NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Harosoy 63;
DOI=10.1104/pp.102.4.1147; PubMed=8278545 [NCBI, ExPASy, EBI, Israel, Japan]
Uhlmann A., Ebel J.;
"Molecular cloning and expression of 4-coumarate:coenzyme A ligase, an enzyme involved in the resistance response of soybean (Glycine max L.) against pathogen attack.";
Plant Physiol. 102:1147-1156(1993).

Comments

CATALYTIC ACTIVITY: ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2 .
SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X69954; CAA49575.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S31705; S31705.

3D structure databases

HSSP

P08659; 1LCI. [HSSP ENTRY / PDB]

ModBase

P31686.

Ontologies

GO:0016207;	Molecular function: 4-coumarate-CoA ligase activity (inferred from electronic annotation from EC).
GO:0009698;	Biological process: phenylpropanoid metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.

Pfam

PF00501; AMP-binding; 1.
Pfam graphical view of domain structure.

PROSITE

PS00455; AMP_BINDING; PARTIAL.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Ligase; Phenylpropanoid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 293`	`>293`	`4-coumarate--CoA ligase 1.`	`PRO_0000193038`
`NON_TER`	`1 1`

Sequence information

Length: 293 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 32027 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 25868497FCC022EC [This is a checksum on the sequence]

        10         20         30         40         50         60 
AKATILLMPK FDINSLLALI HKHKVTIAPV VPPIVLAISK SPDLHKYDLS SIRVLKSGGA 

        70         80         90        100        110        120 
PLGKELEDTL RAKFPNAKLG QGYGMTEAGP VLTMSLAFAK EPIDVKPGAC GTVVRNAEMK 

       130        140        150        160        170        180 
IVDPETGHSL PRNQSGEICI RGDQIMKGYL NDGEATERTI DKDGWLHTGD IGYIDDDDEL 

       190        200        210        220        230        240 
FIVDRLKELI KYKGFQVAPA ELEALLLTHP KISDAAVVPM KDEAAGEVPV AFVVISNGYT 

       250        260        270        280        290 
DTTEDEIKQF ISKQVVFYKR INRVFFIDAI PKSPSGKILR KDLRAKIAAS VPK

P31686 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools