RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))

[TaxID: 33727]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Filoviridae; Marburgvirus.

Virus hosts

Cercopithecus aethiops (Green monkey) (Grivet) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1016/0042-6822(92)90456-Y; PubMed=1546452 [NCBI, ExPASy, EBI, Israel, Japan] Muehlberger E., Sanchez A., Randolf A., Will C., Kiley M.P., Klenk H.-D., Feldmann H.; "The nucleotide sequence of the L gene of Marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses."; Virology 187:534-547(1992).
[2]	SEQUENCE REVISION. Feldmann H.; Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=pp3/guinea pig lethal, and pp4/guinea pig nonlethal; Chain P.S.G., Malfatti S.A., Hajjaj A., Vergez L.M., Do L.H., Smith K.L., McCready P.M.; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=pp3/guinea pig lethal, and pp4/guinea pig nonlethal; Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
[5]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate Enterlein; DOI=10.1128/JVI.80.2.1038-1043.2006; PubMed=16379005 [NCBI, ExPASy, EBI, Israel, Japan] Enterlein S., Volchkov V., Weik M., Kolesnikova L., Volchkova V., Klenk H.-D., Muehlberger E.; "Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30."; J. Virol. 80:1038-1043(2006).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBCELLULAR LOCATION: Cytoplasm. Virion (By similarity).
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M92834; AAA46562.1; ALT_SEQ; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z12132; CAA78120.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY430365; AAR85466.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY430366; AAR85459.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ217792; ABA87130.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A42450; RRIWMV.

3D structure databases

ModBase

P31352.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012;	Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968;	Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370;	Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410;	Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR017235; RNA-dir_RNA_pol_filovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037548; RNA_pol_Filoviridae; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2331`	`2331`	`Large structural protein.`	`PRO_0000222169`
`DOMAIN`	`628 812`	`185`	`RdRp catalytic.`
`VARIANT`	`489 489`	`1`	`L -> A (in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein).`
`VARIANT`	`741 741`	`1`	`S -> C (in strain: pp3/guinea pig lethal).`
`VARIANT`	`979 979`	`1`	`R -> G (in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein).`
`VARIANT`	`1428 1428`	`1`	`S -> T (in strain: Isolate Enterlein).`

Sequence information

Length: 2331 AA [This is the length of the unprocessed precursor]

Molecular weight: 267093 Da [This is the MW of the unprocessed precursor]

CRC64: 1D54C60DA1BED3CF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQHPTQYPDA RLSSPIILDQ CDLLARSLGL YSHYSHNPKL RNCRIPHHIY RLRNSTALKT 

        70         80         90        100        110        120 
FLQNCSILTV PFHSIWDHIL TSIQYDAINH VDDFKYLLPS ELVKYANWDN EFLKAYLNKI 

       130        140        150        160        170        180 
LGLDHVFSAS ARSQCEDFSP KENPYYWGML LLVHLSQLAR RIKGQRGSLR SNWKFIGTDL 

       190        200        210        220        230        240 
ELFGIADFVI FKVPVKTIIR NAVSLQASKP GLRIWYRDQN LTPYLCDDEF IVSVASYECF 

       250        260        270        280        290        300 
IMIKDVFIER YNTWEICARA WLEDSDGADY PPLDVLGELY NQGDQIIAMY LEDGFKLIKH 

       310        320        330        340        350        360 
LEPLCVSCIQ THGIFTPRKY WFQSQMIKSY YDELHDLNLK LQISDNKAEC AQNFIKTIVQ 

       370        380        390        400        410        420 
AKLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQSTK ILKPKVMFET FCVFKFIVAK 

       430        440        450        460        470        480 
NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS FPSQAEIYQH LWEWYFVEHE PLFSTKIISD 

       490        500        510        520        530        540 
LSIFIKDRLT AVNQECWDSV FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILEFAEK 

       550        560        570        580        590        600 
LEYLAPSYRN FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT 

       610        620        630        640        650        660 
EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH FIDYCNRCYG 

       670        680        690        700        710        720 
VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT EDNRNNPPDC ANAYHYHLGG IEGLQQKLWT 

       730        740        750        760        770        780 
CISCAQITLV ELKTKLKLKS SVMGDNQCIT TLSLFPIDAP NDYQENEAEL NAARVAVELA 

       790        800        810        820        830        840 
ITTGYSGIFL KPEETFVHSG FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL 

       850        860        870        880        890        900 
ASIGTSFERG TSETRHIFPS RWIASFHSML AINLLNQNHL GFPLGFNIDI SCFKKPLTFS 

       910        920        930        940        950        960 
EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLK NALEFLEKEE LFYILISKKP 

       970        980        990       1000       1010       1020 
GLADASDFVM NPLGLNVPRS KEIITFLRQT VRENITITSQ NRIINSLFHI GSDLEDQRVC 

      1030       1040       1050       1060       1070       1080 
EWLLSSNPVM SRFAADIFSR TPSGKRLQVL GYLEGTRTLL ASRTISLTTE GTMLMKLREL 

      1090       1100       1110       1120       1130       1140 
TRNRWKSWFS YIDALDDDLS ESLEKFTCTV DVANFLRAYS WSDVLKGKRL IGATLPCLLE 

      1150       1160       1170       1180       1190       1200 
QFEVKWINLS EDLREQFNLS SDSKSTINLL PYDCKELRLE GSNDTELNYV SCALDRKVVQ 

      1210       1220       1230       1240       1250       1260 
KHPSVNRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR LLWVTQGTAD 

      1270       1280       1290       1300       1310       1320 
REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY GQHSFMANRM SNTSTRAIIS 

      1330       1340       1350       1360       1370       1380 
TNTLGKYAGG GQAAIDSNII FQNTINLGVA VLDIALSLAK LSSASNVTFR LMLNKCCTRH 

      1390       1400       1410       1420       1430       1440 
VPSEYLYFDK PLDVDLNKYM DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD 

      1450       1460       1470       1480       1490       1500 
FPTIAAWTLG ETIVGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE 

      1510       1520       1530       1540       1550       1560 
SLSLSRIKSI KNLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH HIPLISLMLG 

      1570       1580       1590       1600       1610       1620 
GSAGEKSSSD AVRLFLTASY QNFINNFSCL MKKGQSSLPV WLYFPSEGQQ LKPILKILQR 

      1630       1640       1650       1660       1670       1680 
LSDLLSPDKI QKRKILADTC CPIGSFWVYP SKSTRTNHYY ASLNYWRDKA NKVKNTPFSH 

      1690       1700       1710       1720       1730       1740 
LINCSFPEFS SHTSSVSSNQ QVTNSKYIVY PENITEINAR TRLINYGSTA LQGMDTKMPL 

      1750       1760       1770       1780       1790       1800 
SEQNLVENCR PSEGIRFKDN QKITKHDQRC EREESSPQQM FPEDNMQTPA HIHSSSPFQI 

      1810       1820       1830       1840       1850       1860 
LIKSLDAHED FDASKIILNS EINNLNLTEY TLNTKLLTTP TRTEILDTSP LQSSRYSSTS 

      1870       1880       1890       1900       1910       1920 
RERSLLSREQ ASYLYVDCSN IPSISLDPGF RSMSDQNQVQ MLINTYKRDL HACFDSNQFC 

      1930       1940       1950       1960       1970       1980 
RFTGVVSSMH YKLYDLLPPG KLKKAICLAE GEGSGARLLL KWKETDYLFF NTLATDSQQE 

      1990       2000       2010       2020       2030       2040 
AEILSGRVIP RMLYNIDRLS ALLESRRLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL 

      2050       2060       2070       2080       2090       2100 
TMDAETTKDE TREQLYKTIV NIWTRTSPNI PKISIIKVFL LDYEGTLFLM KNAIQYYGQV 

      2110       2120       2130       2140       2150       2160 
QLKKPYSSNA KNSEWYLCCG KRRIQRLQID FSDQVGIFLI CKAMSRQRQA IPYWLKHIEK 

      2170       2180       2190       2200       2210       2220 
NYPASLHEFF LTLGFPSLES SFCHRYTIPF SEGKALFHKV QSYVRQGKQH LHSLMLDYEN 

      2230       2240       2250       2260       2270       2280 
NSPLLDLRNH FICSLRGKIT KYYNDILKLN LVIKAVEKGK NWSQLVEILP NMHSVCIVHV 

      2290       2300       2310       2320       2330 
DHECSGCEKR LLLKLDFIRN TKIAEQKLLN RVIGYILFFP FGLFKSGSLR A

P31352 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools