[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=S288c / GRF88; PubMed=8256521 [NCBI, ExPASy, EBI, Israel, Japan] Roennow B., Kielland-Brandt M.C.; "GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae."; Yeast 9:1121-1130(1993).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=ATCC 204511 / S288c / AB972; PubMed=9169870 [NCBI, ExPASy, EBI, Israel, Japan] Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.; "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."; Nature 387:84-87(1997).
[3]	SUBCELLULAR LOCATION. DOI=10.1021/bi010277r; PubMed=11502169 [NCBI, ExPASy, EBI, Israel, Japan] Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.; "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."; Biochemistry 40:9758-9769(2001).
[4]	LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan] Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.; "Global analysis of protein expression in yeast."; Nature 425:737-741(2003).

Comments

CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor.
COFACTOR: FAD.
PATHWAY: Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1 .
SUBCELLULAR LOCATION: Mitochondrion inner membrane.
MISCELLANEOUS: Present with 1670 molecules/cell in log phase SD medium.
SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z38059; CAA86123.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X71660; CAA50652.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S38190; S48379.

RefSeq

NP_012111.1; -.

3D structure databases

ModBase

P32191.

Organism-specific databases

YIL155c; -.

S000001417; GUT2.

Gene expression databases

ArrayExpress

P32191; -.

GermOnline

YIL155C; Saccharomyces cerevisiae.

Ontologies

GO:0009331;	Cellular component: glycerol-3-phosphate dehydrogenase complex (inferred from electronic annotation from InterPro).
GO:0031307;	Cellular component: integral to mitochondrial outer membrane (inferred from direct assay from SGD).
GO:0005743;	Cellular component: mitochondrial inner membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004368;	Molecular function: glycerol-3-phosphate dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0005975;	Biological process: carbohydrate metabolic process (inferred from mutant phenotype from SGD).
GO:0006072;	Biological process: glycerol-3-phosphate metabolic process (inferred from electronic annotation from InterPro).
GO:0006116;	Biological process: NADH oxidation (inferred from direct assay from SGD).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0001302;	Biological process: replicative cell aging (inferred from mutant phenotype from SGD).
QuickGo view.

Family and domain databases

InterPro

IPR000447; FAD-dep_Gly3P_DHase.
IPR006076; FAD-dep_OxRdtase.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

PRINTS

PR01001; FADG3PDH.

PROSITE

PS00977; FAD_G3PDH_1; 1.
PS00978; FAD_G3PDH_2; 1.

Proteomic databases

P32191; -.

Genome annotation databases

Ensembl

YIL155C; Saccharomyces cerevisiae. [Contig view]

854651; -.

Z47047_GR; YIL155C.

sce:YIL155C; -.

fig|4932.3.peg.1633; -.

Phylogenomic databases

HOGENOM

P32191; -.

Other

LinkHub

P32191; -.

NextBio

977202; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 649`		`Glycerol-3-phosphate dehydrogenase, mitochondrial.`	`PRO_0000010434`
`NP_BIND`	`69 97`	`29`	`FAD (Potential).`
`CONFLICT`	`1 51`		`MFSVTRRRAAGAAAAMATATGTLYWMTSQGDRPLVHNDPS` `YMVQFPTAAPP -> MTRATWCNSPPPLHR (in Ref. 1).`
`CONFLICT`	`63 63`		`A -> D (in Ref. 1; CAA50652).`
`CONFLICT`	`182 182`		`A -> G (in Ref. 1; CAA50652).`
`CONFLICT`	`234 234`		`A -> G (in Ref. 1; CAA50652).`
`CONFLICT`	`243 243`		`N -> I (in Ref. 1; CAA50652).`
`CONFLICT`	`320 320`		`A -> S (in Ref. 1; CAA50652).`
`CONFLICT`	`342 342`		`C -> S (in Ref. 1; CAA50652).`
`CONFLICT`	`645 646`		`KT -> QGR (in Ref. 1; CAA50652).`

Sequence information

Length: 649 AA [This is the length of the unprocessed precursor]

Molecular weight: 72389 Da [This is the MW of the unprocessed precursor]

CRC64: FE6B25F5B21EF8DA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP PQVSRRDLLD 

        70         80         90        100        110        120 
RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD FASGTSSKST KMIHGGVRYL 

       130        140        150        160        170        180 
EKAFWEFSKA QLDLVIEALN ERKHLINTAP HLCTVLPILI PIYSTWQVPY IYMGCKFYDF 

       190        200        210        220        230        240 
FAGSQNLKKS YLLSKSATVE KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT 

       250        260        270        280        290        300 
VLNYVEVQKL IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG 

       310        320        330        340        350        360 
LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD VRTSDGRVMF 

       370        380        390        400        410        420 
FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH YIEFPVKRED VLSAWAGVRP 

       430        440        450        460        470        480 
LVRDPRTIPA DGKKGSATQG VVRSHFLFTS DNGLITIAGG KWTTYRQMAE ETVDKVVEVG 

       490        500        510        520        530        540 
GFHNLKPCHT RDIKLAGAEE WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE 

       550        560        570        580        590        600 
SMENKLPLSL ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR 

       610        620        630        640 
TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV

P32191 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools