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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Embryo;
DOI=10.1016/0012-1606(92)90110-3; PubMed=1397682 [NCBI, ExPASy, EBI, Israel, Japan]
Yasumasu S.,
Yamada K.,
Akasaka K.,
Mitsunaga K.,
Iuchi I.,
Shimada H.,
Yamagami K.;
"Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development.";
Dev. Biol. 153:250-258(1992).
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- FUNCTION: Participates in the breakdown of the egg envelope, which is derived from the egg extracellular matrix, at the time of hatching. Thus allowing the newly hatched fish to swim free. HCE binds tightly to the egg envelope while it exerts the choriolytic swelling action.
- CATALYTIC ACTIVITY: Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-|-7-(4-methyl)coumarylamide.
- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
- SUBCELLULAR LOCATION: Note=Stored as proenzymes in the zymogen granules.
- DEVELOPMENTAL STAGE: Production of the protein starts in day 2 to day 3 embryos and increases thereafter until hatching.
- PTM: O-glycosylated (Probable).
- MISCELLANEOUS: In medaka the hatching enzyme system is composed of two distinct proteases, the high choriolytic enzyme (HCE), of which there are two isoforms, and the low choriolytic enzyme (LCE).
- SIMILARITY: Belongs to the peptidase M12A family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 279 AA [This is the length of the unprocessed precursor] |
Molecular weight: 31490 Da [This is the MW of the unprocessed precursor] |
CRC64: 00244107A8B01B8C [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNLASSACLL LLFLLGIAQA LPVQNEEGHE EGNKEGHGEE GVEEGDEDDF VDFTTRILTS
70 80 90 100 110 120
NNNTDQLLLE GDLVAPTNRN AMKCWYNSCF WKKASNGFVV IPYVISSQYS RGEVATIEGA
130 140 150 160 170 180
MRAFNGRTCI RFVRRTNEYD FISVVSKNGC YSELGRKGGQ QELSLNRGGC MYSGIIQHEL
190 200 210 220 230 240
NHALGFQHEQ TRSDRDSYVR INWQNIIPAS AYNFNKHDTN NLNTPYDYSS IMHYGRDAFS
250 260 270
IAYGRDSITP IPNPNVPIGQ RNGMSRWDIT RSNVLYNCR
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P31581 in FASTA format |
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ExPASy Home page
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