ID   LDHD_LACHE              Reviewed;         337 AA.
AC   P30901;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-NOV-2008, entry version 65.
DE   RecName: Full=D-lactate dehydrogenase;
DE            Short=D-LDH;
DE            EC=1.1.1.28;
DE   AltName: Full=D-specific D-2-hydroxyacid dehydrogenase;
OS   Lactobacillus helveticus.
OC   Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=1587;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-36.
RC   STRAIN=ATCC 15009 / DSM 20075 / IFO 15019 / JCM 1120 / Lh12;
RX   MEDLINE=93011111; PubMed=1396685;
RA   Kochhar S., Hottinger H., Chuard N., Taylor P.G., Atkinson T.,
RA   Scawen M.D., Nicholls D.J.;
RT   "Cloning and overexpression of Lactobacillus helveticus D-lactate
RT   dehydrogenase gene in Escherichia coli.";
RL   Eur. J. Biochem. 208:799-805(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CNRZ 32;
RX   MEDLINE=94338682; PubMed=7765104; DOI=10.1007/s002530050169;
RA   Bhowmik T.K., Steele J.L.;
RT   "Cloning, characterization and insertional inactivation of the
RT   Lactobacillus helveticus D(-) lactate dehydrogenase gene.";
RL   Appl. Microbiol. Biotechnol. 41:432-439(1994).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
RA   Bernard N., Delcour J., Alvarez A., Cortes A., Willis C.,
RA   Holbrook J.J.;
RL   Submitted (OCT-1995) to the PDB data bank.
CC   -!- CATALYTIC ACTIVITY: (R)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family.
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DR   EMBL; X66723; CAA47255.1; -; Genomic_DNA.
DR   EMBL; U07604; AAA20464.1; -; Genomic_DNA.
DR   PIR; S29296; S29296.
DR   PDB; 2DLD; X-ray; 2.70 A; A/B=1-337.
DR   PDBsum; 2DLD; -.
DR   LinkHub; P30901; -.
DR   GO; GO:0008720; F:D-lactate dehydrogenase activity; IEA:EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006139; D-isomer_2_OHA_DHase.
DR   InterPro; IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    337       D-lactate dehydrogenase.
FT                                /FTId=PRO_0000075954.
FT   ACT_SITE    236    236
FT   ACT_SITE    265    265
FT   ACT_SITE    297    297       Proton donor.
FT   STRAND        3      8
FT   TURN         11     13
FT   HELIX        14     23
FT   STRAND       28     30
FT   HELIX        40     43
FT   STRAND       48     52
FT   HELIX        60     67
FT   TURN         68     70
FT   STRAND       73     75
FT   STRAND       77     79
FT   HELIX        86     91
FT   HELIX       104    120
FT   HELIX       122    130
FT   HELIX       144    146
FT   STRAND      147    152
FT   HELIX       156    167
FT   STRAND      171    175
FT   HELIX       183    186
FT   HELIX       193    196
FT   TURN        197    199
FT   STRAND      201    205
FT   TURN        211    215
FT   HELIX       219    222
FT   STRAND      229    233
FT   HELIX       237    239
FT   HELIX       242    250
FT   STRAND      253    260
FT   HELIX       265    268
FT   HELIX       281    288
FT   STRAND      292    294
FT   HELIX       303    322
FT   STRAND      327    329
FT   TURN        333    335
SQ   SEQUENCE   337 AA;  37779 MW;  D0561DD82C03B3C4 CRC64;
     MTKVFAYAIR KDEEPFLNEW KEAHKDIDVD YTDKLLTPET AKLAKGADGV VVYQQLDYTA
     DTLQALADAG VTKMSLRNVG VDNIDMDKAK ELGFQITNVP VYSPNAIAEH AAIQAARVLR
     QDKRMDEKMA KRDLRWAPTI GREVRDQVVG VVGTGHIGQV FMRIMEGFGA KVIAYDIFKN
     PELEKKGYYV DSLDDLYKQA DVISLHVPDV PANVHMINDK SIAEMKDGVV IVNCSRGRLV
     DTDAVIRGLD SGKIFGFVMD TYEDEVGVFN KDWEGKEFPD KRLADLIDRP NVLVTPHTAF
     YTTHAVRNMV VKAFNNNLKL INGEKPDSPV ALNKNKF
//