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[1]
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NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 72-96.
TISSUE=Embryo;
DOI=10.1016/0012-1606(92)90110-3; PubMed=1397682 [NCBI, ExPASy, EBI, Israel, Japan]
Yasumasu S.,
Yamada K.,
Akasaka K.,
Mitsunaga K.,
Iuchi I.,
Shimada H.,
Yamagami K.;
"Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development.";
Dev. Biol. 153:250-258(1992).
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[2]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DRR;
PubMed=8647122 [NCBI, ExPASy, EBI, Israel, Japan]
Yasumasu S.,
Shimada H.,
Inohaya K.,
Yamazaki K.,
Iuchi I.,
Yasumasu I.,
Yamagami K.;
"Different exon-intron organizations of the genes for two astacin-like proteases, high choriolytic enzyme (choriolysin H) and low choriolytic enzyme (choriolysin L), the constituents of the fish hatching enzyme.";
Eur. J. Biochem. 237:752-758(1996).
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- FUNCTION: Participates in the breakdown of the egg envelope, which is derived from the egg extracellular matrix, at the time of hatching. Thus allowing the newly hatched fish to swim free. LCE solubilizes the egg envelope only after it has been swollen by the action of HCE.
- CATALYTIC ACTIVITY: Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-|-7-(4-methyl)coumarylamide.
- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
- SUBCELLULAR LOCATION: Note=Stored as proenzymes in the zymogen granules.
- DEVELOPMENTAL STAGE: Production of the protein starts in day 2 to day 3 embryos and increases thereafter until hatching.
- PTM: O-glycosylated (Probable).
- MISCELLANEOUS: In medaka the hatching enzyme system is composed of two distinct proteases, the high choriolytic enzyme (HCE), of which there are two isoforms, and the low choriolytic enzyme (LCE).
- SIMILARITY: Belongs to the peptidase M12A family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 271 AA [This is the length of the unprocessed precursor] |
Molecular weight: 30985 Da [This is the MW of the unprocessed precursor] |
CRC64: 98B1342E2F7AA581 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MDLLAKASVL LLLLLSLSNA QTDNMEEAEN GSSKEEIDES ELEDVSSIIF RMNNNSMEEL
70 80 90 100 110 120
LEGDLVLPKT RNAMKCFGAP DSCRWPKSSN GIVKVPYVVS DNYESDEKET IRNAMKEFAE
130 140 150 160 170 180
KTCIHFVPRN NERAYLSLEP RFGCKSMMGY VGDKQVVVLQ RFGCIKHAVI QHELLHALGF
190 200 210 220 230 240
YHEHTRSDRD QHVKINWENI IKDFTHNFDK NDTDNLGTPY DYGSIMHYGR TAFGKDRKET
250 260 270
ITPIPNPKAA IGQTERMSDI DILRVNKLYK C
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P31579 in FASTA format |
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