ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P32397


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PPOX_BACSU
Primary accession number P32397
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 68)
Name and origin of the protein
Protein name Protoporphyrinogen oxidase
Synonyms PPO
EC 1.3.3.4
Gene name
Name: hemY
Synonyms: hemG
OrderedLocusNames: BSU10140
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1459957 [NCBI, ExPASy, EBI, Israel, Japan]
Hansson M., Hederstedt L.;
"Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.";
J. Bacteriol. 174:8081-8093(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=9579061 [NCBI, ExPASy, EBI, Israel, Japan]
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.;
"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene.";
Microbiology 144:859-875(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[4]
CHARACTERIZATION.
PubMed=8288631 [NCBI, ExPASy, EBI, Israel, Japan]
Dailey T.A., Meissner P., Dailey H.A.;
"Expression of a cloned protoporphyrinogen oxidase.";
J. Biol. Chem. 269:813-815(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M97208; AAA22519.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y14083; CAA74520.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99109; CAB12854.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D47045; D47045.
RefSeq NP_388895.1; -.
3D structure databases
ModBase P32397.
Enzyme and pathway databases
BioCyc BSUB224308:BSU1014-MON; -.
Organism-specific databases
SubtiList BG10431; hemY. [Micado]
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0004729; Molecular function: protoporphyrinogen oxidase activity (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006779; Biological process: porphyrin biosynthetic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002937; Amino_oxidase.
IPR004572; Proto_IX_ox.
Graphical view of domain structure.
Pfam PF01593; Amino_oxidase; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00562; proto_IX_ox; 1.
BLOCKS P32397.
ProtoNet P32397.
Genome annotation databases
GeneID 936311; -.
GenomeReviews AL009126_GR; BSU10140.
KEGG bsu:BSU10140; -.
NMPDR fig|224308.1.peg.1014; -.
Phylogenomic databases
HOGENOM P32397; -.
Genome annotation databases
CMR P32397; BSU10140.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Porphyrin biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   470  470     Protoporphyrinogen oxidase. PRO_0000135263
NP_BIND   12    17  6     FAD (Potential). 
Sequence information
Length: 470 AA [This is the length of the unprocessed precursor] Molecular weight: 51203 Da [This is the MW of the unprocessed precursor] CRC64: 95CC4E5847686D2E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSDGKKHVVI IGGGITGLAA AFYMEKEIKE KNLPLELTLV EASPRVGGKI QTVKKDGYII 

        70         80         90        100        110        120 
ERGPDSFLER KKSAPQLVKD LGLEHLLVNN ATGQSYVLVN RTLHPMPKGA VMGIPTKIAP 

       130        140        150        160        170        180 
FVSTGLFSLS GKARAAMDFI LPASKTKDDQ SLGEFFRRRV GDEVVENLIE PLLSGIYAGD 

       190        200        210        220        230        240 
IDKLSLMSTF PQFYQTEQKH RSLILGMKKT RPQGSGQQLT AKKQGQFQTL STGLQTLVEE 

       250        260        270        280        290        300 
IEKQLKLTKV YKGTKVTKLS HSGSCYSLEL DNGVTLDADS VIVTAPHKAA AGMLSELPAI 

       310        320        330        340        350        360 
SHLKNMHSTS VANVALGFPE GSVQMEHEGT GFVISRNSDF AITACTWTNK KWPHAAPEGK 

       370        380        390        400        410        420 
TLLRAYVGKA GDESIVDLSD NDIINIVLED LKKVMNINGE PEMTCVTRWH ESMPQYHVGH 

       430        440        450        460        470 
KQRIKELREA LASAYPGVYM TGASFEGVGI PDCIDQGKAA VSDALTYLFS 

P32397 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by au flag APAF Australia Mirror sites: Brazil  Canada  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!