[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Liver; DOI=10.1016/0014-5793(91)80591-P; PubMed=1710579 [NCBI, ExPASy, EBI, Israel, Japan] Onishi Y., Noshiro M., Shimosato T., Okuda K.; "Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver."; FEBS Lett. 283:215-218(1991).
[2]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. TISSUE=Liver; PubMed=1789929 [NCBI, ExPASy, EBI, Israel, Japan] Onishi Y., Noshiro M., Shimosato T., Okuda K.; "Delta 4-3-Oxosteroid 5 beta-reductase. Structure and function."; Biol. Chem. Hoppe-Seyler 372:1039-1049(1991).
[3]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Liver; DOI=10.1053/jhep.1996.v23.pm0008550030; PubMed=8550030 [NCBI, ExPASy, EBI, Israel, Japan] Zhu Y., Fillenwarth M.J., Crabb D., Lumeng L., Lin R.C.; "Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase."; Hepatology 23:115-122(1996).

Comments

FUNCTION: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
CATALYTIC ACTIVITY: 5-beta-cholestan-3-one + NADP⁺ = cholest-4-en-3-one + NADPH.
CATALYTIC ACTIVITY: 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP⁺ = cortisone.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the aldo/keto reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D17309; BAA04131.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S80431; AAB35916.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S15835; S15835.

NP_620239.1; -.

3D structure databases

HSSP

P23457; 1LWI. [HSSP ENTRY / PDB]

ModBase

P31210.

PTM databases

PhosphoSite

P31210; -.

Organism-specific databases

RGD

620752; Akr1d1.

Gene expression databases

ArrayExpress

P31210; -.

GermOnline

ENSRNOG00000013004; Rattus norvegicus.

Ontologies

GO:0047568;	Molecular function: 3-oxo-5-beta-steroid 4-dehydrogenase activity (inferred from direct assay from MGI).
GO:0047787;	Molecular function: delta4-3-oxosteroid 5beta-reductase activity (inferred from electronic annotation from EC).
GO:0030573;	Biological process: bile acid catabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001395; Aldo/ket_red.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.100; Aldo/ket_red; 1.

PANTHER

PTHR11732; Aldo/ket_red; 1.

Pfam

PF00248; Aldo_ket_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00069; ALDKETRDTASE.

ProDom

PD000288; Aldo/ket_red; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00798; ALDOKETO_REDUCTASE_1; 1.
PS00062; ALDOKETO_REDUCTASE_2; 1.
PS00063; ALDOKETO_REDUCTASE_3; 1.

Genome annotation databases

Ensembl

ENSRNOG00000013004; Rattus norvegicus. [Contig view]

GeneID

192242; -.

KEGG

rno:192242; -.

Phylogenomic databases

HOVERGEN

P31210; -.

Other

NextBio

622866; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Bile acid catabolism; Cytoplasm; Direct protein sequencing; Lipid metabolism; NADP; Oxidoreductase; Steroid metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 326`	`326`	`3-oxo-5-beta-steroid 4-dehydrogenase.`	`PRO_0000124672`
`NP_BIND`	`168 169`	`2`	`NADP (By similarity).`
`NP_BIND`	`220 224`	`5`	`NADP (By similarity).`
`NP_BIND`	`273 283`	`11`	`NADP (By similarity).`
`ACT_SITE`	`57 57`		`Proton donor (By similarity).`
`BINDING`	`52 52`		`NADP (By similarity).`
`BINDING`	`192 192`		`NADP (By similarity).`
`BINDING`	`230 230`		`Substrate (By similarity).`

Sequence information

Length: 326 AA [This is the length of the unprocessed precursor]

Molecular weight: 37378 Da [This is the MW of the unprocessed precursor]

CRC64: 32D8266BFA62CE99 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH IDGAYVYRNE 

        70         80         90        100        110        120 
HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA LERTLQTLKL DYIDLYIIEM 

       130        140        150        160        170        180 
PMAFKPGEEF YPKDENGRVI YHKSNLCATW EALEACKDAG LVKSLGVSNF NRRQLEVILN 

       190        200        210        220        230        240 
KPGLKYKPVT NQVECHPYFT QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK 

       250        260        270        280        290        300 
DELLTSLGKK YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE 

       310        320 
ALNKNVRFVE MLMWSDHPEY PFHDEY

P31210 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools