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[1]
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X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).
DOI=10.1016/0022-2836(92)90662-4; PubMed=1738156 [NCBI, ExPASy, EBI, Israel, Japan]
Betzel C.,
Klupsch S.,
Papendorf G.,
Hastrup S.,
Branner S.,
Wilson K.S.;
"Crystal structure of the alkaline proteinase Savinase from Bacillus lentus at 1.4-A resolution.";
J. Mol. Biol. 223:427-445(1992).
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[2]
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STRUCTURE BY NMR.
PubMed=8654411 [NCBI, ExPASy, EBI, Israel, Japan]
Remerowski M.L.,
Pepermans H.A.M.,
Hilbers C.W.,
van de Ven F.J.M.;
"Backbone dynamics of the 269-residue protease Savinase determined from 15N-NMR relaxation measurements.";
Eur. J. Biochem. 235:629-640(1996).
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[3]
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X-RAY CRYSTALLOGRAPHY (0.78 ANGSTROMS).
DOI=10.1021/bi9813983; PubMed=9753430 [NCBI, ExPASy, EBI, Israel, Japan]
Kuhn P.,
Knapp M.,
Soltis S.M.,
Ganshaw G.,
Thoene M.,
Bott R.;
"The 0.78-A structure of a serine protease: Bacillus lentus subtilisin.";
Biochemistry 37:13446-13452(1998).
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- FUNCTION: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
- CATALYTIC ACTIVITY: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
- COFACTOR: Binds 2 calcium ions per subunit.
- SUBCELLULAR LOCATION: Secreted.
- BIOTECHNOLOGY: Used as a detergent protease. Sold under the name Savinase by Novozymes.
- MISCELLANEOUS: Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.
- SIMILARITY: Belongs to the peptidase S8 family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 269 AA [This is the length of the unprocessed precursor] |
Molecular weight: 26698 Da [This is the MW of the unprocessed precursor] |
CRC64: 4D89F8778999BF8D [This is a checksum on the sequence] |
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10 20 30 40 50 60
AQSVPWGISR VQAPAAHNRG LTGSGVKVAV LDTGISTHPD LNIRGGASFV PGEPSTQDGN
70 80 90 100 110 120
GHGTHVAGTI AALNNSIGVL GVAPSAELYA VKVLGASGSG SVSSIAQGLE WAGNNGMHVA
130 140 150 160 170 180
NLSLGSPSPS ATLEQAVNSA TSRGVLVVAA SGNSGAGSIS YPARYANAMA VGATDQNNNR
190 200 210 220 230 240
ASFSQYGAGL DIVAPGVNVQ STYPGSTYAS LNGTSMATPH VAGAAALVKQ KNPSWSNVQI
250 260
RNHLKNTATS LGSTNLYGSG LVNAEAATR
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P29600 in FASTA format |
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