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UniProtKB/Swiss-Prot entry P30919

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ASPG_RAT
Primary accession number P30919
Secondary accession number Q4G065
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on December 6, 2005 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 62)
Name and origin of the protein
Protein name N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase [Precursor]
Synonyms EC 3.5.1.26
Glycosylasparaginase
Aspartylglucosaminidase
AGA
N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase
Contains Glycosylasparaginase alpha chain
Glycosylasparaginase beta chain
Gene name
Name: Aga
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Spleen;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[2]
 PROTEIN SEQUENCE OF 24-41 AND 205-226.
TISSUE=Liver;
PubMed=2775174 [NCBI, ExPASy, EBI, Israel, Japan]
Tollersrud O.-K., Aronson N.N. Jr.;
"Purification and characterization of rat liver glycosylasparaginase.";
Biochem. J. 260:101-108(1989).
[3]
 SEQUENCE REVISION.
TISSUE=Liver;
PubMed=1554372 [NCBI, ExPASy, EBI, Israel, Japan]
Tollersrud O.-K., Aronson N.N. Jr.;
"Comparison of liver glycosylasparaginases from six vertebrates.";
Biochem. J. 282:891-897(1992).
Comments
  • FUNCTION: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.
  • CATALYTIC ACTIVITY: N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate.
  • SUBUNIT: Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.
  • SUBCELLULAR LOCATION: Lysosome.
  • SIMILARITY: Belongs to the Ntn-hydrolase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BC098718; AAH98718.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S04228; S04228.
S04229; S04229.
S57865; S57865.
RefSeq NP_001026811.1; -.
UniGene Rn.104649
3D structure databases
HSSP P20933; 1APY. [HSSP ENTRY / PDB]
SMR P30919; 25-185, 205-345.
ModBase P30919.
Protein family/group databases
MEROPS T02.001; -.
Organism-specific databases
RGD 1309646; Aga.
Gene expression databases
ArrayExpress P30919; -.
GermOnline ENSRNOG00000000108; Rattus norvegicus.
Ontologies
GO
GO:0005764; Cellular component: lysosome (inferred from electronic annotation from UniProtKB-KW).
GO:0003948; Molecular function: N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR000246; Peptidase_T2.
Graphical view of domain structure.
PANTHER PTHR10188; Peptidase_T2; 1.
Pfam PF01112; Asparaginase_2; 1.
Pfam graphical view of domain structure.
BLOCKS P30919.
ProtoNet P30919.
Genome annotation databases
Ensembl ENSRNOG00000000108; Rattus norvegicus. [Contig view]
GeneID 290923; -.
KEGG rno:290923; -.
Phylogenomic databases
HOVERGEN P30919; -.
Other
NextBio 631851; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Glycoprotein; Hydrolase; Lysosome; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    23  23      
CHAIN   24   204  181     Glycosylasparaginase alpha chain. PRO_0000044571
CHAIN   205   345  141     Glycosylasparaginase beta chain. PRO_0000044572
ACT_SITE   205   205        Nucleophile (By similarity). 
CARBOHYD   38    38        N-linked (GlcNAc...) (Potential). 
CARBOHYD   149   149        N-linked (GlcNAc...) (Potential). 
CARBOHYD   307   307        N-linked (GlcNAc...) (Potential). 
DISULFID   64    69        By similarity. 
DISULFID   163   179        By similarity. 
DISULFID   285   305        By similarity. 
DISULFID   316   344        By similarity. 
Sequence information
Length: 345 AA [This is the length of the unprocessed precursor] Molecular weight: 37167 Da [This is the MW of the unprocessed precursor] CRC64: BC809F2116B65871 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MARKWNLPFL LLPLVLGIPL VRGSNPLPLV VNTWPFKNAT EAAWWTLVSG GSALDAVEKG 

        70         80         90        100        110        120 
CAMCEKEQCG GTVGFGGSPD EVGETTLDAM IMDGTAMDVG AVGGLRRIKN AIGVARKVLE 

       130        140        150        160        170        180 
HTTHTLLVGD SATKFAVSMG FTSEDLSTNT SRALHSDWLS RNCQPNYWRN VIPDPSKYCG 

       190        200        210        220        230        240 
PYKPPDFLEQ NNRAHKEVDI HSHDTIGMVV IHKTGHTAAG TSTNGLKFKI PGRVGDSPIP 

       250        260        270        280        290        300 
GAGAYADDMA GAAAATGDGD TLLRFLPSYQ AVEYMRGGDD PARACQKVIS RIQKYYPKFF 

       310        320        330        340 
GAVICANVTG SYGAACNRLP TFTQFSFMVY NSLHNQAIEE KVDCM
P30919 in FASTA format

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