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UniProtKB/Swiss-Prot entry P32176

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FDOG_ECOLI
Primary accession number P32176
Secondary accession numbers P78131 Q2M8J1
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on February 26, 2008 (Sequence version 5)
Annotations were last modified on    November 25, 2008 (Entry version 86)
Name and origin of the protein
Protein name Formate dehydrogenase-O, major subunit [Precursor]
Synonyms EC 1.2.1.2
Formate dehydrogenase-O subunit alpha
FDH-Z subunit alpha
Aerobic formate dehydrogenase major subunit
Gene name
Name: fdoG
OrderedLocusNames: b3894, JW3865
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/21.15.3391; PubMed=8346018 [NCBI, ExPASy, EBI, Israel, Japan]
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.";
Nucleic Acids Res. 21:3391-3398(1993).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 252-261; 344-348 AND 822.
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-190, AND CHARACTERIZATION.
STRAIN=K12;
PubMed=8522521 [NCBI, ExPASy, EBI, Israel, Japan]
Abaibou H., Pommier J., Giordano G., Mandrand-Berthelot M.-A.;
"Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase.";
J. Bacteriol. 177:7141-7149(1995).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L19201; AAB03027.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAD13456.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77415.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X87583; CAA60887.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A65195; S40838.
RefSeq AP_003914.1; -.
NP_418330.1; -.
3D structure databases
HSSP P24183; 1KQF. [HSSP ENTRY / PDB]
SMR P32176; 34-1015.
ModBase P32176.
Protein-protein interaction databases
IntAct P32176; -.
Enzyme and pathway databases
BioCyc EcoCyc:FDOG-MON; -.
MetaCyc:FDOG-MON; -.
Organism-specific databases
EchoBASE EB1804; -.
EcoGene EG11858; fdoG.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from InterPro).
GO:0042597; Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0008863; Molecular function: formate dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030151; Molecular function: molybdenum ion binding (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0008430; Molecular function: selenium binding (inferred from electronic annotation from UniProtKB-KW).
GO:0045333; Biological process: cellular respiration (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR009010; Asp_de-COase-like_fold.
IPR006443; Formate_DH_asu_anaerob.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR006311; Tat.
Graphical view of domain structure.
Gene3D G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.
Pfam PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01553; formate-DH-alph; 1.
TIGR01409; TAT_signal_seq; 1.
PROSITE PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; 1.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P32176.
ProtoNet P32176.
Genome annotation databases
GeneID 948394; -.
GenomeReviews U00096_GR; b3894.
AP009048_GR; JW3865.
KEGG ecj:JW3865; -.
eco:b3894; -.
Phylogenomic databases
HOGENOM P32176; -.
Genome annotation databases
CMR P32176; b3894.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; Molybdenum; NAD; Oxidoreductase; Periplasm; Selenium; Selenocysteine; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1     33  33     Tat-type signal (Potential). 
CHAIN   34   1016  983     Formate dehydrogenase-O, major subunit. PRO_0000063223
METAL   50     50        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   53     53        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   57     57        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   92     92        Iron-sulfur (4Fe-4S) (By similarity). 
NON_STD   196    196        Selenocysteine. 
CONFLICT   252    261        GAKLIVIDPR -> RREADCDRSC (in Ref. 1; AAB03027). 
CONFLICT   344    348        ENGFA -> GKRLR (in Ref. 1; AAB03027). 
Sequence information
Length: 1016 AA [This is the length of the unprocessed precursor] Molecular weight: 112549 Da [This is the MW of the unprocessed precursor] CRC64: DED0952028055769 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MQVSRRQFFK ICAGGMAGTT AAALGFAPSV ALAETRQYKL LRTRETRNTC TYCSVGCGLL 

        70         80         90        100        110        120 
MYSLGDGAKN AKASIFHIEG DPDHPVNRGA LCPKGAGLVD FIHSESRLKF PEYRAPGSDK 

       130        140        150        160        170        180 
WQQISWEEAF DRIAKLMKED RDANYIAQNA EGVTVNRWLS TGMLCASASS NETGYLTQKF 

       190        200        210        220        230        240 
SRALGMLAVD NQARVUHGPT VASLAPTFGR GAMTNHWVDI KNANLVVVMG GNAAEAHPVG 

       250        260        270        280        290        300 
FRWAMEAKIH NGAKLIVIDP RFTRTAAVAD YYAPIRSGTD IAFLSGVLLY LLNNEKFNRE 

       310        320        330        340        350        360 
YTEAYTNASL IVREDYGFED GLFTGYDAEK RKYDKSSWTY ELDENGFAKR DTTLQHPRCV 

       370        380        390        400        410        420 
WNLLKQHVSR YTPDVVENIC GTPKDAFLKV CEYIAETSAH DKTASFLYAL GWTQHSVGAQ 

       430        440        450        460        470        480 
NIRTMAMIQL LLGNMGMAGG GVNALRGHSN IQGLTDLGLL SQSLPGYMTL PSEKQTDLQT 

       490        500        510        520        530        540 
YLTANTPKPL LEGQVNYWGN YPKFFVSMMK AFFGDKATAE NSWGFDWLPK WDKGYDVLQY 

       550        560        570        580        590        600 
FEMMKEGKVN GYICQGFNPV ASFPNKNKVI GCLSKLKFLV TIDPLNTETS NFWQNHGELN 

       610        620        630        640        650        660 
EVDSSKIQTE VFRLPSTCFA EENGSIVNSG RWLQWHWKGA DAPGIALTDG EILSGIFLRL 

       670        680        690        700        710        720 
RKMYAEQGGA NPDQVLNMTW NYAIPHEPSS EEVAMESNGK ALADITDPAT GAVIVKKGQQ 

       730        740        750        760        770        780 
LSSFAQLRDD GTTSCGCWIF AGSWTPEGNQ MARRDNADPS GLGNTLGWAW AWPLNRRILY 

       790        800        810        820        830        840 
NRASADPQGN PWDPKRQLLK WDGTKWTGWD IPDYSAAPPG SGVGPFIMQQ EGMGRLFALD 

       850        860        870        880        890        900 
KMAEGPFPEH YEPFETPLGT NPLHPNVISN PAARIFKDDA EALGKADKFP YVGTTYRLTE 

       910        920        930        940        950        960 
HFHYWTKHAL LNAILQPEQF VEIGESLANK LGIAQGDTVK VSSNRGYIKA KAVVTKRIRT 

       970        980        990       1000       1010 
LKANGKDIDT IGIPIHWGYE GVAKKGFIAN TLTPFVGDAN TQTPEFKSFL VNVEKV
P32176 in FASTA format

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View entry in raw text format (no links)
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