[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=YCH46; DOI=10.1006/bbrc.1994.2269; PubMed=8093075 [NCBI, ExPASy, EBI, Israel, Japan] Akimoto S., Ono T., Tsutsui H., Kinouchi T., Kataoka K., Ohnishi Y.; "Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene."; Biochem. Biophys. Res. Commun. 203:914-921(1994).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=YCH46; Nakayama H., Kuwahara T., Iwasa T., Okamoto S., Tsuchihashi Y., Nakanishi K., Kataoka K., Arimochi H., Ohnishi Y.; "Characterization of a gene cluster for degradation of sialoglycoconjugates in Bacteroides fragilis strain YCH46."; Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=YCH46; DOI=10.1073/pnas.0404172101; PubMed=15466707 [NCBI, ExPASy, EBI, Israel, Japan] Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.; "Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."; Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 235-500. STRAIN=TAL2480; PubMed=2158974 [NCBI, ExPASy, EBI, Israel, Japan] Russo T.A., Thompson J.S., Godoy V.G., Malamy M.H.; "Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli."; J. Bacteriol. 172:2594-2600(1990).

Comments

FUNCTION: Sialidases have been suggested to be pathogenic factors in microbial infections.
CATALYTIC ACTIVITY: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
SUBCELLULAR LOCATION: Periplasm.
SIMILARITY: Belongs to the glycosyl hydrolase 33 family [view classification].
SIMILARITY: Contains 5 BNR repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D28493; BAA05853.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB102772; BAC56895.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP006841; BAD48476.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M31663; AAA22912.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JC2500; JC2500.

RefSeq

YP_099010.1; -.

3D structure databases

HSSP

Q02834; 1EUR. [HSSP ENTRY / PDB]

ModBase

P31206.

Enzyme and pathway databases

BioCyc

BFRA295405:BF1729-MON; -.

Ontologies

GO:0042597;	Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0004308;	Molecular function: exo-alpha-sialidase activity (inferred from electronic annotation from InterPro).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0009405;	Biological process: pathogenesis (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR002860; BNR.
IPR008377; Sialidase_trypan.
Graphical view of domain structure.

Pfam

PF02012; BNR; 5.
Pfam graphical view of domain structure.

PRINTS

PR01803; TCSIALIDASE.

Genome annotation databases

GeneID

3082956; -.

GenomeReviews

AP006841_GR; BF1729.

KEGG

bfr:BF1729; -.

Phylogenomic databases

HOGENOM

P31206; -.

Genome annotation databases

CMR

P31206; BF1729.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Glycosidase; Hydrolase; Periplasm; Repeat; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 22`	`22`	`Potential.`
`CHAIN`	`23 544`	`522`	`Sialidase.`	`PRO_0000012029`
`REPEAT`	`239 250`	`12`	`BNR 1.`
`REPEAT`	`318 329`	`12`	`BNR 2.`
`REPEAT`	`378 389`	`12`	`BNR 3.`
`REPEAT`	`425 436`	`12`	`BNR 4.`
`REPEAT`	`485 496`	`12`	`BNR 5.`
`ACT_SITE`	`399 399`		`Potential.`
`BINDING`	`415 415`		`Substrate (By similarity).`
`BINDING`	`479 479`		`Substrate (By similarity).`
`CONFLICT`	`197 197`		`D -> A (in Ref. 1; BAA05853).`
`CONFLICT`	`405 405`		`V -> A (in Ref. 4; AAA22912).`

Sequence information

Length: 544 AA [This is the length of the unprocessed precursor]

Molecular weight: 59556 Da [This is the MW of the unprocessed precursor]

CRC64: 6D1D185AB15FA822 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKAVILFSL FCFLCAIPVV QAADTIFVRE TRIPILIERQ DNVLFYLRLD AKESQTLNDV 

        70         80         90        100        110        120 
VLNLGEGVNL SEIQSIKLYY GGTEALQDSG KKRFAPVGYI SSNTPGKTLA ANPSYSIKKS 

       130        140        150        160        170        180 
EVTNPGNQVV LKGDQKLFPG INYFWISLQM KPGTSLTSKV TADIASITLD GKKALLDVVS 

       190        200        210        220        230        240 
ENGIEHRMGV GVRHAGDDNS AAFRIPGLVT TNKGTLLGVY DVRYNSSVDL QEHVDVGLSR 

       250        260        270        280        290        300 
STDGGKTWEK MRLPLAFGEF GGLPAGQNGV GDPSILVDTK TNNVWVVAAW THGMGNQRAW 

       310        320        330        340        350        360 
WSSHPGMDMN HTAQLVLAKS TDDGKTWSAP INITEQVKDP SWYFLLQGPG RGITMSDGTL 

       370        380        390        400        410        420 
VFPTQFIDST RVPNAGIMYS KDGGKNWKMH NYARTNTTEA QVAEVEPGVL MLNMRDNRGG 

       430        440        450        460        470        480 
SRAVAITKDL GKTWTEHESS RKALPESVCM ASLISVKAKD NVLGKDLLIF SNPNTTKGRY 

       490        500        510        520        530        540 
NTTIKISLDG GVTWSPEHQL LLDEGNNWGY SCLSMIDKET IGILYESSVA HMTFQAVKLK 


DIIK

P31206 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools