ID   NANH_CLOSE              Reviewed;        1014 AA.
AC   P29767;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-NOV-2008, entry version 56.
DE   RecName: Full=Sialidase;
DE            EC=3.2.1.18;
DE   AltName: Full=Neuraminidase;
DE   Flags: Precursor;
OS   Clostridium septicum.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1504;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NC 0054714;
RX   MEDLINE=91238693; PubMed=2034213; DOI=10.1007/BF00273603;
RA   Rothe B., Rothe B., Roggentin P., Schauer R.;
RT   "The sialidase gene from Clostridium septicum: cloning, sequencing,
RT   expression in Escherichia coli and identification of conserved
RT   sequences in sialidases and other proteins.";
RL   Mol. Gen. Genet. 226:190-197(1991).
CC   -!- FUNCTION: Sialidases have been suggested to be pathogenic factors
CC       in microbial infections.
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-,
CC       alpha-(2->8)- glycosidic linkages of terminal sialic acid residues
CC       in oligosaccharides, glycoproteins, glycolipids, colominic acid
CC       and synthetic substrates.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 33 family.
CC   -!- SIMILARITY: Contains 4 BNR repeats.
CC   -!- SIMILARITY: Contains 1 F5/8 type C domain.
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DR   EMBL; X63266; CAA44916.1; -; Genomic_DNA.
DR   PIR; S15994; NMCLSS.
DR   HSSP; Q27701; 1SLL.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0004308; F:exo-alpha-sialidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   InterPro; IPR002860; BNR.
DR   InterPro; IPR000421; Coagulation_factor_5/8-type_C.
DR   InterPro; IPR013320; ConA_like_subgrp.
DR   InterPro; IPR011490; FIVAR_sugar_bd.
DR   InterPro; IPR004124; Glyco_hydro_33_N.
DR   Gene3D; G3DSA:2.60.120.200; ConA_like_subgrp; 1.
DR   Pfam; PF02012; BNR; 4.
DR   Pfam; PF00754; F5_F8_type_C; 1.
DR   Pfam; PF07554; FIVAR; 1.
DR   Pfam; PF02973; Sialidase; 1.
DR   PROSITE; PS50022; FA58C_3; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Repeat; Secreted; Signal.
FT   SIGNAL        1     26       Potential.
FT   CHAIN        27   1014       Sialidase.
FT                                /FTId=PRO_0000012031.
FT   DOMAIN       39    186       F5/8 type C.
FT   REPEAT      431    442       BNR 1.
FT   REPEAT      563    574       BNR 2.
FT   REPEAT      627    638       BNR 3.
FT   REPEAT      700    711       BNR 4.
FT   ACT_SITE    421    421       Proton acceptor (By similarity).
FT   ACT_SITE    912    912       Nucleophile (By similarity).
FT   BINDING     396    396       Substrate (By similarity).
FT   BINDING     688    688       Substrate (By similarity).
FT   BINDING     873    873       Substrate (Potential).
SQ   SEQUENCE   1014 AA;  110653 MW;  C4F49233473A2FAD CRC64;
     MNKKKIMSIL VSAFLITNLS SNIIFADIKE NVYINQYSEG NRSQPIAEKL VPRSEIQASA
     TSALTGEGPE KAIDGNTSTL WHTPWAGVDI QINPQSLTLK LGKTRNISSI CVTPRQEGTN
     GMITDYKIYS GDDVIAEGKW KSDSSDKYVV FDNPISTDNI RIEAISTVGD ENNKHASIAE
     VEVYELADTP VKLAESNNKV INNGNGGNYE GDISEISLLE EGTAIIRFTN NGSSLFSISN
     NERTNEHFHV YINGGAIGYE LRKQSGNLAT GSVNKALNAG INTIAFKAEK GKGYSIYLNG
     EKILTSSSIT ANFLSTLEGL NTLSLGKTDR PSGSNEYNFT GEIDFFELYS KPLADRYLKE
     RTGETTSKDL PFPEGAVKTE PVDIFTPGEL GSNNFRIPAL YTTKDGTVLA SIDVRKGGGH
     DAPNNIDTGI KRSTDGGVTW DEGKIILDYP GASSAIDTSL LQDDETGRIF LIVTHFAEGY
     GFGNSKTGSG YVEIEGKRYL KLLGANDTIY TVREGVVYDS NGEATNYTVD NNNELYENGN
     RIGNVLLSNS PLKVMGTSFL SLIYSDDDGQ TWSDPIDLNK EVKTDWMRFL GTGPGKGHQI
     KTGRYAGRLL FPVYLTNASG FQSSAVIYSD DNGATWNIGE TATDGRLMDN GDRASAETIT
     TNTSGGVGQL TECQVVEMPN GQLKMFMRNT GGNSGRVRIA TSFDGGATWE DDVVRDENIK
     EPYCQLSVIN YSQKIDGKDA IIFAIPDANY PNRVNGTVRV GLITENGSYE NGEPRYDIEW
     RYNKVVAPGT YGYSCLSEMP NGEIGLFYEG RGSRQMSFTR MNIDYLKADL LQDVPAANIK
     SYTTNSENNI YDPGDKISLN VTFDQTVSLI GDRTITADIG GKEVLLTLAN SKGGSEYTFE
     GTVPADISNG NYTITIKGKS GLKIVNVVNK VTDITEDRNT GLNVQVGEEV QSVDKTLLQD
     LVDSTSNLIK EDYTEESWIL YEKALEVANK FLVNEIAVQE EVDAAKPTLE NAYK
//