ID   MASY_YEAST              Reviewed;         554 AA.
AC   P30952;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 73.
DE   RecName: Full=Malate synthase 1, glyoxysomal;
DE            EC=2.3.3.9;
GN   Name=MLS1; OrderedLocusNames=YNL117W; ORFNames=N1921;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93087177; PubMed=1454530; DOI=10.1093/nar/20.21.5677;
RA   Hartig A., Simon M.M., Schuster T., Daugherty J.R., Yoo H.S.,
RA   Cooper T.G.;
RT   "Differentially regulated malate synthase genes participate in carbon
RT   and nitrogen metabolism of S. cerevisiae.";
RL   Nucleic Acids Res. 20:5677-5686(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97245296; PubMed=9090055;
RX   DOI=10.1002/(SICI)1097-0061(19970315)13:3<261::AID-YEA64>3.0.CO;2-L;
RA   de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D.,
RA   Pallavicini A., Lanfranchi G., Valle G.;
RT   "The DNA sequence of cosmid 14-13b from chromosome XIV of
RT   Saccharomyces cerevisiae reveals an unusually high number of
RT   overlapping open reading frames.";
RL   Yeast 13:261-266(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313269; PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F.,
RA   Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M.,
RA   Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N.,
RA   Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D.,
RA   Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A.,
RA   Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A.,
RA   Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C.,
RA   Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M.,
RA   Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J.,
RA   Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L.,
RA   Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M.,
RA   Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P.,
RA   Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A.,
RA   Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV
RT   and its evolutionary implications.";
RL   Nature 387:93-98(1997).
CC   -!- FUNCTION: This isozyme is necessary for growth on acetate as sole
CC       C-source.
CC   -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + glyoxylate = (S)-malate +
CC       CoA.
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; L-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- INTERACTION:
CC       P43582:-; NbExp=1; IntAct=EBI-10428, EBI-22766;
CC       Q08409:AUS1; NbExp=1; IntAct=EBI-10428, EBI-35723;
CC       P22696:ESS1; NbExp=1; IntAct=EBI-10428, EBI-6679;
CC       P33203:PRP40; NbExp=1; IntAct=EBI-10428, EBI-701;
CC       P39940:RSP5; NbExp=1; IntAct=EBI-10428, EBI-16219;
CC       P40318:SSM4; NbExp=1; IntAct=EBI-10428, EBI-18208;
CC       Q06525:URN1; NbExp=1; IntAct=EBI-10428, EBI-35138;
CC       P53076:VID30; NbExp=1; IntAct=EBI-10428, EBI-24173;
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- SIMILARITY: Belongs to the malate synthase family.
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DR   EMBL; X64407; CAA45750.1; -; Genomic_DNA.
DR   EMBL; Z69382; CAA93390.1; -; Genomic_DNA.
DR   EMBL; Z71393; CAA95997.1; -; Genomic_DNA.
DR   PIR; S26645; S26645.
DR   RefSeq; NP_014282.1; -.
DR   IntAct; P30952; -.
DR   PeptideAtlas; P30952; -.
DR   Ensembl; YNL117W; Saccharomyces cerevisiae.
DR   GeneID; 855606; -.
DR   KEGG; sce:YNL117W; -.
DR   NMPDR; fig|4932.3.peg.5352; -.
DR   CYGD; YNL117w; -.
DR   SGD; S000005061; MLS1.
DR   HOGENOM; P30952; -.
DR   LinkHub; P30952; -.
DR   NextBio; 979777; -.
DR   ArrayExpress; P30952; -.
DR   GermOnline; YNL117W; Saccharomyces cerevisiae.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-KW.
DR   GO; GO:0004474; F:malate synthase activity; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR006252; Malate_synthA.
DR   InterPro; IPR001465; Malate_synthase.
DR   PANTHER; PTHR21631:SF1; Malate_synthase; 1.
DR   Pfam; PF01274; Malate_synthase; 1.
DR   PIRSF; PIRSF001363; Malate_synth; 1.
DR   TIGRFAMs; TIGR01344; malate_syn_A; 1.
DR   PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Glyoxylate bypass; Glyoxysome; Transferase;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    554       Malate synthase 1, glyoxysomal.
FT                                /FTId=PRO_0000166864.
FT   MOTIF       552    554       Microbody targeting signal (Potential).
FT   ACT_SITE    177    177       Proton acceptor (By similarity).
FT   ACT_SITE    457    457       Proton donor (By similarity).
SQ   SEQUENCE   554 AA;  62791 MW;  98E698E86E59C480 CRC64;
     MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV
     VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV
     NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG
     WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF
     CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN
     DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK
     VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN
     GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD
     TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE
     IVSTKATPTD LSKL
//