Fructose-like phosphotransferase enzyme IIB component
     (EC 2.7.1.69)
     (PTS system fructose-like EIIB component)
Fructose-like permease IIC component
     (PTS system fructose-like EIIC component)

Gene name

	Name:	frvB
	Synonyms:	yiiJ
	OrderedLocusNames:	b3899, JW5562

From

Escherichia coli (strain K12)

[TaxID: 83333]

[HAMAP proteome]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1093/nar/21.15.3391; PubMed=8346018 [NCBI, ExPASy, EBI, Israel, Japan] Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.; "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."; Nucleic Acids Res. 21:3391-3398(1993).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan] Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.; "The complete genome sequence of Escherichia coli K-12."; Science 277:1453-1474(1997).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan] Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."; Mol. Syst. Biol. 2:E1-E5(2006).
[4]	DISCUSSION OF SEQUENCE. PubMed=8019415 [NCBI, ExPASy, EBI, Israel, Japan] Reizer J., Michotey V., Reizer A., Saier M.H. Jr.; "Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems."; Protein Sci. 3:440-450(1994).
[5]	SUBCELLULAR LOCATION. STRAIN=K12 / MG1655 / ATCC 47076; DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan] Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.; "Global topology analysis of the Escherichia coli inner membrane proteome."; Science 308:1321-1323(2005).

Comments

FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (By similarity).
CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
SIMILARITY: Contains 1 PTS EIIB type-2 domain.
SIMILARITY: Contains 1 PTS EIIC type-2 domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L19201; AAB03032.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76881.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77410.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

AP_003909.1; -.
NP_418335.4; -.

3D structure databases

ModBase

P32154.

Enzyme and pathway databases

BioCyc

EcoCyc:FRVB-MON; -.

Organism-specific databases

EchoBASE

EB1809; -.

EcoGene

EG11863; frvB.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016301;	Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0008982;	Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351;	Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401;	Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR013011; PTS_EIIB_2.
IPR003352; PTS_EIIC.
IPR013014; PTS_EIIC_2.
IPR003353; PTS_IIB_fruc.
IPR006327; PTS_IIC_fruc.
Graphical view of domain structure.

Pfam

PF02378; PTS_EIIC; 1.
PF02379; PTS_IIB_fruc; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00829; FRU; 1.
TIGR01427; PTS_IIC_fructo; 1.

PROSITE

PS51099; PTS_EIIB_TYPE_2; 1.
PS51104; PTS_EIIC_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

948390; -.

GenomeReviews

U00096_GR; b3899.
AP009048_GR; JW5562.

KEGG

ecj:JW5562; -.
eco:b3899; -.

Phylogenomic databases

HOGENOM

P32154; -.

Genome annotation databases

CMR

P32154; b3899.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphotransferase system; Sugar transport; Transferase; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 483`	`483`	`Fructose-like PTS system EIIBC component.`	`PRO_0000186499`
`TRANSMEM`	`132 152`	`21`	`Potential.`
`TRANSMEM`	`180 200`	`21`	`Potential.`
`TRANSMEM`	`204 224`	`21`	`Potential.`
`TRANSMEM`	`227 247`	`21`	`Potential.`
`TRANSMEM`	`264 284`	`21`	`Potential.`
`TRANSMEM`	`303 323`	`21`	`Potential.`
`TRANSMEM`	`344 364`	`21`	`Potential.`
`TRANSMEM`	`380 400`	`21`	`Potential.`
`TRANSMEM`	`402 422`	`21`	`Potential.`
`TRANSMEM`	`442 462`	`21`	`Potential.`
`DOMAIN`	`1 105`	`105`	`PTS EIIB type-2.`
`DOMAIN`	`128 475`	`348`	`PTS EIIC type-2.`
`ACT_SITE`	`13 13`		`Phosphocysteine intermediate; for EIIB activity (By similarity).`

Sequence information

Length: 483 AA [This is the length of the unprocessed precursor]

Molecular weight: 51120 Da [This is the MW of the unprocessed precursor]

CRC64: 9B982ECDCBA1E1A4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MESSLRIVAI TNCPAGIAHT YMVAEALEQK ARSLGHTIKV ETQGSSGVEN RLSSEEIAAA 

        70         80         90        100        110        120 
DYVILATGRG LSGDDRARFA GKKVYEIAIS QALKNIDQIF SELPTNSQLF AADSGVKLGK 

       130        140        150        160        170        180 
QEVQSGSVMS HLMAGVSAAL PFVIGGGILV ALANMLVQFG LPYTDMSKGA PSFTWVVESI 

       190        200        210        220        230        240 
GYLGFTFMIP IMGAYIASSI ADKPAFAPAF LVCYLANDKA LLGTQSGAGF LGAVVLGLAI 

       250        260        270        280        290        300 
GYFVFWFRKV RLGKALQPLL GSMLIPFVTL LVFGVLTYYV IGPVMSDLMG GLLHFLNTIP 

       310        320        330        340        350        360 
PSMKFAAAFL VGAMLAFDMG GPINKTAWFF CFSLLEKHIY DWYAIVGVVA LMPPVAAGLA 

       370        380        390        400        410        420 
TFIAPKLFTR QEKEAASSAI VVGATVATEP AIPYALAAPL PMITANTLAG GITGVLVIAF 

       430        440        450        460        470        480 
GIKRLAPGLG IFDPLIGLMS PVGSFYLVLA IGLALNISFI IVLKGLWLRR KAKAAQQELV 


HEH

P32154 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools