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UniProtKB/Swiss-Prot entry P30432


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FUR2_DROME
Primary accession number P30432
Secondary accession numbers A4V4M0 A4V4M2 Q24301 Q5BI10 Q8SZS2
Integrated into Swiss-Prot on April 1, 1993
Sequence was last modified on November 25, 2002 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 90)
Name and origin of the protein
Protein name Furin-like protease 2 [Precursor]
Synonyms Furin-2
EC 3.4.21.75
Gene name
Name: Fur2
ORFNames: CG18734
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D).
STRAIN=Iso-1, Oregon-R, and Tuebingen;
TISSUE=Embryo;
PubMed=1512259 [NCBI, ExPASy, EBI, Israel, Japan]
Roebroek A.J.M., Creemers J.W.M., Pauli I.G.L., Kurzik-Dumke U., Rentrop M., Gateff E.A.F., Leunissen J.A.M., van de Ven W.J.M.;
"Cloning and functional expression of Dfurin2, a subtilisin-like proprotein processing enzyme of Drosophila melanogaster with multiple repeats of a cysteine motif.";
J. Biol. Chem. 267:17208-17215(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Iso-1;
PubMed=7880443 [NCBI, ExPASy, EBI, Israel, Japan]
Roebroek A.J.M., Ayoubi T.A.Y., Creemers J.W.M., Pauli I.G.L., van de Ven W.J.M.;
"The Dfur2 gene of Drosophila melanogaster: genetic organization, expression during embryogenesis, and pro-protein processing activity of its translational product Dfurin2.";
DNA Cell Biol. 14:223-234(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[4]
GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
STRAIN=Berkeley;
TISSUE=Embryo;
Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 305-1679 (ISOFORM A).
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M94375; AAA28551.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L33831; AAA69860.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAF48598.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAF48599.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAN09399.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAN09400.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAN09401.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAN09402.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAS65387.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT021414; AAX33562.1; ALT_SEQ; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY070553; AAL48024.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A43434; A43434.
RefSeq NP_523368.2; -.
NP_727963.1; -.
NP_727964.1; -.
NP_727965.1; -.
NP_727966.1; -.
NP_727967.1; -.
NP_996486.1; -.
UniGene Dm.6383
3D structure databases
HSSP P23188; 1P8J. [HSSP ENTRY / PDB]
ModBase P30432.
Protein-protein interaction databases
IntAct P30432; -.
Protein family/group databases
MEROPS S08.049; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-002159-MON; -.
DMEL-XXX-02:DMEL-XXX-02-002161-MON; -.
DMEL-XXX-02:DMEL-XXX-02-002163-MON; -.
Organism-specific databases
FlyBase FBgn0004598; Fur2.
Gene expression databases
ArrayExpress P30432; -.
GermOnline CG18734; Drosophila melanogaster.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0004714; Molecular function: transmembrane receptor protein tyrosine kinase activity (inferred from electronic annotation from InterPro).
GO:0006468; Biological process: protein amino acid phosphorylation (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
GO:0007169; Biological process: transmembrane receptor protein tyrosine kinase signaling pathway (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR006210; EGF.
IPR006211; Furin-like.
IPR006212; Furin_repeat.
IPR000209; Pept_S8_S53.
IPR015500; Peptidase_S8_subtilisin-rel.
IPR002884; PrprotnconvertsP.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.200; Pept_S8_S53; 1.
PANTHER PTHR10795; SubtilSerProt; 1.
Pfam PF00757; Furin-like; 1.
PF01483; P_proprotein; 1.
PF00082; Peptidase_S8; 1.
Pfam graphical view of domain structure.
PRINTS PR00723; SUBTILISIN.
ProDom PD000717; PrprotnconvertsP; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00181; EGF; 1.
SM00261; FU; 10.
SMART graphical view of domain structure.
PROSITE PS00136; SUBTILASE_ASP; 1.
PS00137; SUBTILASE_HIS; 1.
PS00138; SUBTILASE_SER; 1.
BLOCKS P30432.
ProtoNet P30432.
Genome annotation databases
Ensembl CG18734; Drosophila melanogaster. [Contig view]
GeneID 32604; -.
KEGG dme:Dmel_CG18734; -.
Other
NextBio 779394; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Cleavage on pair of basic residues; Complete proteome; Glycoprotein; Hydrolase; Membrane; Protease; Repeat; Serine protease; Signal; Transmembrane; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
SIGNAL   1      ?        Potential. 
PROPEP   ?    318        Potential. PRO_0000027024
CHAIN   319   1679  1361     Furin-like protease 2. PRO_0000027025
TRANSMEM   1512   1532  21     Potential. 
TOPO_DOM   1533   1679  147     Cytoplasmic (Potential). 
REPEAT   961   1006  46     1. 
REPEAT   1007   1056  50     2. 
REPEAT   1057   1103  47     3. 
REPEAT   1104   1152  49     4. 
REPEAT   1153   1204  52     5. 
REPEAT   1205   1253  49     6. 
REPEAT   1254   1298  45     7. 
REPEAT   1299   1345  47     8. 
REPEAT   1346   1392  47     9. 
REPEAT   1393   1443  51     10. 
REGION   961   1443  483     10 X tandem repeats, Cys-rich. 
ACT_SITE   417    417        Charge relay system (By similarity). 
ACT_SITE   456    456        Charge relay system (By similarity). 
ACT_SITE   637    637        Charge relay system (By similarity). 
CARBOHYD   3      3        N-linked (GlcNAc...) (Potential). 
CARBOHYD   109    109        N-linked (GlcNAc...) (Potential). 
CARBOHYD   130    130        N-linked (GlcNAc...) (Potential). 
CARBOHYD   205    205        N-linked (GlcNAc...) (Potential). 
CARBOHYD   442    442        N-linked (GlcNAc...) (Potential). 
CARBOHYD   480    480        N-linked (GlcNAc...) (Potential). 
CARBOHYD   927    927        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1060   1060        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1181   1181        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1274   1274        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1277   1277        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1439   1439        N-linked (GlcNAc...) (Potential). 
DISULFID   473    629        By similarity. 
DISULFID   565    595        By similarity. 
DISULFID   720    748        By similarity. 
VAR_SEQ   386    386        L -> LVSK (in isoform A). VSP_009365
CONFLICT   152    153        Missing (in Ref. 1; AAA28551). 
CONFLICT   177    177        V -> F (in Ref. 1; AAA28551). 
CONFLICT   213    213        V -> VDQL (in Ref. 1; AAA28551). 
Sequence information
Length: 1679 AA [This is the length of the unprocessed precursor] Molecular weight: 183370 Da [This is the MW of the unprocessed precursor] CRC64: 3F9E749F0B021CF6 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSNTTRSSRV TIGRIGTTPQ ITDPWSSGLE KQRPSRCGGP KSLAEPTYRK IGRRKMMLHM 

        70         80         90        100        110        120 
RVHDPGTTVT QRAKETATAK LNRIYLCTFN RMAQSCIYFV LFLVILSPNT SCALRSSAGE 

       130        140        150        160        170        180 
TQNYVGILSN DSATTTYDVS SLHSSRRTNP PSSSSSSSSN VDVDYRNDRE LHKVDLVGLG 

       190        200        210        220        230        240 
GERAGQAETI SGGKYDYNYE NTHTNASAKD EIVERQSNSL DFDGVDMFGA FSIPEEAIYT 

       250        260        270        280        290        300 
NEFAVNIPAG KQMADVIATK HGFINRGQIG SLDNYYLFQH HHVSKRSLRS SRKHQGALKS 

       310        320        330        340        350        360 
ENEVKWMQQQ HEKVRRKRDG PYQDLPTYSP YNLLRQHGGY VVDPNPHLSF SPESISLASH 

       370        380        390        400        410        420 
SQRMEYRDVS SHFIFPDPLF KEQWYLNGGA KDGLDMNVGP AWQKGYTGKG VVVSILDDGI 

       430        440        450        460        470        480 
QTNHPDLAQN YDPEASFDIN GNDSDPTPQD NGDNKHGTRC AGEVAAVAFN NFCGVGVAYN 

       490        500        510        520        530        540 
ASIGGVRMLD GKVNDVVEAQ ALSLNPSHID IYSASWGPED DGSTVDGPGP LARRAFIYGV 

       550        560        570        580        590        600 
TSGRQGKGSI FVWASGNGGR YTDSCNCDGY TNSIFTLSIS SATQAGFKPW YLEECSSTLA 

       610        620        630        640        650        660 
TTYSSGTPGH DKSVATVDMD GSLRPDHICT VEHTGTSASA PLAAGICALA LEANPELTWR 

       670        680        690        700        710        720 
DMQYLVVYTS RPAPLEKENG WTLNGVKRKY SHKFGYGLMD AGAMVSLAEQ WTSVPPQHIC 

       730        740        750        760        770        780 
KSRENNEDRK IDGAYGSTLS THMDVNGCAG TINEVRYLEH VQCRITLRFF PRGNLRILLT 

       790        800        810        820        830        840 
SPMGTTSTLL FERPRDIVKS NFDDWPFLSV HFWGEKAEGR WTLQVINGGR RRVNQPGILS 

       850        860        870        880        890        900 
KWQLIFYGTS TQPMRLKSEL LNSSPQLRSP SSSNPFLFPS ASNIGQPANE GGNFNTDSFA 

       910        920        930        940        950        960 
SYLNYQNIFS SAGSDPEPAT ATLDGQNVTA AIAGGSSAES LGFTASAAQL VAAPETRDGD 

       970        980        990       1000       1010       1020 
KKILHSCDAE CDSSGCYGRG PTQCVACSHY RLDNTCVSRC PPRSFPNQVG ICWPCHDTCE 

      1030       1040       1050       1060       1070       1080 
TCAGAGPDSC LTCAPAHLHV IDLAVCLQFC PDGYFENSRN RTCVPCEPNC ASCQDHPEYC 

      1090       1100       1110       1120       1130       1140 
TSCDHHLVMH EHKCYSACPL DTYETEDNKC AFCHSTCATC NGPTDQDCIT CRSSRYAWQN 

      1150       1160       1170       1180       1190       1200 
KCLISCPDGF YADKKRLECM PCQEGCKTCT SNGVCSECLQ NWTLNKRDKC IVSGSEGCSE 

      1210       1220       1230       1240       1250       1260 
SEFYSQVEGQ CRPCHASCGS CNGPADTSCT SCPPNRLLEQ SRCVSGCREG FFVEAGSLCS 

      1270       1280       1290       1300       1310       1320 
PCLHTCSQCV SRTNCSNCSK GLELQNGECR TTCADGYYSD RGICAKCYLS CHTCSGPRRN 

      1330       1340       1350       1360       1370       1380 
QCVQCPAGWQ LAAGECHPEC PEGFYKSDFG CQKCHHYCKT CNDAGPLACT SCPPHSMLDG 

      1390       1400       1410       1420       1430       1440 
GLCMECLSSQ YYDTTSATCK TCHDSCRSCF GPGQFSCKGC VPPLHLDQLN SQCVSCCQNQ 

      1450       1460       1470       1480       1490       1500 
TLAEKTSSAA CCNCDGETGE CKATSTGGKR RTVVGSGSAY KSSESKHGSF ENDGNAREFV 

      1510       1520       1530       1540       1550       1560 
LRLDSPLTAI TAIAVAICLL IITIFSIIFA VLQRNSNHVS RNSVRYRKIA NTSSGRRKNL 

      1570       1580       1590       1600       1610       1620 
SAKPTSDARF IFNIGEDDDT DGDNSDDELD GNVGTDINNR IVYDRKGNDH GHEFYIESTN 

      1630       1640       1650       1660       1670 
DIDAIEFHCN GAGAQKAETQ LQRCNANGDD DDILHYDRHT NAERKNHPSS TTSRTNIRS 

P30432 in FASTA format

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