        330        340        350        360 
PAFKGWLADQ DVRWNVISGA VDDRTPKERG VAPLLPKYNK NGFGGIAKDV QDKVLEIPKS 

       370        380        390        400        410        420 
RYSSVDLFLG GSKFFNRTYN DTNVPINEKV LGRLLENDKA PLDYDLAKHF AHLYIRDPVS 

       430        440        450        460        470        480 
TFEELLNQDN KTSSNHFENI QSTNWQTLRF KPPTQQATPD KKDSPGWRVE FRPFEVQLLD 

       490        500        510        520        530        540 
FENAAYSVLI YLIVDSILTF SDNINAYIHM SKVWENMKIA HHRDAILFEK FHWKKSFRND 

       550        560        570        580        590        600 
TDVETEDYSI SEIFHNPENG IFPQFVTPIL CQKGFVTKDW KELKHSSKHE RLYYYLKLIS 

       610        620        630        640        650        660 
DRASGELPTT AKFFRNFVLQ HPDYKHDSKI SKSINYDLLS TCDRLTHLDD SKGELTSFLG 

       670 
AEIAEYVKKN KPSIESKC 

P32477 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by au flag APAF Australia Mirror sites: Brazil  Canada  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!