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UniProtKB/Swiss-Prot entry P30624


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LCF1_YEAST
Primary accession number P30624
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1993
Sequence was last modified on April 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 66)
Name and origin of the protein
Protein name Long-chain-fatty-acid--CoA ligase 1
Synonyms EC 6.2.1.3
Long-chain acyl-CoA synthetase 1
Fatty acid activator 1
Gene name
Name: FAA1
OrderedLocusNames: YOR317W
ORFNames: O6136
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 4-14.
STRAIN=ATCC 204508 / S288c;
DOI=10.1083/jcb.117.3.515; PubMed=1572893 [NCBI, ExPASy, EBI, Israel, Japan]
Duronio R.J., Knoll L.J., Gordon J.I.;
"Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation.";
J. Cell Biol. 117:515-529(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
DOI=10.1002/(SICI)1097-0061(199609)12:10B<1021::AID-YEA981>3.3.CO;2-Z; PubMed=8896266 [NCBI, ExPASy, EBI, Israel, Japan]
Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.;
"Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII.";
Yeast 12:1021-1031(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[4]
CHARACTERIZATION.
PubMed=8206942 [NCBI, ExPASy, EBI, Israel, Japan]
Knoll L.J., Johnson D.R., Gordon J.I.;
"Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p.";
J. Biol. Chem. 269:16348-16356(1994).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-622, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X66194; CAA46957.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X90565; CAA62172.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75225; CAA99637.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S23052; S23052.
RefSeq NP_014962.1; -.
3D structure databases
ModBase P30624.
Protein-protein interaction databases
DIP DIP:4654N; -.
IntAct P30624; -.
Organism-specific databases
CYGD YOR317w; -.
SGD S000005844; FAA1.
Yeast-GFP YOR317W.
Gene expression databases
ArrayExpress P30624; -.
GermOnline YOR317W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005811; Cellular component: lipid particle (inferred from direct assay from SGD).
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from direct assay from SGD).
GO:0004467; Molecular function: long-chain-fatty-acid-CoA ligase activity (inferred from electronic annotation from EC).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006631; Biological process: fatty acid metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0006869; Biological process: lipid transport (inferred from genetic interaction from SGD).
GO:0006499; Biological process: N-terminal protein myristoylation (inferred from genetic interaction from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.
Pfam PF00501; AMP-binding; 2.
Pfam graphical view of domain structure.
PRINTS PR00154; AMPBINDING.
PROSITE PS00455; AMP_BINDING; 1.
BLOCKS P30624.
ProtoNet P30624.
Proteomic databases
PeptideAtlas P30624; -.
Genome annotation databases
Ensembl YOR317W; Saccharomyces cerevisiae. [Contig view]
GeneID 854495; -.
GenomeReviews Y13140_GR; YOR317W.
KEGG sce:YOR317W; -.
NMPDR fig|4932.3.peg.6077; -.
Phylogenomic databases
HOGENOM P30624; -.
Other
LinkHub P30624; -.
NextBio 976828; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   700  700     Long-chain-fatty-acid--CoA ligase 1. PRO_0000193119
MOD_RES   622   622        Phosphoserine. 
Sequence information
Length: 700 AA [This is the length of the unprocessed precursor] Molecular weight: 77866 Da [This is the MW of the unprocessed precursor] CRC64: 56EE7E04D95BC5C9 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF QKNKNSNAMG 

        70         80         90        100        110        120 
WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN SFDQLTDIMH EIGRGLVKIG 

       130        140        150        160        170        180 
LKPNDDDKLH LYAATSHKWM KMFLGAQSQG IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD 

       190        200        210        220        230        240 
NSLLPSLIKP VQAAQDVKYI IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF 

       250        260        270        280        290        300 
DDILKLGKES CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK 

       310        320        330        340        350        360 
FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ GDLQEFKPTI 

       370        380        390        400        410        420 
MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM QRLHIPGGGA LGNLVFKKIR 

       430        440        450        460        470        480 
TATGGQLRYL LNGGSPISRD AQEFITNLIC PMLIGYGLTE TCASTTILDP ANFELGVAGD 

       490        500        510        520        530        540 
LTGCVTVKLV DVEELGYFAK NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG 

       550        560        570        580        590        600 
EWEANGHLKI IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV 

       610        620        630        640        650        660 
PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG LVGIELLAGI 

       670        680        690        700 
VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS 

P30624 in FASTA format

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View entry in raw text format (no links)
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