RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Mumps virus (strain Miyahara vaccine)

[TaxID: 11171]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Rubulavirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(92)90555-4; PubMed=1585659 [NCBI, ExPASy, EBI, Israel, Japan]
Okazaki K., Tanabayashi K., Takeuchi K., Hishiyama M., Okazaki K., Yamada A.;
"Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence.";
Virology 188:926-930(1992).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D10575; BAA01432.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A42548; A42548.

3D structure databases

ModBase

P30929.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2261`	`2261`	`Large structural protein.`	`PRO_0000142730`
`DOMAIN`	`662 846`	`185`	`RdRp catalytic.`
`NP_BIND`	`1811 1820`	`10`	`ATP (Potential).`

Sequence information

Length: 2261 AA [This is the length of the unprocessed precursor]

Molecular weight: 256575 Da [This is the MW of the unprocessed precursor]

CRC64: A789F54BFC1EB5EB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAGLNEILLP EVHLNSPIVR YKLFYYILHG QLPNDLEPDD LGPLANQNWK AIRAEESQVH 

        70         80         90        100        110        120 
ARLKQIRVEL IARIPSLRWT RSQREIAILI WPRILPILQA YDLRQSMQLP TVWEKLTQST 

       130        140        150        160        170        180 
VNLISDGLER VVLHISNQLT GKPNLFTRSR AGQDVKDYSI PSTRELSQIW FNNEWSGSVK 

       190        200        210        220        230        240 
TWLMIKYRMR QLITNQKTGE LTDLVTIVDT RSTLCIIAPE LVALYSNEHK ALTYLTFEMV 

       250        260        270        280        290        300 
LMVTDMLEGR LNVSSLCTAS HYLSPLKKRI EILLTLVDDL ALLMGDKVYG VVSSLESFVY 

       310        320        330        340        350        360 
AQLQYGDPVV DIKGTFYGFI CNEILDLLTE DNIFTEEEAN KVLLDLTSQF DNLSPDLTAE 

       370        380        390        400        410        420 
LLCIMRLWGH PTLTASQAAS KVRESMCAPK VLDFQTIMKT LAFFHAILIN GYRRSHNGIW 

       430        440        450        460        470        480 
PPTTLHGNAP KSLIEMRHDN SELKYEYVLK NWKSISMLRI HKCFDASPDE DLSIFMKDKA 

       490        500        510        520        530        540 
ISCPKQDWMG VFRRSLIKQR YRDANRPLPQ PFNRRLLLNF LEDDRFDPIK ELEYVTSGEY 

       550        560        570        580        590        600 
LRDPEFCASY SLKEKEIKAT GRIFAKMTKR MRSCQVIAES LLANHAGKLM RENGVVLDQL 

       610        620        630        640        650        660 
KLTKSLLTMN QIGIISEHSR RSTADNMTLA HSGSNKHRIN NSQFKKNKDS KHEMPDDGFE 

       670        680        690        700        710        720 
IAACFLTTDL TKYCLNWRYQ VIIPFARTLN SMYGIPHLFE WIHLRLMRST LYVGDPFNPP 

       730        740        750        760        770        780 
SDPTQLDLDT ALNDDIFIVS PRGGIEGLCQ KLWTMISIST IILSATEANT RVMSMVQGDN 

       790        800        810        820        830        840 
QAIAITTRVV RSLSHSEKKE QAYKASKLFF ERLRANNHGI GHHLKEQETI LSSDFFIYSK 

       850        860        870        880        890        900 
RVFYKGRILT QALKNVSKMC LTADILGDCS QASCSNLATT VMRLTENGVE KDLCYFLNAF 

       910        920        930        940        950        960 
MTIRQLCYDL VFPQTKSLSQ DITNAYLNHP ILISRLCLLP SQLGGLNFLS CSRLFNRNIG 

       970        980        990       1000       1010       1020 
DPLVSAIADV KRLIKAGCLD IWVLYNILGR RPGKGKWSTL AADPYTLNID YLVPSKTFLK 

      1030       1040       1050       1060       1070       1080 
KHAQYTLMER SVNPMLRGVF SENAAEEEEE LAQYLLDREV VMPRVAHVIL AQSSCGRRKQ 

      1090       1100       1110       1120       1130       1140 
IQGYLDSTRT IIRYSLEVRP LSAKKLNTVI EYNLLYLSYN LEIIEKPNIV QPFLNAINVD 

      1150       1160       1170       1180       1190       1200 
TCSIDIARSL RKLSWATLLN GRPIEGLETP DPIELVHGCL IIGSDECEHC SSGDDKFTWF 

      1210       1220       1230       1240       1250       1260 
FLPKGIRLDN DPASNPPIRV PYIGSKTDER RVASMAYIKG ASVSLKSALR LAGVYIWAFG 

      1270       1280       1290       1300       1310       1320 
DTEESWQDAY ELASTRVNLT LEQLQSLTPL PTSANLVHRL DDGTTQLKFT PASSYAFSSF 

      1330       1340       1350       1360       1370       1380 
VHISNDCQVL EIDDQVTDSN LIYQQVMITG LALIETWNNP PINFSVYETT LHLHTGSSCC 

      1390       1400       1410       1420       1430       1440 
IRPVESCVVN PPLLPVPFIN VPQMNKFVYD PEPLSLLEME KIEDIAYQTR IGGLDQIPLL 

      1450       1460       1470       1480       1490       1500 
EKIPLLAHLT AKQMVNSITG LDEATSIVND AVVQADYTSN WISECCYTYI DSVFVYSGWA 

      1510       1520       1530       1540       1550       1560 
LLLELSYQMY YLRIQGIQGI LDYVYMTLRR IPGMAITGIS STISHPRILR RCINLDVIAP 

      1570       1580       1590       1600       1610       1620 
INSPHIASLD YTKLSIDAVM WGTKQVLTNI SQGIDYEIVV PSESQLTLSD RVLNLVARKL 

      1630       1640       1650       1660       1670       1680 
SLLAIIWANY NYPPKVKGMS PEDKCQALTT HLLQTVEYVE HIQIEKTNIR RMIIEPKLTA 

      1690       1700       1710       1720       1730       1740 
YPSNLFYLSR KLLNAIRDSE EGQFLIASYY NSFGYLEPIL MESKIFNLSS SESASLTEFD 

      1750       1760       1770       1780       1790       1800 
FILNLELSEA SLEKYSLPSL LMTAENMDNP FPQPPLHHVL RPLGLSSTSW YKTISVLNYI 

      1810       1820       1830       1840       1850       1860 
SHMKISDGAH LYLAEGSGAS MSLIETFLPG EIIWYNSLFN SGENPPQRNF APLPTQFIES 

      1870       1880       1890       1900       1910       1920 
VPYRLIQAGI AAGSGVVQSF YPLWNGNSDI TDLSTKTSVE YIIHKVGADT CALVHVDLEG 

      1930       1940       1950       1960       1970       1980 
VPGSMNSMLE RAQVHALLIT VTVLKPGGLL ILKASWEPFN RFSFLLTILW QFSSTIRILR 

      1990       2000       2010       2020       2030       2040 
SSYSDPNNHE VYIIATLAVD PTTSSFTTAL NRARTLNEQG FSLIPPELVS EYWRRRVEQG 

      2050       2060       2070       2080       2090       2100 
QIIQDCIDKV ISECVRDQYL ADNNIILQAG GTPSTRKWLD LPDYLSFNEL QSEMARLITI 

      2110       2120       2130       2140       2150       2160 
HLKEVIEILK GQSSDHDTLL FTSYNVGPLG KINTILRLIV ERILMYTVRN WCILPTQTRL 

      2170       2180       2190       2200       2210       2220 
TLRQSIELGE FRLRDVITPM EILKLSPNRK YLKSALNQST FNHLMGETSD ILLNRSYQKR 

      2230       2240       2250       2260 
IWKAIGCVIY CFGLLTPDVE DSERIDIDND IPDYDIHGDI I

P30929 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools