ID   3HIDH_RAT               Reviewed;         335 AA.
AC   P29266; A1L107;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2001, sequence version 3.
DT   25-NOV-2008, entry version 79.
DE   RecName: Full=3-hydroxyisobutyrate dehydrogenase, mitochondrial;
DE            Short=HIBADH;
DE            EC=1.1.1.31;
DE   Flags: Precursor;
GN   Name=Hibadh;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Liver;
RX   MEDLINE=89174651; PubMed=2647728;
RA   Rougraff P.M., Zhang B., Kuntz M.J., Harris R.A., Crabb D.W.;
RT   "Cloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate
RT   dehydrogenase. Evidence for its evolutionary relationship to other
RT   pyridine nucleotide-dependent dehydrogenases.";
RL   J. Biol. Chem. 264:5899-5903(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 60-75; 297-310 AND 321-330, AND MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Spinal cord;
RA   Lubec G., Afjehi-Sadat L.;
RL   Submitted (NOV-2006) to UniProtKB.
RN   [4]
RP   MUTAGENESIS.
RX   MEDLINE=96335606; PubMed=8766712; DOI=10.1016/0014-5793(96)00597-2;
RA   Hawes J.W., Harper E.T., Crabb D.W., Harris R.A.;
RT   "Structural and mechanistic similarities of 6-phosphogluconate and 3-
RT   hydroxyisobutyrate dehydrogenases reveal a new enzyme family, the 3-
RT   hydroxyacid dehydrogenases.";
RL   FEBS Lett. 389:263-267(1996).
CC   -!- CATALYTIC ACTIVITY: 3-hydroxy-2-methylpropanoate + NAD(+) = 2-
CC       methyl-3-oxopropanoate + NADH.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- TISSUE SPECIFICITY: Higher level in kidney, liver, and heart than
CC       in muscle.
CC   -!- SIMILARITY: Belongs to the 3-hydroxyisobutyrate dehydrogenase
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA50312.1; Type=Frameshift; Positions=10;
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DR   EMBL; J04628; AAA50312.1; ALT_FRAME; mRNA.
DR   EMBL; BC127442; AAI27443.1; -; mRNA.
DR   PIR; A32867; A32867.
DR   RefSeq; NP_071579.1; -.
DR   UniGene; Rn.73; -.
DR   SMR; P29266; 40-334.
DR   Ensembl; ENSRNOG00000008063; Rattus norvegicus.
DR   GeneID; 63938; -.
DR   KEGG; rno:63938; -.
DR   RGD; 708399; Hibadh.
DR   HOVERGEN; P29266; -.
DR   NextBio; 612544; -.
DR   ArrayExpress; P29266; -.
DR   GermOnline; ENSRNOG00000008063; Rattus norvegicus.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-KW.
DR   GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0004616; F:phosphogluconate dehydrogenase (decarboxyla...; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
DR   GO; GO:0006573; P:valine metabolic process; IEA:InterPro.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DHase-rel_CS.
DR   InterPro; IPR015815; 3hydroxyacid_DHase/Rdtase.
DR   InterPro; IPR006183; 6-phosphogluconate_DHase.
DR   InterPro; IPR006115; 6PGDH_NAD-bd.
DR   InterPro; IPR013328; DHase_multihelical.
DR   InterPro; IPR011548; IsoBut3OH_DH.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR22981; 3hydroxy_acid_DH; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PRINTS; PR00076; 6PGDHDRGNASE.
DR   TIGRFAMs; TIGR01692; HIBADH; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase;
KW   Transit peptide.
FT   TRANSIT       1     35       Mitochondrion (By similarity).
FT   CHAIN        36    335       3-hydroxyisobutyrate dehydrogenase,
FT                                mitochondrial.
FT                                /FTId=PRO_0000007160.
FT   NP_BIND      39     68       NAD (By similarity).
FT   NP_BIND     102    103       NAD (By similarity).
FT   ACT_SITE    208    208       Probable.
FT   BINDING     107    107       NAD (By similarity).
FT   BINDING     133    133       NAD; via amide nitrogen (By similarity).
FT   BINDING     283    283       NAD (By similarity).
FT   MUTAGEN      68     68       D->R: Decrease of activity with NAD,
FT                                increase of activity with NADP.
FT   MUTAGEN     208    208       K->A,H,N,R: Complete loss of activity.
FT   MUTAGEN     212    212       N->Q: Decrease in activity.
SQ   SEQUENCE   335 AA;  35303 MW;  D266A7838500295A CRC64;
     MAASLGFRGA ASGLRYWSGR RRPVGSLAAV CSRSMASKTP VGFIGLGNMG NPMAKNLIKH
     GYPLILYDVF PDVCKEFKEA GEQVASSPAD VAEKADRIIT MLPSSMNSIE VYSGANGILK
     KVKKGSLLID SSTIDPSVSK ELAKEVEKMG AVFMDAPVSG GVGAARSGNL TFMVGGVENE
     FAAAQELLGC MGSNVLYCGA VGSGQSAKIC NNMLLAISMI GTAEAMNLGI RSGLDPKLLA
     KILNMSSGRC WSSDTYNPVP GVMDGVPSSN NYQGGFGTTL MAKDLGLAQD SATSTKTPIL
     LGSVAHQIYR MMCSKGYSKK DFSSVFQYLR EEETF
//