ID   BDH_RAT                 Reviewed;         343 AA.
AC   P29147; Q5U2Q2;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 2.
DT   25-NOV-2008, entry version 70.
DE   RecName: Full=D-beta-hydroxybutyrate dehydrogenase, mitochondrial;
DE            Short=BDH;
DE            EC=1.1.1.30;
DE   AltName: Full=3-hydroxybutyrate dehydrogenase;
DE   Flags: Precursor;
GN   Name=Bdh1; Synonyms=Bdh;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=Sprague-Dawley; TISSUE=Liver;
RX   MEDLINE=92232670; PubMed=1567834; DOI=10.1021/bi00130a009;
RA   Churchill P., Hempel J., Romovacek H., Zhang W.W., Churchill S.,
RA   Brennan M.;
RT   "Primary structure of rat liver D-beta-hydroxybutyrate dehydrogenase
RT   from cDNA and protein analyses: a short-chain alcohol dehydrogenase.";
RL   Biochemistry 31:3793-3799(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY: (R)-3-hydroxybutanoate + NAD(+) = acetoacetate
CC       + NADH.
CC   -!- ENZYME REGULATION: Requires phosphatidylcholine as an allosteric
CC       activator for enzymatic activity (By similarity).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; M89902; AAB59684.1; ALT_INIT; mRNA.
DR   EMBL; BC085916; AAH85916.1; ALT_INIT; mRNA.
DR   PIR; A42345; A42345.
DR   RefSeq; NP_446447.2; -.
DR   UniGene; Rn.36635; -.
DR   HSSP; P14061; 1FDS.
DR   Ensembl; ENSRNOG00000001736; Rattus norvegicus.
DR   GeneID; 117099; -.
DR   KEGG; rno:117099; -.
DR   NMPDR; fig|10116.3.peg.7406; -.
DR   RGD; 620131; Bdh1.
DR   HOVERGEN; P29147; -.
DR   NextBio; 619980; -.
DR   ArrayExpress; P29147; -.
DR   GermOnline; ENSRNOG00000001736; Rattus norvegicus.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0003858; F:3-hydroxybutyrate dehydrogenase activity; IEA:EC.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DHase_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Allosteric enzyme; Direct protein sequencing;
KW   Mitochondrion; NAD; Oxidoreductase; Transit peptide.
FT   TRANSIT       1     46       Mitochondrion.
FT   CHAIN        47    343       D-beta-hydroxybutyrate dehydrogenase,
FT                                mitochondrial.
FT                                /FTId=PRO_0000031962.
FT   NP_BIND      59     83       NAD (By similarity).
FT   ACT_SITE    208    208       Proton acceptor (By similarity).
FT   BINDING     195    195       Substrate (By similarity).
FT   MOD_RES     132    132       N6-acetyllysine (By similarity).
FT   MOD_RES     275    275       N6-acetyllysine (By similarity).
FT   CONFLICT     90     91       DK -> EQ (in Ref. 1; AAB59684).
SQ   SEQUENCE   343 AA;  38202 MW;  6903D2ED27F2B055 CRC64;
     MLAARLSRPL SQLPGKALSV CDRENGTRHT LLFYPASFSP DTRRTYTSQA DAASGKAVLV
     TGCDSGFGFS LAKHLHSKGF LVFAGCLLKD KGDAGVRELD SLKSDRLRTI QLNVCNSEEV
     EKAVETVRSG LKDPEKGMWG LVNNAGISTF GEVEFTSMET YKEVAEVNLW GTVRTTKSFL
     PLLRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMHPLG VKVSVVEPGN
     FIAATSLYSP ERIQAIAKKM WDELPEVVRK DYGKKYFDEK IAKMETYCNS GSTDTSSVIN
     AVTHALTAAT PYTRYHPMDY YWWLRMQVMT HFPGAISDKI YIH
//