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- FUNCTION: Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
- CATALYTIC ACTIVITY: Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met-|-Arg-Phe-Ala.
- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
- SUBCELLULAR LOCATION: Periplasm. Secreted, cell wall. Note=Stored in the periplasm of gametes until its release. Secreted concurrently with release of the cell walls.
- INDUCTION: By the signal of flagellar agglutination between gametes of the opposite mating type.
- DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
- PTM: Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions.
- SIMILARITY: Belongs to the peptidase M11 family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 638 AA [This is the length of the unprocessed precursor] |
Molecular weight: 69833 Da [This is the MW of the unprocessed precursor] |
CRC64: D3E3467701177251 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSLATRRFGA AAALLVAACV LCTAPAWAQN ETTGTGMVKT KSAFRWIRPP PARPPPFRRP
70 80 90 100 110 120
PPAQTPYVHK VEYTELQILC PQTIDSVTGY PMDDPRCNVP RATVAAGEEA LTIRNEFELL
130 140 150 160 170 180
NGDVLNVTLE EVDTPENPSR RRLLSIIREE QRTGRVLLAT SAELPTPTFR LKSLKSILKG
190 200 210 220 230 240
SQKEIYAGKP IDLRTIVYIM DFSSCKLSGW SAPATLTPEK VTSDMLRGAS APTNNLANYY
250 260 270 280 290 300
GACSYEKTLF NPDNFLVLGP VPVPCIGGVT PPPRPPRPPR PPPRAGSTIS SLSRRNDTYD
310 320 330 340 350 360
DWWDLSKYCT ASEQQAWERA AEAYAQAIVA QDPNSATGKK LQGILQWRER RRNIYILPPG
370 380 390 400 410 420
VKCSWSGYAD VTCTSATCSA YVRGYSDTNA MQVIMHEAMH NYGLEHAGRG TLEYGDATDV
430 440 450 460 470 480
MGDFNKAGKG LLCPNAPNMY RIGWAKPINE PGVAPFQNAT GAWGNLTAAN FTTDPWIRGL
490 500 510 520 530 540
VIPAQGTRDD NMIVVNVGAQ STRDGAMKAT GAQAYYFSYR IKNTTAGGYD SGLTLDFHKK
550 560 570 580 590 600
VLVHAYNGIQ SERVFGFKSN LLDWGPNFQS RSNTWTSPFL AYNNGLGGGV RLVVQSTSDT
610 620 630
QAVVDICRIS ENGKELSCDD GIDNDCDGLQ DNEDPDCQ
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P31178 in FASTA format |
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