ID   DHOM_PSEAE              Reviewed;         434 AA.
AC   P29365;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   25-NOV-2008, entry version 64.
DE   RecName: Full=Homoserine dehydrogenase;
DE            Short=HDH;
DE            EC=1.1.1.3;
GN   Name=hom; OrderedLocusNames=PA3736;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=93086420; PubMed=1333566;
RA   Clepet C., Borne F., Krishnapillai V., Baird C., Patte J.-C., Cami B.;
RT   "Isolation, organization and expression of the Pseudomonas aeruginosa
RT   threonine genes.";
RL   Mol. Microbiol. 6:3109-3119(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY: L-homoserine + NAD(P)(+) = L-aspartate 4-
CC       semialdehyde + NAD(P)H.
CC   -!- ENZYME REGULATION: Feedback inhibition by threonine.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de
CC       novo pathway; L-homoserine from L-aspartate: step 3/3.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-
CC       threonine from L-aspartate: step 3/5.
CC   -!- SIMILARITY: Belongs to the homoserine dehydrogenase family.
CC   -!- SIMILARITY: Contains 1 ACT domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA46167.1; Type=Frameshift; Positions=Several;
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DR   EMBL; X65033; CAA46167.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AE004091; AAG07123.1; -; Genomic_DNA.
DR   PIR; H83179; H83179.
DR   PIR; S27979; DEPSHA.
DR   RefSeq; NP_252425.1; -.
DR   GeneID; 880337; -.
DR   GenomeReviews; AE004091_GR; PA3736.
DR   KEGG; pae:PA3736; -.
DR   NMPDR; fig|208964.1.peg.3735; -.
DR   PseudoCAP; PA3736; -.
DR   HOGENOM; P29365; -.
DR   BioCyc; PAER208964:PA3736-MON; -.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002912; ACT_bd.
DR   InterPro; IPR005106; Asp/hSer_DHase_NAD-bd.
DR   InterPro; IPR016204; hSer_DHase.
DR   InterPro; IPR001342; hSer_DHase_cat.
DR   Pfam; PF01842; ACT; 1.
DR   Pfam; PF00742; Homoserine_dh; 1.
DR   Pfam; PF03447; NAD_binding_3; 1.
DR   PIRSF; PIRSF000098; Homoser_dehydrog; 1.
DR   PROSITE; PS01042; HOMOSER_DHGENASE; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Branched-chain amino acid biosynthesis;
KW   Complete proteome; Isoleucine biosynthesis; Methionine biosynthesis;
KW   NADP; Oxidoreductase; Threonine biosynthesis.
FT   CHAIN         1    434       Homoserine dehydrogenase.
FT                                /FTId=PRO_0000066703.
FT   DOMAIN      352    427       ACT.
FT   NP_BIND       9     16       NADP (By similarity).
FT   ACT_SITE    203    203       Proton donor (Potential).
FT   BINDING     103    103       NADP (By similarity).
FT   BINDING     188    188       Substrate (By similarity).
FT   CONFLICT     81     85       YTLAH -> PPWPI (in Ref. 1; CAA46167).
FT   CONFLICT    187    190       AEAD -> GRGRS (in Ref. 1; CAA46167).
FT   CONFLICT    202    202       H -> T (in Ref. 1; CAA46167).
FT   CONFLICT    210    214       IAFGI -> MLRH (in Ref. 1; CAA46167).
FT   CONFLICT    276    277       NG -> TA (in Ref. 1; CAA46167).
FT   CONFLICT    402    402       R -> H (in Ref. 1; CAA46167).
SQ   SEQUENCE   434 AA;  46224 MW;  68770627B8AD9746 CRC64;
     MKPVKVGICG LGTVGGGTFN VLERNAEEIA RRAGRGIEVA QIAARRPNPK CDTGATPITA
     DIFDVACNPE IDVVVELIGG YTLAHELVLK AIENGKHVVT ANKALIAVHG NEIFAKAREK
     GVIVAFEAAV AGGIPVIKAI REGLSANRIN WLAGIINGTG NFILSEMREK GRTFPDVLAE
     AQALGYAEAD PTFDVEGIDA AHKLTILASI AFGIPLQFDK AYTEGISKLT SADVNYADAL
     GYRIKHLGVA RRTESGFELR VHPTLIPSDR LIANVNGVMN AVMVNGDAVG STLYYGAGAG
     MEPTASSVVA DLVDVVRAMT SDPENRVPHL AFQPDALSDH PILPIEACES AYYLRIQAKD
     HPGVLAQVAT ILSERGINIE SIMQKEAEEQ DGLVPMILVT HRVIEQRIND AIAALEALEG
     VSGPVVRIRV EQLN
//