ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P31332

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name L_SYNV
Primary accession number P31332
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on July 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 53)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Sonchus yellow net virus (SYNV) [TaxID: 11307] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus.
Virus hosts Aphis [TaxID: 80764]
Bidens pilosa (Hairy beggarticks) (Cobbler's pegs) [TaxID: 42337]
Lactuca sativa (Garden lettuce) [TaxID: 4236]
Sonchus oleraceus (Common sowthistle) [TaxID: 50207]
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=ATCC PV-263;
DOI=10.1016/0042-6822(92)90678-I; PubMed=1604816 [NCBI, ExPASy, EBI, Israel, Japan]
Choi T.-J., Kuwata S., Koonin E.V., Heaton L.A., Jackson A.O.;
"Structure of the L (polymerase) protein gene of sonchus yellow net virus.";
Virology 189:31-39(1992).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein (By similarity).
  • SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the rhabdoviridae protein L family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L32603; AAA50385.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M87829; AAA47896.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A40230; ZLVNSY.
RefSeq NP_042286.1; -.
3D structure databases
ModBase P31332.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P31332.
ProtoNet P31332.
Genome annotation databases
GeneID 1489882; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   2116  2116     Large structural protein. PRO_0000222842
DOMAIN   634    818  185     RdRp catalytic. 
Sequence information
Length: 2116 AA [This is the length of the unprocessed precursor] Molecular weight: 241538 Da [This is the MW of the unprocessed precursor] CRC64: 74D188F3D4CF1D80 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MEGMDHWENA KYFQGIEDIE EDTRQPTVDS MSSGTYHCKS ALRSHKDNMK LFLYRRDFLI 

        70         80         90        100        110        120 
FSHRFNGLPY DEQYLGVLPK LWSCFYDKTH DLSGFLDQYA SREHCTPSDS FSRWADPTVL 

       130        140        150        160        170        180 
HLYDDPIIRN LLASENKVLN FLEGGISDIL DKYQICIKRN IRLIYLHLFL NLALIVLNHT 

       190        200        210        220        230        240 
DADSMPDRRV ELNGVTFKLE EGVILCEYNE YLKIYVLKGA VIWDMPAYRQ VLQKDLFLTI 

       250        260        270        280        290        300 
CDKISERINI VIGATIITAL SHKTNLDDPD SHLYDACINM IKIGDNILVN HGNRGFDLLG 

       310        320        330        340        350        360 
KFEAYCVACI LTYDDQRIWN PLEFLNNLIE DDRINQPDLY NDANNLVAFL RKQPIVILAE 

       370        380        390        400        410        420 
LHGLWRIWGH PIIDLEGGMK KMEATCTKQS PVSVEETRVC ERTMKLTFFT NYYDKHHHYP 

       430        440        450        460        470        480 
LSTLTHPDHF NLYSQYLSER DKIEYLANKD IAFEHSYIMR CIRRNKKIFQ RSSLYNHKDW 

       490        500        510        520        530        540 
DQVVILQSFQ IPKSVNLATM IKDKAISMTR SELIESVNTK NSVFDSTKRR GILKWLNEQS 

       550        560        570        580        590        600 
DKIYNFLMRI DDKGLDEDDC IIGLYPKERE MKTKARFFSL MSYKLRMYVT STEELLGKYV 

       610        620        630        640        650        660 
LKYFPMITMS DNLLSMVIRL FDMTTLIGDK GVAVTYSMNI DFSKWNQNMR ERTNAGIFDN 

       670        680        690        700        710        720 
LDRILGFRSL ISRTHSIFKA CYLYLCSGEY VPVISNNQLT AQSPWSRTGD ESGKEGLRQK 

       730        740        750        760        770        780 
GWTITTVCDI LSLAFKYNAR IQLIGGGDNQ VLTVTMLPSE SMQSQGRDSQ LLKVRERMTS 

       790        800        810        820        830        840 
FRNALAKKMV KRGLPLKLEE TWISHNLLMY NKIMYYSGVP LRGRLKVISR LFSNSNVGVT 

       850        860        870        880        890        900 
SLGGITSTLG TGFQSISTKD YTPTLAWLIS RVFTDIYIST YHLLNPISGT QRLDKQVLMS 

       910        920        930        940        950        960 
RGNIRQGRNE LGGETSVPII NKIRNHAALA TDHTLDLDSL LICVLYYHKI LGGPGIGPPT 

       970        980        990       1000       1010       1020 
AYVMKGFPDP LSEGLTFNYL VITNVLNERT KRKIISVTKV MKNRNQHWEH LLEDPVSVNH 

      1030       1040       1050       1060       1070       1080 
DAPPHGIAAL RAQAEAVMRS AKITNIGFKN LIDIGDNQYL RDLSEKLCSP NDLEPRLLHD 

      1090       1100       1110       1120       1130       1140 
IVGSTIPGFV NSVLSRVDQS TTINKIAGNS DVVTSIYLSE MSYYLYLSKK VNTQDGHAIG 

      1150       1160       1170       1180       1190       1200 
SCPTRDSKML RNWTWGKNII GVTTPHPLGY LKRERHSESS SCDNNYIRVL TKRIGNSWEL 

      1210       1220       1230       1240       1250       1260 
RRGQFRPYFG SYTEEKFKMT TLASAYGDES ILKRAIKIQK LLGWRYHQGS SLYNLIQKIL 

      1270       1280       1290       1300       1310       1320 
TCVTDADPNK FLPLPDEITG DVEHRYHDMA TKHGGIPSNL IHLYTHASCN TSTFINHSKG 

      1330       1340       1350       1360       1370       1380 
AANESLHFQA AIIWTCMQSI CRTSASSSVS DISHYHEACN QCIVKLEDPI ESDYSTSDIS 

      1390       1400       1410       1420       1430       1440 
LMSCPANDLM YVKEDDIPVH FHTTMEFYRA SSSSTVLKKI KKIEDAEVIS SRMTWVVTLC 

      1450       1460       1470       1480       1490       1500 
SHLLNQDTIK HSTWKLISED LSKEEVMFIV MSITMYIMSE QDIPVHSASL SDFRTLYEKN 

      1510       1520       1530       1540       1550       1560 
KDIIDRVLGI EALNDAVSGV SFYNNRCSDD QCLRWKETSD QILSHYKTTG TCAVKYQAPH 

      1570       1580       1590       1600       1610       1620 
FRICTRLVYL MTNPSCQSCP CCLGVIKDDS NDGPIMCQLH GELAGPCGYH LCSLDKLNKT 

      1630       1640       1650       1660       1670       1680 
KKGLNMSKVF YTDGTISAQH DAKNRPTKKR PHGENSLTEM FKRAKTSREN RILKQNKESY 

      1690       1700       1710       1720       1730       1740 
LFIQPRLMVD LFIDMGSMWE KTQISDQGSH IIPAHTNPIV LSKKSDLYVP AAISKFVSNG 

      1750       1760       1770       1780       1790       1800 
FLIMDAVERA LGKPSKPITE HSQLSVNISY GIEYHPEIKR ETVQLLRFVN ELAYTGYGGG 

      1810       1820       1830       1840       1850       1860 
VTICITLFPI FISDIEAVDP RLISDIIYRY RADSSDYACI RLTDMGDMCF DINNILSDCD 

      1870       1880       1890       1900       1910       1920 
ACLSYDPGCW SQDANLVYII SDTSDIMIKA KEFETLHSFN KFYSVECLAP RFFMSSSTVS 

      1930       1940       1950       1960       1970       1980 
ALVISKSSSI NSIDYDRLAE IHLDRRGTQM WDLSRLFANM TMRDGLTEMI KCIYNNVSGE 

      1990       2000       2010       2020       2030       2040 
ATILTSQSLV DAAISKIKVD ILRGLIDMVR GERMNWRTQY MIQILLGVMI LTSDNPEDYR 

      2050       2060       2070       2080       2090       2100 
REISKYNNAV VLLQKPRRIK LIRDHLGGAD RKFYHATTNN GLLGSSLNDV THILEGILYI 

      2110 
THRTSRKLCT SISLEV
P31332 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by au flag APAF Australia Mirror sites: Brazil Canada China Korea Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!