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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(92)90460-7; PubMed=1312269 [NCBI, ExPASy, EBI, Israel, Japan]
Blok J.,
McWilliam S.M.,
Butler H.C.,
Gibbs A.J.,
Weiller G.,
Herring B.L.,
Hemsley A.C.,
Aaskov J.G.,
Yoksan S.,
Bhamarapravati N.;
"Comparison of a dengue-2 virus and its candidate vaccine derivative: sequence relationships with the flaviviruses and other viruses.";
Virology 187:573-590(1992).
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[2]
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PROTEOLYTIC PROCESSING OF POLYPROTEIN.
DOI=10.1128/JVI.78.5.2367-2381.2004; PubMed=14963133 [NCBI, ExPASy, EBI, Israel, Japan]
Keelapang P.,
Sriburi R.,
Supasa S.,
Panyadee N.,
Songjaeng A.,
Jairungsri A.,
Puttikhunt C.,
Kasinrerk W.,
Malasit P.,
Sittisombut N.;
"Alterations of pr-M cleavage and virus export in pr-M junction chimeric dengue viruses.";
J. Virol. 78:2367-2381(2004).
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[3]
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FUNCTION OF NON-STRUCTURAL PROTEIN 4B.
DOI=10.1128/JVI.79.13.8004-8013.2005; PubMed=15956546 [NCBI, ExPASy, EBI, Israel, Japan]
Munoz-Jordan J.L.,
Laurent-Rolle M.,
Ashour J.,
Martinez-Sobrido L.,
Ashok M.,
Lipkin W.I.,
Garcia-Sastre A.;
"Inhibition of alpha/beta interferon signaling by the NS4B protein of flaviviruses.";
J. Virol. 79:8004-8013(2005).
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[4]
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FUNCTION OF NS1.
DOI=10.1086/500949; PubMed=16544248 [NCBI, ExPASy, EBI, Israel, Japan]
Avirutnan P.,
Punyadee N.,
Noisakran S.,
Komoltri C.,
Thiemmeca S.,
Auethavornanan K.,
Jairungsri A.,
Kanlaya R.,
Tangthawornchaikul N.,
Puttikhunt C.,
Pattanakitsakul S.N.,
Yenchitsomanus P.T.,
Mongkolsapaya J.,
Kasinrerk W.,
Sittisombut N.,
Husmann M.,
Blettner M.,
Vasanawathana S.,
Bhakdi S.,
Malasit P.;
"Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement.";
J. Infect. Dis. 193:1078-1088(2006).
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[5]
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POLYPROTEIN CLEAVAGE.
DOI=10.1042/BJ20061136; PubMed=17067286 [NCBI, ExPASy, EBI, Israel, Japan]
Shiryaev S.A.,
Kozlov I.A.,
Ratnikov B.I.,
Smith J.W.,
Lebl M.,
Strongin A.Y.;
"Cleavage preference distinguishes the two-component NS2B-NS3 serine proteinases of Dengue and West Nile viruses.";
Biochem. J. 401:743-752(2007).
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[6]
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CHARACTERIZATION OF NS1.
DOI=10.1016/j.jviromet.2007.01.008; PubMed=17331594 [NCBI, ExPASy, EBI, Israel, Japan]
Noisakran S.,
Dechtawewat T.,
Rinkaewkan P.,
Puttikhunt C.,
Kanjanahaluethai A.,
Kasinrerk W.,
Sittisombut N.,
Malasit P.;
"Characterization of dengue virus NS1 stably expressed in 293T cell lines.";
J. Virol. Methods 142:67-80(2007).
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- FUNCTION: Protein C packages viral RNA to form a viral nucleocapsid, and promotes virion budding (By similarity).
- FUNCTION: prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).
- FUNCTION: Envelope protein E binds cell surface receptor and is involved in membrane fusion between virion and target cell. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).
- FUNCTION: Non-structural protein 1 is slowly secreted from mammalian cells, but not from mosquito cells. Secreted form elicits protective immune response and plays an essential role in RNA replication (By similarity). Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome.
- FUNCTION: Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity).
- FUNCTION: Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).
- FUNCTION: Non-structural protein 4A plays a role in RNA replication. Enhances inhibition of cell antiviral response by non-structural protein 4B (By similarity).
- FUNCTION: Non-structural protein 4B prevent the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways (By similarity).
- FUNCTION: RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and assure the capping of genomes in the cytoplasm. May be involved in methylation of 5'RNA cap structure (By similarity).
- CATALYTIC ACTIVITY: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
- SUBUNIT: prM and envelope protein E form heterodimers in the endoplasmic reticulum and Golgi. Envelope protein E forms homodimers. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A. NS3 and NS2B form an heterodimer. NS3 interacts with unphosphorylated NS5 (By similarity).
- SUBCELLULAR LOCATION: Protein C: Virion (By similarity).
- SUBCELLULAR LOCATION: Peptide pr: Secreted (By similarity).
- SUBCELLULAR LOCATION: Small envelope protein M: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Envelope protein E: Virion membrane; Single-pass type I membrane protein (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 1: Secreted. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A-alpha: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 2A: Endoplasmic reticulum membrane (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS2B: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Serine protease subunit NS3: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4A: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
- SUBCELLULAR LOCATION: Non-structural protein 4B: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=The C-terminal transmembrane domain of non-structural protein 4B is presumably reoriented after cleavage on the lumenal side (By similarity).
- SUBCELLULAR LOCATION: RNA-directed RNA polymerase NS5: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus (By similarity).
- DOMAIN: Transmembrane domains of the small envelope protein M and envelope protein E contains an endoplasmic reticulum retention signals (By similarity).
- PTM: Specific enzymatic cleavages in vivo yield mature proteins. The nascent protein C contains a C-terminal hydrophobic domain that act as a signal sequence for translocation of prM into the lumen of the ER. Mature protein C is cleaved at a site upstream of this hydrophobic domain by NS3. prM is cleaved in post-Golgi vesicles by a host furin, releasing the mature small envelope protein M, and peptide pr. Non-structural protein 2A-alpha, a C-terminally truncated form of non-structural protein 2A, results from partial cleavage by NS3 (By similarity).
- PTM: RNA-directed RNA polymerase NS5 is phosphorylated on serines residues. This phosphorylation may trigger NS5 nuclear localization (By similarity).
- PTM: Envelope protein E and non-structural protein 1 are N-glycosylated (By similarity).
- MISCELLANEOUS: The virion is assembled in the endoplasmic reticulum lumen, transported by vesicles to the Golgi, then transported again to the cell membrane where it is released outside the cell.
- SIMILARITY: Contains 1 helicase ATP-binding domain.
- SIMILARITY: Contains 1 helicase C-terminal domain.
- SIMILARITY: Contains 1 peptidase S7 domain [view classification].
- SIMILARITY: Contains 1 RdRp catalytic domain.
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Distributed under the Creative Commons Attribution-NoDerivs License.
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