ID   4CL1_SOYBN              Reviewed;         293 AA.
AC   P31686;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   04-NOV-2008, entry version 42.
DE   RecName: Full=4-coumarate--CoA ligase 1;
DE            Short=4CL 1;
DE            EC=6.2.1.12;
DE   AltName: Full=4-coumaroyl-CoA synthase 1;
DE   AltName: Full=Clone 4CL14;
DE   Flags: Fragment;
OS   Glycine max (Soybean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
OC   Glycine.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Harosoy 63;
RX   MEDLINE=94105342; PubMed=8278545; DOI=10.1104/pp.102.4.1147;
RA   Uhlmann A., Ebel J.;
RT   "Molecular cloning and expression of 4-coumarate:coenzyme A ligase, an
RT   enzyme involved in the resistance response of soybean (Glycine max L.)
RT   against pathogen attack.";
RL   Plant Physiol. 102:1147-1156(1993).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-coumarate + CoA = AMP + diphosphate +
CC       4-coumaroyl-CoA.
CC   -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC       biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step
CC       1/2.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme
CC       family.
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DR   EMBL; X69954; CAA49575.1; -; mRNA.
DR   PIR; S31705; S31705.
DR   HSSP; P08659; 1LCI.
DR   GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:EC.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   Pfam; PF00501; AMP-binding; 1.
DR   PROSITE; PS00455; AMP_BINDING; PARTIAL.
PE   2: Evidence at transcript level;
KW   Ligase; Phenylpropanoid metabolism.
FT   CHAIN        <1    293       4-coumarate--CoA ligase 1.
FT                                /FTId=PRO_0000193038.
FT   NON_TER       1      1
SQ   SEQUENCE   293 AA;  32027 MW;  25868497FCC022EC CRC64;
     AKATILLMPK FDINSLLALI HKHKVTIAPV VPPIVLAISK SPDLHKYDLS SIRVLKSGGA
     PLGKELEDTL RAKFPNAKLG QGYGMTEAGP VLTMSLAFAK EPIDVKPGAC GTVVRNAEMK
     IVDPETGHSL PRNQSGEICI RGDQIMKGYL NDGEATERTI DKDGWLHTGD IGYIDDDDEL
     FIVDRLKELI KYKGFQVAPA ELEALLLTHP KISDAAVVPM KDEAAGEVPV AFVVISNGYT
     DTTEDEIKQF ISKQVVFYKR INRVFFIDAI PKSPSGKILR KDLRAKIAAS VPK
//