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UniProtKB/Swiss-Prot entry P30952


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MASY_YEAST
Primary accession number P30952
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on July 1, 1993 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 71)
Name and origin of the protein
Protein name Malate synthase 1, glyoxysomal
Synonym EC 2.3.3.9
Gene name
Name: MLS1
OrderedLocusNames: YNL117W
ORFNames: N1921
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1093/nar/20.21.5677; PubMed=1454530 [NCBI, ExPASy, EBI, Israel, Japan]
Hartig A., Simon M.M., Schuster T., Daugherty J.R., Yoo H.S., Cooper T.G.;
"Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S. cerevisiae.";
Nucleic Acids Res. 20:5677-5686(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1002/(SICI)1097-0061(19970315)13:3<261::AID-YEA64>3.0.CO;2-L; PubMed=9090055 [NCBI, ExPASy, EBI, Israel, Japan]
de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.;
"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames.";
Yeast 13:261-266(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169873 [NCBI, ExPASy, EBI, Israel, Japan]
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.";
Nature 387:93-98(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X64407; CAA45750.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z69382; CAA93390.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z71393; CAA95997.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S26645; S26645.
RefSeq NP_014282.1; -.
3D structure databases
ModBase P30952.
Protein-protein interaction databases
IntAct P30952; -.
Organism-specific databases
CYGD YNL117w; -.
SGD S000005061; MLS1.
Yeast-GFP YNL117W.
Gene expression databases
ArrayExpress P30952; -.
GermOnline YNL117W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR006252; Malate_synthA.
IPR001465; Malate_synthase.
Graphical view of domain structure.
PANTHER PTHR21631:SF1; Malate_synthase; 1.
Pfam PF01274; Malate_synthase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001363; Malate_synth; 1.
TIGRFAMs TIGR01344; malate_syn_A; 1.
PROSITE PS00510; MALATE_SYNTHASE; 1.
BLOCKS P30952.
Proteomic databases
PeptideAtlas P30952; -.
Genome annotation databases
Ensembl YNL117W; Saccharomyces cerevisiae. [Contig view]
GeneID 855606; -.
KEGG sce:YNL117W; -.
NMPDR fig|4932.3.peg.5352; -.
Phylogenomic databases
HOGENOM P30952; -.
Other
LinkHub P30952; -.
ProtoNet P30952.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glyoxylate bypass; Glyoxysome; Transferase; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   554  554     Malate synthase 1, glyoxysomal. PRO_0000166864
MOTIF   552   554  3     Microbody targeting signal (Potential). 
ACT_SITE   177   177        Proton acceptor (By similarity). 
ACT_SITE   457   457        Proton donor (By similarity). 
Sequence information
Length: 554 AA [This is the length of the unprocessed precursor] Molecular weight: 62791 Da [This is the MW of the unprocessed precursor] CRC64: 98E698E86E59C480 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVKVSLDNVK LLVDVDKEPF FKPSSTTVGD ILTKDALEFI VLLHRTFNNK RKQLLENRQV 

        70         80         90        100        110        120 
VQKKLDSGSY HLDFLPETAN IRNDPTWQGP ILAPGLINRS TEITGPPLRN MLINALNAPV 

       130        140        150        160        170        180 
NTYMTDFEDS ASPTWNNMVY GQVNLYDAIR NQIDFDTPRK SYKLNGNVAN LPTIIVRPRG 

       190        200        210        220        230        240 
WHMVEKHLYV DDEPISASIF DFGLYFYHNA KELIKLGKGP YFYLPKMEHH LEAKLWNDVF 

       250        260        270        280        290        300 
CVAQDYIGIP RGTIRATVLI ETLPAAFQME EIIYQLRQHS SGLNCGRWDY IFSTIKRLRN 

       310        320        330        340        350        360 
DPNHILPNRN QVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PAANEKAMTK 

       370        380        390        400        410        420 
VRNDKIRELT NGHDGSWVAH PALAPICNEV FINMGTPNQI YFIPENVVTA ANLLETKIPN 

       430        440        450        460        470        480 
GEITTEGIVQ NLDIGLQYME AWLRGSGCVP INNLMEDAAT AEVSRCQLYQ WVKHGVTLKD 

       490        500        510        520        530        540 
TGEKVTPELT EKILKEQVER LSKASPLGDK NKFALAAKYF LPEIRGEKFS EFLTTLLYDE 

       550 
IVSTKATPTD LSKL 

P30952 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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