[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-11. PubMed=1587288 [NCBI, ExPASy, EBI, Israel, Japan] Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A.; "The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes."; Eur. J. Biochem. 206:93-102(1992).
[2]	ERRATUM. PubMed=8319698 [NCBI, ExPASy, EBI, Israel, Japan] Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A.; Eur. J. Biochem. 214:949-950(1993).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=DSMZ 1740; DOI=10.1073/pnas.1932838100; PubMed=14500908 [NCBI, ExPASy, EBI, Israel, Japan] Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C.; "Complete genome sequence and analysis of Wolinella succinogenes."; Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + menaquinone = menaquinol.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X65189; CAA46303.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BX571661; CAE10713.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S33853; S33853.

RefSeq

NP_907813.1; -.

3D structure databases

HSSP

P18188; 1FRF. [HSSP ENTRY / PDB]

ModBase

P31883.

Enzyme and pathway databases

BioCyc

WSUC273121:WS1686-MON; -.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008901;	Molecular function: ferredoxin hydrogenase activity (inferred from electronic annotation from InterPro).
GO:0047067;	Molecular function: hydrogen:quinone oxidoreductase activity (inferred from electronic annotation from EC).
GO:0016151;	Molecular function: nickel ion binding (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Genome annotation databases

2554759; -.

BX571656_GR; WS1686.

wsu:WS1686; -.

fig|273121.1.peg.1540; -.

Phylogenomic databases

HOGENOM

P31883; -.

Genome annotation databases

CMR

P31883; WS1686.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 576`	`575`	`Quinone-reactive Ni/Fe-hydrogenase large chain.`	`PRO_0000199718`
`METAL`	`62 62`		`Nickel (Potential).`
`METAL`	`65 65`		`Nickel (Potential).`
`METAL`	`547 547`		`Nickel (Potential).`
`METAL`	`550 550`		`Nickel (Potential).`

Sequence information

Length: 576 AA [This is the length of the unprocessed precursor]

Molecular weight: 63977 Da [This is the MW of the unprocessed precursor]

CRC64: 091357213B6FF84E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTKRIVVDPI TRIEGHLRIE VVVDENNVIQ DAFSTATLWR GLETILKGRD PRDAGFFTQR 

        70         80         90        100        110        120 
ICGVCTYSHY KAGISAVENA LGIKPPLNAE LIRSLMSISL ILHDHTVHFY HLHGLDWCDI 

       130        140        150        160        170        180 
TSALKADPVA ASKLAFKYSP NPIATGADEL TAVQKRVAEF AAKGNLGPFA NAYWGHKTYR 

       190        200        210        220        230        240 
FSPEQNLIVL SHYLKALEVQ RVAAQMMAIW GAKQPHPQSL TVGGVTSVMD ALDPSRLGDW 

       250        260        270        280        290        300 
LTKYKYVADF VNRAYYADVV MAAEVFKSEP SVLGGCNVKN FYSYQEIPLN KTEWMYSTGI 

       310        320        330        340        350        360 
VMDGDITKVH EINEDLITEE ATHAWYKENK ALHPYDGQQD PNYTGFKDME TVGPDGTMVK 

       370        380        390        400        410        420 
TKVIDEKGKY TWIKAPRYGG KPLEVGPLAT IVVGLAAKNP RIEKVATQFL KDTGLPLAAL 

       430        440        450        460        470        480 
FTTLGRTAAR MLECKLSADY GFEAFNSLIA NLKVDQSTYT TYKIDKNKEY KGRYMGTVPR 

       490        500        510        520        530        540 
GVLSHWVRIK NGVIQNYQAV VPSTWNAGPR DANGTKGPYE ASLVGMKLQD LSQPLEIIRV 

       550        560        570 
IHSFDPCIAC AVHVMDTKGN ELSQYRVDPI TVGCNL

P31883 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools