[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 40-46. STRAIN=Aojuku; TISSUE=Midgut; DOI=10.1016/0167-4781(91)90229-F; PubMed=1756175 [NCBI, ExPASy, EBI, Israel, Japan] Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.; "Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."; Biochim. Biophys. Acta 1129:135-138(1991).
[2]	SEQUENCE REVISION TO N-TERMINUS. Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.; Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=Aojuku; TISSUE=Midgut; DOI=10.1007/s00438-003-0880-9; PubMed=14508679 [NCBI, ExPASy, EBI, Israel, Japan] Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.; "Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."; Mol. Genet. Genomics 270:114-120(2003).
[4]	SEQUENCE REVISION TO N-TERMINUS. Itoh M., Kanamori Y., Nishida S.; Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: A phosphate monoester + H₂O = an alcohol + phosphate.
COFACTOR: Binds 1 magnesium ion (By similarity).
COFACTOR: Binds 2 zinc ions (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor (By similarity).
TISSUE SPECIFICITY: Midgut.
SIMILARITY: Belongs to the alkaline phosphatase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D90454; BAA14420.3; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB055428; BAB62745.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S19607; S19607.

NP_001037536.2; -.

3D structure databases

HSSP

P05187; 1EW2. [HSSP ENTRY / PDB]

ModBase

P29523.

Ontologies

GO:0031225;	Cellular component: anchored to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004035;	Molecular function: alkaline phosphatase activity (inferred from electronic annotation from EC).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR017849; Alkaline_Pase-like_a/b/a.
IPR001952; Alkaline_phosphatase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.720.10; Alk_phosphtse; 1.

Pfam

PF00245; Alk_phosphatase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00113; ALKPHPHTASE.

SMART

SM00098; alkPPc; 1.
SMART graphical view of domain structure.

PROSITE

PS00123; ALKALINE_PHOSPHATASE; 1.

Genome annotation databases

GeneID

693074; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Phosphoprotein; Signal; Zinc.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 39`	`39`
`CHAIN`	`40 516`	`477`	`Membrane-bound alkaline phosphatase.`	`PRO_0000024047`
`PROPEP`	`517 550`	`34`	`Removed in mature form (Potential).`	`PRO_0000024048`
`COMPBIAS`	`10 74`	`65`	`Ala-rich.`
`ACT_SITE`	`133 133`		`Phosphoserine intermediate (By similarity).`
`METAL`	`83 83`		`Magnesium (By similarity).`
`METAL`	`83 83`		`Zinc 2 (By similarity).`
`METAL`	`196 196`		`Magnesium (By similarity).`
`METAL`	`198 198`		`Magnesium (By similarity).`
`METAL`	`356 356`		`Magnesium (By similarity).`
`METAL`	`361 361`		`Zinc 1 (By similarity).`
`METAL`	`365 365`		`Zinc 1 (By similarity).`
`METAL`	`402 402`		`Zinc 2 (By similarity).`
`METAL`	`403 403`		`Zinc 2 (By similarity).`
`METAL`	`479 479`		`Zinc 1 (By similarity).`
`LIPID`	`516 516`		`GPI-anchor amidated glycine (Potential).`
`CONFLICT`	`59 59`		`T -> R (in Ref. 1 and 2; BAA14420).`
`CONFLICT`	`439 439`		`A -> T (in Ref. 1 and 2; BAA14420).`

Sequence information

Length: 550 AA [This is the length of the unprocessed precursor]

Molecular weight: 60179 Da [This is the MW of the unprocessed precursor]

CRC64: C3F4C95A7F69CD57 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV REAQEAIETR 

        70         80         90        100        110        120 
EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR RGQTGEEASL HFEQFPTLGL 

       130        140        150        160        170        180 
AKTYCVNAQV PDSSCTATAY LCGVKANQGT PGVTAAVPRH DCEASTDVTK RVQSIAEWAL 

       190        200        210        220        230        240 
ADGRDVGIVT TTRITHASPA GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG 

       250        260        270        280        290        300 
NKFKVIFGGG RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL 

       310        320        330        340        350        360 
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG FFLFVEGGRI 

       370        380        390        400        410        420 
DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT ADHTHVMSFN GYSPRGTDVL 

       430        440        450        460        470        480 
GTVRSLDSNR MPFMVLSYAN GPGARIQQNG VRPDVTTDAN FGALRWRTHT DVPLDSETHG 

       490        500        510        520        530        540 
GDDVTVFAWG VHHWMFSGLY EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA 

       550 
FITLRHQCFL

P29523 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools