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UniProtKB/Swiss-Prot entry P31539


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name HS104_YEAST
Primary accession number P31539
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 79)
Name and origin of the protein
Protein name Heat shock protein 104
Synonyms None
Gene name
Name: HSP104
OrderedLocusNames: YLL026W
ORFNames: L0948
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 26109 / X2180;
DOI=10.1038/353270a0; PubMed=1896074 [NCBI, ExPASy, EBI, Israel, Japan]
Parsell D.A., Sanchez Y., Stitzel J.D., Lindquist S.;
"Hsp104 is a highly conserved protein with two essential nucleotide-binding sites.";
Nature 353:270-273(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169871 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
Nature 387:87-90(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 749-908.
STRAIN=ATCC 204508 / S288c;
DOI=10.1002/(SICI)1097-0061(199702)13:2<183::AID-YEA65>3.3.CO;2-M; PubMed=9046100 [NCBI, ExPASy, EBI, Israel, Japan]
Purnelle B., Goffeau A.;
"The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein.";
Yeast 13:183-188(1997).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-620, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.2135500100; PubMed=14557538 [NCBI, ExPASy, EBI, Israel, Japan]
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.;
"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.";
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-87; SER-155; THR-162; SER-206; SER-306; THR-499; SER-535; SER-577; SER-578 AND SER-768, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M67479; AAA50477.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z73131; CAA97475.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z73130; CAA97474.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY693002; AAT93021.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X97560; CAA66164.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S61476; S61476.
RefSeq NP_013074.1; -.
3D structure databases
HSSP P03815; 1JBK. [HSSP ENTRY / PDB]
ModBase P31539.
Protein-protein interaction databases
DIP DIP:2252N; -.
IntAct P31539; -.
2D gel databases
SWISS-2DPAGE P31539; -.
Organism-specific databases
CYGD YLL026w; -.
SGD S000003949; HSP104.
Yeast-GFP YLL026W.
Gene expression databases
ArrayExpress P31539; -.
GermOnline YLL026W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005634; Cellular component: nucleus (inferred from direct assay from SGD).
GO:0042623; Molecular function: ATPase activity, coupled (inferred from direct assay from SGD).
GO:0051087; Molecular function: chaperone binding (inferred from direct assay from SGD).
GO:0051085; Biological process: chaperone cofactor-dependent protein folding (inferred from direct assay from SGD).
GO:0042026; Biological process: protein refolding (inferred from direct assay from SGD).
GO:0006950; Biological process: response to stress (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR003593; AAA+_ATPase_core.
IPR003959; AAA_ATPase_core.
IPR013093; ATPase_AAA-2.
IPR001270; Chaprnin_clpA/B.
IPR004176; Clp_N.
Graphical view of domain structure.
Pfam PF00004; AAA; 1.
PF07724; AAA_2; 1.
PF02861; Clp_N; 2.
Pfam graphical view of domain structure.
PRINTS PR00300; CLPPROTEASEA.
SMART SM00382; AAA; 2.
SMART graphical view of domain structure.
PROSITE PS00870; CLPAB_1; 1.
PS00871; CLPAB_2; 1.
BLOCKS P31539.
Proteomic databases
PeptideAtlas P31539; -.
Genome annotation databases
Ensembl YLL026W; Saccharomyces cerevisiae. [Contig view]
GeneID 850633; -.
GenomeReviews Y13138_GR; YLL026W.
KEGG sce:YLL026W; -.
NMPDR fig|4932.3.peg.4065; -.
Phylogenomic databases
HOGENOM P31539; -.
Other
LinkHub P31539; -.
ProtoNet P31539.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Nucleus; Phosphoprotein; Repeat; Stress response; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   908  908     Heat shock protein 104. PRO_0000191212
NP_BIND   212   219  8     ATP (Potential). 
NP_BIND   614   621  8     ATP (Potential). 
REGION   167   411  245     I. 
REGION   541   731  191     II. 
MOD_RES   87    87        Phosphothreonine. 
MOD_RES   155   155        Phosphoserine. 
MOD_RES   162   162        Phosphothreonine. 
MOD_RES   206   206        Phosphoserine. 
MOD_RES   306   306        Phosphoserine. 
MOD_RES   499   499        Phosphothreonine. 
MOD_RES   535   535        Phosphoserine. 
MOD_RES   577   577        Phosphoserine. 
MOD_RES   578   578        Phosphoserine. 
MOD_RES   768   768        Phosphoserine. 
CROSSLNK   620   620        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
Sequence information
Length: 908 AA [This is the length of the unprocessed precursor] Molecular weight: 102035 Da [This is the MW of the unprocessed precursor] CRC64: 4AD0E7E3AF98E318 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNDQTQFTER ALTILTLAQK LASDHQHPQL QPIHILAAFI ETPEDGSVPY LQNLIEKGRY 

        70         80         90        100        110        120 
DYDLFKKVVN RNLVRIPQQQ PAPAEITPSY ALGKVLQDAA KIQKQQKDSF IAQDHILFAL 

       130        140        150        160        170        180 
FNDSSIQQIF KEAQVDIEAI KQQALELRGN TRIDSRGADT NTPLEYLSKY AIDMTEQARQ 

       190        200        210        220        230        240 
GKLDPVIGRE EEIRSTIRVL ARRIKSNPCL IGEPGIGKTA IIEGVAQRII DDDVPTILQG 

       250        260        270        280        290        300 
AKLFSLDLAA LTAGAKYKGD FEERFKGVLK EIEESKTLIV LFIDEIHMLM GNGKDDAANI 

       310        320        330        340        350        360 
LKPALSRGQL KVIGATTNNE YRSIVEKDGA FERRFQKIEV AEPSVRQTVA ILRGLQPKYE 

       370        380        390        400        410        420 
IHHGVRILDS ALVTAAQLAK RYLPYRRLPD SALDLVDISC AGVAVARDSK PEELDSKERQ 

       430        440        450        460        470        480 
LQLIQVEIKA LERDEDADST TKDRLKLARQ KEASLQEELE PLRQRYNEEK HGHEELTQAK 

       490        500        510        520        530        540 
KKLDELENKA LDAERRYDTA TAADLRYFAI PDIKKQIEKL EDQVAEEERR AGANSMIQNV 

       550        560        570        580        590        600 
VDSDTISETA ARLTGIPVKK LSESENEKLI HMERDLSSEV VGQMDAIKAV SNAVRLSRSG 

       610        620        630        640        650        660 
LANPRQPASF LFLGLSGSGK TELAKKVAGF LFNDEDMMIR VDCSELSEKY AVSKLLGTTA 

       670        680        690        700        710        720 
GYVGYDEGGF LTNQLQYKPY SVLLFDEVEK AHPDVLTVML QMLDDGRITS GQGKTIDCSN 

       730        740        750        760        770        780 
CIVIMTSNLG AEFINSQQGS KIQESTKNLV MGAVRQHFRP EFLNRISSIV IFNKLSRKAI 

       790        800        810        820        830        840 
HKIVDIRLKE IEERFEQNDK HYKLNLTQEA KDFLAKYGYS DDMGARPLNR LIQNEILNKL 

       850        860        870        880        890        900 
ALRILKNEIK DKETVNVVLK KGKSRDENVP EEAEECLEVL PNHEATIGAD TLGDDDNEDS 


MEIDDDLD 

P31539 in FASTA format

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View entry in raw text format (no links)
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