[1]	PROTEIN SEQUENCE. TISSUE=Thymus; DOI=10.1093/nar/18.1.17; PubMed=2308822 [NCBI, ExPASy, EBI, Israel, Japan] Rydberg B., Hall J., Karran P.; "Active site amino acid sequence of the bovine O6-methylguanine-DNA methyltransferase."; Nucleic Acids Res. 18:17-21(1990).

Comments

FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
COFACTOR: Binds 1 zinc ion (By similarity).
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Belongs to the MGMT family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

ModBase

P29177.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0003908;	Molecular function: methylated-DNA-[protein]-cysteine S-methyltransferase activity (inferred from electronic annotation from EC).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006281;	Biological process: DNA repair (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001497; MethylDNA_cys_MeTrfase_AS.
Graphical view of domain structure.

PROSITE

PS00374; MGMT; PARTIAL.

Phylogenomic databases

HOVERGEN

P29177; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Metal-binding; Methyltransferase; Nucleus; Transferase; Zinc.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >9`	`>9`	`Methylated-DNA--protein-cysteine methyltransferase.`	`PRO_0000139356`
`ACT_SITE`	`9 9`		`Nucleophile; methyl group acceptor (By similarity).`
`NON_TER`	`1 1`
`NON_TER`	`9 9`

Sequence information

Length: 9 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 967 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 325171A720476047 [This is a checksum on the sequence]

NPIPILTPC

P29177 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools