ID   6PGD_TRYBB              Reviewed;         479 AA.
AC   P31072;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 60.
DE   RecName: Full=6-phosphogluconate dehydrogenase, decarboxylating;
DE            EC=1.1.1.44;
GN   Name=GND;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=427;
RX   MEDLINE=93149212; PubMed=8426618; DOI=10.1016/0166-6851(93)90247-U;
RA   Barrett M.P., le Page R.W.F.;
RT   "A 6-phosphogluconate dehydrogenase gene from Trypanosoma brucei.";
RL   Mol. Biochem. Parasitol. 57:89-100(1993).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
RX   MEDLINE=98411456; PubMed=9737929; DOI=10.1006/jmbi.1998.2059;
RA   Phillips C., Dohnalek J., Gover S., Barrett M.P., Adams M.J.;
RT   "A 2.8-A resolution structure of 6-phosphogluconate dehydrogenase from
RT   the protozoan parasite Trypanosoma brucei: comparison with the sheep
RT   enzyme accounts for differences in activity with coenzyme and
RT   substrate analogues.";
RL   J. Mol. Biol. 282:667-681(1998).
CC   -!- CATALYTIC ACTIVITY: 6-phospho-D-gluconate + NADP(+) = D-ribulose
CC       5-phosphate + CO(2) + NADPH.
CC   -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-
CC       ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage):
CC       step 3/3.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the 6-phosphogluconate dehydrogenase
CC       family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X65623; CAA46577.1; -; Genomic_DNA.
DR   PIR; A48565; A48565.
DR   PDB; 1PGJ; X-ray; 2.82 A; A/B=2-479.
DR   PDBsum; 1PGJ; -.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0004616; F:phosphogluconate dehydrogenase (decarboxyla...; IEA:InterPro.
DR   GO; GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
DR   InterPro; IPR006183; 6-phosphogluconate_DHase.
DR   InterPro; IPR006114; 6PGDH_C.
DR   InterPro; IPR006113; 6PGDH_decarbox.
DR   InterPro; IPR006115; 6PGDH_NAD-bd.
DR   InterPro; IPR006184; 6PGdom_BS.
DR   InterPro; IPR013328; DHase_multihelical.
DR   InterPro; IPR012284; Fibritin/6PGD_C-extension.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:1.20.5.320; Fibritin/6PGD_C-extension; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF00393; 6PGD; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PRINTS; PR00076; 6PGDHDRGNASE.
DR   TIGRFAMs; TIGR00873; gnd; 1.
DR   PROSITE; PS00461; 6PGD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Gluconate utilization; NADP; Oxidoreductase;
KW   Pentose shunt.
FT   CHAIN         1    479       6-phosphogluconate dehydrogenase,
FT                                decarboxylating.
FT                                /FTId=PRO_0000090071.
FT   STRAND        3      8
FT   HELIX        12     23
FT   STRAND       28     31
FT   HELIX        35     44
FT   TURN         45     47
FT   HELIX        51     53
FT   STRAND       54     56
FT   HELIX        60     66
FT   STRAND       72     75
FT   HELIX        81     93
FT   STRAND       99    102
FT   HELIX       108    119
FT   TURN        120    122
FT   STRAND      124    132
FT   HELIX       133    139
FT   STRAND      142    147
FT   HELIX       149    162
FT   HELIX       181    209
FT   HELIX       214    226
FT   HELIX       233    243
FT   STRAND      249    251
FT   HELIX       252    255
FT   HELIX       265    276
FT   HELIX       281    294
FT   HELIX       296    305
FT   TURN        307    310
FT   HELIX       325    356
FT   HELIX       362    367
FT   STRAND      370    373
FT   HELIX       380    389
FT   HELIX       398    400
FT   HELIX       401    421
FT   HELIX       427    439
FT   HELIX       446    458
FT   STRAND      462    471
SQ   SEQUENCE   479 AA;  52154 MW;  64FED260915ABC2F CRC64;
     MSMDVGVVGL GVMGANLALN IAEKGFKVAV FNRTYSKSEE FMKANASAPF AGNLKAFETM
     EAFAASLKKP RKALILVQAG AATDSTTEQL KKVFEKGDIL VDTGNAHFKD QGRRAQQLEA
     AGLRFLGMGI SGGEEGARKG PAFFPGGTLS VWEEIRPIVE AAAAKADDGR PCVTMNGSGG
     AGSCVKMYHN SGEYAILQIW GEVFDILRAM GLNNDEVAAV LEDWKSKNFL KSYMLDISIA
     AARAKDKDGS YLTEHVMDRI GSKGTGLWSA QEALEIGVPA PSLNMAVVSR QFTMYKTERQ
     ANASNAPGIT QSPGYTLKNK SPSGPEIKQL YDSVCIAIIS CYAQMFQCLR EMDKVHNFGL
     NLPATIATFR AGCILQGYLL KPMTEAFEKN PNISNLMCAF QTEIRAGLQN YRDMVALITS
     KLEVSIPVLS ASLNYVTAMF TPTLKYGQLV SLQRDVFGRH GYERVDKDGR ESFQWPELQ
//