[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=MS11; PubMed=1321116 [NCBI, ExPASy, EBI, Israel, Japan] Stein D.C., Chien R., Seifert H.S.; "Construction of a Neisseria gonorrhoeae MS11 derivative deficient in NgoMI restriction and modification."; J. Bacteriol. 174:4899-4906(1992).

Comments

FUNCTION: This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SIMILARITY: Belongs to the C5-methyltransferase family.
CAUTION: Was originally (Ref.1) known as M.nGmomI.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M86915; -; NOT_ANNOTATED_CDS; Genomic_DNA.

[EMBL / GenBank / DDBJ]

PIR

A42709; A42709.

3D structure databases

HSSP

P20589; 1DCT. [HSSP ENTRY / PDB]

ModBase

P31033.

Protein family/group databases

REBASE

3897; M.MjaV.

Ontologies

GO:0003886;	Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0006306;	Biological process: DNA methylation (inferred from electronic annotation from InterPro).
GO:0009307;	Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001525; C5_DNA_meth.
Graphical view of domain structure.

PANTHER

PTHR10629; C5_DNA_meth; 1.

Pfam

PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00105; C5METTRFRASE.

TIGRFAMs

TIGR00675; dcm; 1.

PROSITE

PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 312`	`312`	`Modification methylase NgoMIV.`	`PRO_0000087899`
`ACT_SITE`	`74 74`		`By similarity.`

Sequence information

Length: 312 AA [This is the length of the unprocessed precursor]

Molecular weight: 35226 Da [This is the MW of the unprocessed precursor]

CRC64: 319E20242B873452 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQFTSLEICA GAGGQALGLE RAGFSHVALI EIEPSACQTL RLNRPDWNVI EGDVRLFQGE 

        70         80         90        100        110        120 
GYDGIDLLAG GVPCPPFSKA GKQLGKDDER DLFPEAIRLA KETDPKAIML ENVRGLLDPK 

       130        140        150        160        170        180 
FENYRNHITE QFAKLGYLGQ WKLLYAADYG VSQLRPRVLF VALKNEYTNF FKWPEPNSEQ 

       190        200        210        220        230        240 
PKTVGELLFD LMSENNWQGA HNWRLKAAQI APTLVAVQKN TAVLTWDLHD PNAHGRSWVW 

       250        260        270        280        290        300 
MVQVCGIVRR LKTFTGMPRL TVRMTARIQG FPDDWQFFGK KTPMYRQIGN AFPPPVAEAV 

       310 
GRQIIKALKK EN

P31033 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools