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UniProtKB/Swiss-Prot entry P30901

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LDHD_LACHE
Primary accession number P30901
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1993
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 63)
Name and origin of the protein
Protein name D-lactate dehydrogenase
Synonyms D-LDH
EC 1.1.1.28
D-specific D-2-hydroxyacid dehydrogenase
Gene name None
From
Lactobacillus helveticus [TaxID: 1587] 
Taxonomy Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-36.
STRAIN=ATCC 15009 / DSM 20075 / IFO 15019 / JCM 1120 / Lh12;
PubMed=1396685 [NCBI, ExPASy, EBI, Israel, Japan]
Kochhar S., Hottinger H., Chuard N., Taylor P.G., Atkinson T., Scawen M.D., Nicholls D.J.;
"Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli.";
Eur. J. Biochem. 208:799-805(1992).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=CNRZ 32;
DOI=10.1007/s002530050169; PubMed=7765104 [NCBI, ExPASy, EBI, Israel, Japan]
Bhowmik T.K., Steele J.L.;
"Cloning, characterization and insertional inactivation of the Lactobacillus helveticus D(-) lactate dehydrogenase gene.";
Appl. Microbiol. Biotechnol. 41:432-439(1994).
[3]
 X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
Bernard N., Delcour J., Alvarez A., Cortes A., Willis C., Holbrook J.J.;
Submitted (OCT-1995) to the PDB data bank.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X66723; CAA47255.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U07604; AAA20464.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S29296; S29296.
3D structure databases
PDB
2DLD; X-ray; 2.70 A; A/B=1-337.[ExPASy / RCSB / EBI]
PDBsum 2DLD; -.
ModBase P30901.
Family and domain databases
InterPro IPR006139; D-isomer_2_OHA_DHase.
IPR006140; D-isomer_2_OHA_DHase_NAD-bd.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00389; 2-Hacid_dh; 1.
PF02826; 2-Hacid_dh_C; 1.
Pfam graphical view of domain structure.
PROSITE PS00065; D_2_HYDROXYACID_DH_1; 1.
PS00670; D_2_HYDROXYACID_DH_2; 1.
PS00671; D_2_HYDROXYACID_DH_3; 1.
BLOCKS P30901.
Other
LinkHub P30901; -.
ProtoNet P30901.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   337  336     D-lactate dehydrogenase. PRO_0000075954
ACT_SITE   236   236         
ACT_SITE   265   265         
ACT_SITE   297   297        Proton donor. 
STRAND   3     8  6      
TURN   11    13  3      
HELIX   14    23  10      
STRAND   28    30  3      
HELIX   40    43  4      
STRAND   48    52  5      
HELIX   60    67  8      
TURN   68    70  3      
STRAND   73    75  3      
STRAND   77    79  3      
HELIX   86    91  6      
HELIX   104   120  17      
HELIX   122   130  9      
HELIX   144   146  3      
STRAND   147   152  6      
HELIX   156   167  12      
STRAND   171   175  5      
HELIX   183   186  4      
HELIX   193   196  4      
TURN   197   199  3      
STRAND   201   205  5      
TURN   211   215  5      
HELIX   219   222  4      
STRAND   229   233  5      
HELIX   237   239  3      
HELIX   242   250  9      
STRAND   253   260  8      
HELIX   265   268  4      
HELIX   281   288  8      
STRAND   292   294  3      
HELIX   303   322  20      
STRAND   327   329  3      
TURN   333   335  3      
Sequence information
Length: 337 AA [This is the length of the unprocessed precursor] Molecular weight: 37779 Da [This is the MW of the unprocessed precursor] CRC64: D0561DD82C03B3C4 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTKVFAYAIR KDEEPFLNEW KEAHKDIDVD YTDKLLTPET AKLAKGADGV VVYQQLDYTA 

        70         80         90        100        110        120 
DTLQALADAG VTKMSLRNVG VDNIDMDKAK ELGFQITNVP VYSPNAIAEH AAIQAARVLR 

       130        140        150        160        170        180 
QDKRMDEKMA KRDLRWAPTI GREVRDQVVG VVGTGHIGQV FMRIMEGFGA KVIAYDIFKN 

       190        200        210        220        230        240 
PELEKKGYYV DSLDDLYKQA DVISLHVPDV PANVHMINDK SIAEMKDGVV IVNCSRGRLV 

       250        260        270        280        290        300 
DTDAVIRGLD SGKIFGFVMD TYEDEVGVFN KDWEGKEFPD KRLADLIDRP NVLVTPHTAF 

       310        320        330 
YTTHAVRNMV VKAFNNNLKL INGEKPDSPV ALNKNKF
P30901 in FASTA format

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