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UniProtKB/Swiss-Prot entry P31974

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MTA1_CELCE
Primary accession number P31974
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on July 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 48)
Name and origin of the protein
Protein name Modification methylase AluI
Synonyms M.AluI
EC 2.1.1.37
Cytosine-specific methyltransferase AluI
Gene name
Name: aluIM
From
Cellulosimicrobium cellulans (Arthrobacter luteus) [TaxID: 1710] 
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Micrococcineae; Promicromonosporaceae; Cellulosimicrobium.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 21606 / DSM 20424 / 73-14;
DOI=10.1093/nar/21.4.905; PubMed=8451189 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang B., Tao T., Wilson G.G., Blumenthal R.M.;
"The M.AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region.";
Nucleic Acids Res. 21:905-911(1993).
Comments
  • FUNCTION: This methylase recognizes the double-stranded sequence AGCT, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the AluI endonuclease.
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
  • SIMILARITY: Belongs to the C5-methyltransferase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z11841; CAA77866.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S35614; S35614.
3D structure databases
HSSP P05102; 6MHT. [HSSP ENTRY / PDB]
ModBase P31974.
Ontologies
GO
GO:0003886; Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0006306; Biological process: DNA methylation (inferred from electronic annotation from InterPro).
GO:0009307; Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001525; C5_DNA_meth.
Graphical view of domain structure.
PANTHER PTHR10629; C5_DNA_meth; 1.
Pfam PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.
PRINTS PR00105; C5METTRFRASE.
TIGRFAMs TIGR00675; dcm; 1.
PROSITE PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; FALSE_NEG.
BLOCKS P31974.
ProtoNet P31974.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   521  521     Modification methylase AluI. PRO_0000087858
ACT_SITE   84    84        By similarity. 
Sequence information
Length: 521 AA [This is the length of the unprocessed precursor] Molecular weight: 58648 Da [This is the MW of the unprocessed precursor] CRC64: ACBBB2C4E5326BFB [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSKANAKYSF VDLFAGIGGF HAALAATGGV CEYAVEIDRE AAAVYERNWN KPALGDITDD 

        70         80         90        100        110        120 
ANDEGVTLRG YDGPIDVLTG GFPCQPFSKS GAQHGMAETR GTLFWNIARI IEEREPTVLI 

       130        140        150        160        170        180 
LENVRNLVGP RHRHEWLTII ETLRFFGYEV SGAPAIFSPH LLPAWMGGTP QVRERVFITA 

       190        200        210        220        230        240 
TLVPERMRDE RIPRTETGEI DAEAIGPKPV ATMNDRFPIK KGGTELFHPG DRKSGWNLLT 

       250        260        270        280        290        300 
SGIIREGDPE PSNVDLRLTE TETLWIDAWD DLESTIRRAT GRPLEGFPYW ADSWTDFREL 

       310        320        330        340        350        360 
SRLVVIRGFQ APEREVVGDR KRYVARTDMP EGFVPASVTR PAIDETLPAW KQSHLRRNYD 

       370        380        390        400        410        420 
FFERHFAEVV AWAYRWGVYT DLFPASRRKL EWQAQDAPRL WDTVMHFRPS GIRAKRPTYL 

       430        440        450        460        470        480 
PALVAITQTS IVGPLERRLS PRETARLQGL PEWFDFGEQR AAATYKQMGN GVNVGVVRHI 

       490        500        510        520 
LREHVRRDRA LLKLTPAGQR IINAVLADEP DATVGALGAA E
P31974 in FASTA format

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