ID   GBRA5_HUMAN             Reviewed;         462 AA.
AC   P31644; Q14DC2; Q53XL6; Q9NYT3; Q9NYT4; Q9NYT5;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 91.
DE   RecName: Full=Gamma-aminobutyric acid receptor subunit alpha-5;
DE   AltName: Full=GABA(A) receptor subunit alpha-5;
DE   Flags: Precursor;
GN   Name=GABRA5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=93271965; PubMed=8388764; DOI=10.1093/hmg/2.2.183;
RA   Knoll J.H., Sinnett D., Wagstaff J., Glatt K.A., Wilcox A.S.,
RA   Whiting P.J., Wingrove P., Sikela J.M., Lalande M.;
RT   "FISH ordering of reference markers and of the gene for the alpha 5
RT   subunit of the gamma-aminobutyric acid receptor (GABRA5) within the
RT   Angelman and Prader-Willi syndrome chromosomal regions.";
RL   Hum. Mol. Genet. 2:183-189(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=92339652; PubMed=1321750;
RA   Wingrove P., Hadingham K., Wafford K., Kemp J.A., Ragan C.I.,
RA   Whiting P.;
RT   "Cloning and expression of a cDNA encoding the human GABA-A receptor
RT   alpha 5 subunit.";
RL   Biochem. Soc. Trans. 20:18S-18S(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-166; 195-241 AND 294-363.
RA   Kim S.-J., Gonen D., Yang Z.-Y., Reliford A., Dy A., Leventhal B.L.,
RA   Cook E.H. Jr.;
RT   "Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 5
RT   subunit gene (GABRA5).";
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
RC   TISSUE=Fetal brain;
RX   MEDLINE=97349104; PubMed=9205108; DOI=10.1006/geno.1997.4770;
RA   Kim Y., Glatt H., Xie W., Sinnett D., Lalande M.;
RT   "Human gamma-aminobutyric acid-type A receptor alpha5 subunit gene
RT   (GABRA5): characterization and structural organization of the 5'
RT   flanking region.";
RL   Genomics 42:378-387(1997).
RN   [7]
RP   UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-355, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17370265; DOI=10.1002/pmic.200600410;
RA   Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.;
RT   "Tryptic digestion of ubiquitin standards reveals an improved strategy
RT   for identifying ubiquitinated proteins by mass spectrometry.";
RL   Proteomics 7:868-874(2007).
CC   -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the
CC       vertebrate brain, mediates neuronal inhibition by binding to the
CC       GABA/benzodiazepine receptor and opening an integral chloride
CC       channel.
CC   -!- SUBUNIT: Generally pentameric. There are five types of GABA(A)
CC       receptor chains: alpha, beta, gamma, delta, and rho.
CC   -!- SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell
CC       membrane; Multi-pass membrane protein. Cell membrane; Multi-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9)
CC       family.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=Forbidden fruit - Issue
CC       56 of March 2005;
CC       URL="http://www.expasy.org/spotlight/back_issues/sptlt056.shtml";
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DR   EMBL; L08485; AAA58490.1; -; mRNA.
DR   EMBL; BT009830; AAP88832.1; -; mRNA.
DR   EMBL; BC111979; AAI11980.1; -; mRNA.
DR   EMBL; BC113422; AAI13423.1; -; mRNA.
DR   EMBL; AF228447; AAF62508.1; -; Genomic_DNA.
DR   EMBL; AF228448; AAF62509.1; -; Genomic_DNA.
DR   EMBL; AF228449; AAF62510.1; -; Genomic_DNA.
DR   EMBL; AF061785; AAC31809.1; -; Genomic_DNA.
DR   PIR; A48933; A48933.
DR   RefSeq; NP_000801.1; -.
DR   UniGene; Hs.612087; -.
DR   Ensembl; ENSG00000186297; Homo sapiens.
DR   GeneID; 2558; -.
DR   KEGG; hsa:2558; -.
DR   H-InvDB; HIX0026792; -.
DR   HGNC; HGNC:4079; GABRA5.
DR   MIM; 137142; gene.
DR   PharmGKB; PA28493; -.
DR   HOGENOM; P31644; -.
DR   HOVERGEN; P31644; -.
DR   DrugBank; DB00404; Alprazolam.
DR   DrugBank; DB00189; Ethchlorvynol.
DR   DrugBank; DB01544; Flunitrazepam.
DR   DrugBank; DB00690; Flurazepam.
DR   DrugBank; DB00186; Lorazepam.
DR   DrugBank; DB00371; Meprobamate.
DR   DrugBank; DB00683; Midazolam.
DR   NextBio; 10103; -.
DR   ArrayExpress; P31644; -.
DR   CleanEx; HS_GABRA5; -.
DR   GermOnline; ENSG00000186297; Homo sapiens.
DR   GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR   GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR   GO; GO:0045211; C:postsynaptic membrane; IEA:InterPro.
DR   GO; GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
DR   GO; GO:0031404; F:chloride ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0005230; F:extracellular ligand-gated ion channel acti...; IEA:InterPro.
DR   GO; GO:0004890; F:GABA-A receptor activity; IEA:InterPro.
DR   GO; GO:0030594; F:neurotransmitter receptor activity; IEA:InterPro.
DR   GO; GO:0006821; P:chloride transport; IEA:InterPro.
DR   GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IEA:InterPro.
DR   InterPro; IPR006028; GABAA_rcpt.
DR   InterPro; IPR001390; GABAAa_rcpt.
DR   InterPro; IPR005435; GABBAa5_rcpt.
DR   InterPro; IPR006029; Neu_channel_TM.
DR   InterPro; IPR006202; Neur_chan_lig_bd.
DR   InterPro; IPR006201; Neur_channel.
DR   Gene3D; G3DSA:2.70.170.10; Neur_chan_lig_bd; 1.
DR   PANTHER; PTHR18945; Neur_channel; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   Pfam; PF02932; Neur_chan_memb; 1.
DR   PRINTS; PR01079; GABAARALPHA.
DR   PRINTS; PR01618; GABAARALPHA5.
DR   PRINTS; PR00253; GABAARECEPTR.
DR   PRINTS; PR00252; NRIONCHANNEL.
DR   TIGRFAMs; TIGR00860; LIC; 1.
DR   PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE   1: Evidence at protein level;
KW   Cell junction; Cell membrane; Chloride; Chloride channel;
KW   Glycoprotein; Ion transport; Ionic channel; Membrane;
KW   Postsynaptic cell membrane; Signal; Synapse; Transmembrane; Transport;
KW   Ubl conjugation.
FT   SIGNAL        1     31       Potential.
FT   CHAIN        32    462       Gamma-aminobutyric acid receptor subunit
FT                                alpha-5.
FT                                /FTId=PRO_0000000444.
FT   TOPO_DOM     32    259       Extracellular (Potential).
FT   TRANSMEM    260    281       Potential.
FT   TRANSMEM    286    307       Potential.
FT   TRANSMEM    319    341       Potential.
FT   TOPO_DOM    342    427       Cytoplasmic (Potential).
FT   TRANSMEM    428    449       Potential.
FT   CARBOHYD     45     45       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    145    145       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    207    207       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    236    236       N-linked (GlcNAc...) (Potential).
FT   DISULFID    173    187       By similarity.
FT   CROSSLNK    355    355       Glycyl lysine isopeptide (Lys-Gly)
FT                                (interchain with G-Cter in ubiquitin).
SQ   SEQUENCE   462 AA;  52146 MW;  260A8B554113AFF9 CRC64;
     MDNGMFSGFI MIKNLLLFCI SMNLSSHFGF SQMPTSSVKD ETNDNITIFT RILDGLLDGY
     DNRLRPGLGE RITQVRTDIY VTSFGPVSDT EMEYTIDVFF RQSWKDERLR FKGPMQRLPL
     NNLLASKIWT PDTFFHNGKK SIAHNMTTPN KLLRLEDDGT LLYTMRLTIS AECPMQLEDF
     PMDAHACPLK FGSYAYPNSE VVYVWTNGST KSVVVAEDGS RLNQYHLMGQ TVGTENISTS
     TGEYTIMTAH FHLKRKIGYF VIQTYLPCIM TVILSQVSFW LNRESVPART VFGVTTVLTM
     TTLSISARNS LPKVAYATAM DWFIAVCYAF VFSALIEFAT VNYFTKRGWA WDGKKALEAA
     KIKKKREVIL NKSTNAFTTG KMSHPPNIPK EQTPAGTSNT TSVSVKPSEE KTSESKKTYN
     SISKIDKMSR IVFPVLFGTF NLVYWATYLN REPVIKGAAS PK
//