ID   L_MUMPM                 Reviewed;        2261 AA.
AC   P30929;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 49.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Mumps virus (strain Miyahara vaccine).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Paramyxoviridae; Paramyxovirinae; Rubulavirus.
OX   NCBI_TaxID=11171;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=92263804; PubMed=1585659; DOI=10.1016/0042-6822(92)90555-4;
RA   Okazaki K., Tanabayashi K., Takeuchi K., Hishiyama M., Okazaki K.,
RA   Yamada A.;
RT   "Molecular cloning and sequence analysis of the mumps virus gene
RT   encoding the L protein and the trailer sequence.";
RL   Virology 188:926-930(1992).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently the subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The transcriptase
CC       stutters on a specific sequence, resulting on a cotranscriptional
CC       editing of the phosphoprotein (P) mRNA. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the paramyxoviruses L protein family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; D10575; BAA01432.1; -; Genomic_RNA.
DR   PIR; A42548; A42548.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR016269; RNA-dir_RNA_pol_paramyxovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF000830; RNA_pol_ParamyxoV; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   2261       Large structural protein.
FT                                /FTId=PRO_0000142730.
FT   DOMAIN      662    846       RdRp catalytic.
FT   NP_BIND    1811   1820       ATP (Potential).
SQ   SEQUENCE   2261 AA;  256575 MW;  A789F54BFC1EB5EB CRC64;
     MAGLNEILLP EVHLNSPIVR YKLFYYILHG QLPNDLEPDD LGPLANQNWK AIRAEESQVH
     ARLKQIRVEL IARIPSLRWT RSQREIAILI WPRILPILQA YDLRQSMQLP TVWEKLTQST
     VNLISDGLER VVLHISNQLT GKPNLFTRSR AGQDVKDYSI PSTRELSQIW FNNEWSGSVK
     TWLMIKYRMR QLITNQKTGE LTDLVTIVDT RSTLCIIAPE LVALYSNEHK ALTYLTFEMV
     LMVTDMLEGR LNVSSLCTAS HYLSPLKKRI EILLTLVDDL ALLMGDKVYG VVSSLESFVY
     AQLQYGDPVV DIKGTFYGFI CNEILDLLTE DNIFTEEEAN KVLLDLTSQF DNLSPDLTAE
     LLCIMRLWGH PTLTASQAAS KVRESMCAPK VLDFQTIMKT LAFFHAILIN GYRRSHNGIW
     PPTTLHGNAP KSLIEMRHDN SELKYEYVLK NWKSISMLRI HKCFDASPDE DLSIFMKDKA
     ISCPKQDWMG VFRRSLIKQR YRDANRPLPQ PFNRRLLLNF LEDDRFDPIK ELEYVTSGEY
     LRDPEFCASY SLKEKEIKAT GRIFAKMTKR MRSCQVIAES LLANHAGKLM RENGVVLDQL
     KLTKSLLTMN QIGIISEHSR RSTADNMTLA HSGSNKHRIN NSQFKKNKDS KHEMPDDGFE
     IAACFLTTDL TKYCLNWRYQ VIIPFARTLN SMYGIPHLFE WIHLRLMRST LYVGDPFNPP
     SDPTQLDLDT ALNDDIFIVS PRGGIEGLCQ KLWTMISIST IILSATEANT RVMSMVQGDN
     QAIAITTRVV RSLSHSEKKE QAYKASKLFF ERLRANNHGI GHHLKEQETI LSSDFFIYSK
     RVFYKGRILT QALKNVSKMC LTADILGDCS QASCSNLATT VMRLTENGVE KDLCYFLNAF
     MTIRQLCYDL VFPQTKSLSQ DITNAYLNHP ILISRLCLLP SQLGGLNFLS CSRLFNRNIG
     DPLVSAIADV KRLIKAGCLD IWVLYNILGR RPGKGKWSTL AADPYTLNID YLVPSKTFLK
     KHAQYTLMER SVNPMLRGVF SENAAEEEEE LAQYLLDREV VMPRVAHVIL AQSSCGRRKQ
     IQGYLDSTRT IIRYSLEVRP LSAKKLNTVI EYNLLYLSYN LEIIEKPNIV QPFLNAINVD
     TCSIDIARSL RKLSWATLLN GRPIEGLETP DPIELVHGCL IIGSDECEHC SSGDDKFTWF
     FLPKGIRLDN DPASNPPIRV PYIGSKTDER RVASMAYIKG ASVSLKSALR LAGVYIWAFG
     DTEESWQDAY ELASTRVNLT LEQLQSLTPL PTSANLVHRL DDGTTQLKFT PASSYAFSSF
     VHISNDCQVL EIDDQVTDSN LIYQQVMITG LALIETWNNP PINFSVYETT LHLHTGSSCC
     IRPVESCVVN PPLLPVPFIN VPQMNKFVYD PEPLSLLEME KIEDIAYQTR IGGLDQIPLL
     EKIPLLAHLT AKQMVNSITG LDEATSIVND AVVQADYTSN WISECCYTYI DSVFVYSGWA
     LLLELSYQMY YLRIQGIQGI LDYVYMTLRR IPGMAITGIS STISHPRILR RCINLDVIAP
     INSPHIASLD YTKLSIDAVM WGTKQVLTNI SQGIDYEIVV PSESQLTLSD RVLNLVARKL
     SLLAIIWANY NYPPKVKGMS PEDKCQALTT HLLQTVEYVE HIQIEKTNIR RMIIEPKLTA
     YPSNLFYLSR KLLNAIRDSE EGQFLIASYY NSFGYLEPIL MESKIFNLSS SESASLTEFD
     FILNLELSEA SLEKYSLPSL LMTAENMDNP FPQPPLHHVL RPLGLSSTSW YKTISVLNYI
     SHMKISDGAH LYLAEGSGAS MSLIETFLPG EIIWYNSLFN SGENPPQRNF APLPTQFIES
     VPYRLIQAGI AAGSGVVQSF YPLWNGNSDI TDLSTKTSVE YIIHKVGADT CALVHVDLEG
     VPGSMNSMLE RAQVHALLIT VTVLKPGGLL ILKASWEPFN RFSFLLTILW QFSSTIRILR
     SSYSDPNNHE VYIIATLAVD PTTSSFTTAL NRARTLNEQG FSLIPPELVS EYWRRRVEQG
     QIIQDCIDKV ISECVRDQYL ADNNIILQAG GTPSTRKWLD LPDYLSFNEL QSEMARLITI
     HLKEVIEILK GQSSDHDTLL FTSYNVGPLG KINTILRLIV ERILMYTVRN WCILPTQTRL
     TLRQSIELGE FRLRDVITPM EILKLSPNRK YLKSALNQST FNHLMGETSD ILLNRSYQKR
     IWKAIGCVIY CFGLLTPDVE DSERIDIDND IPDYDIHGDI I
//