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[1]
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NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 71-119 AND 208-223.
TISSUE=Embryo;
DOI=10.1016/0012-1606(92)90110-3; PubMed=1397682 [NCBI, ExPASy, EBI, Israel, Japan]
Yasumasu S.,
Yamada K.,
Akasaka K.,
Mitsunaga K.,
Iuchi I.,
Shimada H.,
Yamagami K.;
"Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development.";
Dev. Biol. 153:250-258(1992).
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- FUNCTION: Participates in the breakdown of the egg envelope, which is derived from the egg extracellular matrix, at the time of hatching. Thus allowing the newly hatched fish to swim free. HCE binds tightly to the egg envelope while it exerts the choriolytic swelling action.
- CATALYTIC ACTIVITY: Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-|-7-(4-methyl)coumarylamide.
- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
- SUBCELLULAR LOCATION: Note=Stored as proenzymes in the zymogen granules.
- DEVELOPMENTAL STAGE: Production of the protein starts in day 2 to day 3 embryos and increases thereafter until hatching.
- PTM: O-glycosylated (Probable).
- MISCELLANEOUS: In medaka the hatching enzyme system is composed of two distinct proteases, the high choriolytic enzyme (HCE), of which there are two isoforms, and the low choriolytic enzyme (LCE).
- SIMILARITY: Belongs to the peptidase M12A family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 270 AA [This is the length of the unprocessed precursor] |
Molecular weight: 30392 Da [This is the MW of the unprocessed precursor] |
CRC64: D85C972906E3735A [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNLAPSTCLL LLFLLDIAQA LPVWDEEGHE EGHEEGDGDD FVDITTRILT SNNNTDQLLL
70 80 90 100 110 120
EGDLVAPTNR NAMKCWSSSC FWKKASNGLV VIPYVISSEY SGGEVATIEG AMRAFNGKTC
130 140 150 160 170 180
IRFVRRTNEY DFISVVSKTG CYSELGRKGG QQELSINRGG CMYSGIIQHE LNHALGFQHE
190 200 210 220 230 240
QTRSDRDSYV RINWENIIPA SAYNFNKHDT NNLNTPYDYS SIMHYGRDAF SIAYGRDSIT
250 260 270
PIPNPNVPIG QRNGMSRWDI TRINVLYNCR
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P31580 in FASTA format |
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ExPASy Home page
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